Baran Can Sağlam

Evaluation of Microcrack Formation in Root Canals After Instrumentation With different NiTi Rotary File Systems: A Scanning Electron Microscopy Study

The aim of this study was to evaluate the dentinal microcrack formation of ProTaper Universal, ProTaper Next, and WaveOne. Sixty extracted mandibular molars were selected. The mesial roots were resected and randomly divided into four groups (n = 15). The canals were prepared with hand files (group 1), ProTaper Universal (group 2), ProTaper Next (group 3), and WaveOne (group 4) instrument systems. The roots were separated horizontally at 3, 6, and 9 mm from the apex. Digital images were captured at ×40 magnification using scanning electron microscopy to detect microcrack formation. Statistical analysis was performed by Pearson Chi-square test. The prevalence of microcracks in group 2, group 3, and group 4 were significantly higher when compared to group 1 (p < 0.001). Group 2, group 3, and group 4 demonstrated similar prevalence of microcracks without significant difference (p > 0.05) in all sections. All instruments caused microcracks except for hand file. The highest percentage of microcrack was recorded in 3 mm section for all groups.

Effect of light-activated bleaching on pulp chamber temperature rise: An in vitro study

This study aimed to compare the pulp chamber temperature changes that occur with the use of different light sources during vital bleaching with and without application of bleaching agents. One hundred and forty-four mandibular incisor teeth were divided into four groups (n = 36) according to the use of halogen light, light-emitting diode, 3 W and 1.5 W diode laser. The teeth in the main groups were divided into three subgroups (n = 12). First subgroup had no bleaching gel application. By White and Whiteness HP were applied with a thickness of 2 mm to the other subgroups respectively. The labial surfaces of the teeth were irradiated with a total time of 20 s. Temperature changes in the pulp chamber were measured and analysed by using anova and Tukey test. The 3 W diode laser induced the highest pulp chamber temperature rise (P = 0.000). Bleaching gel application reduced the temperature changes in the diode laser groups (P < 0.05). Diode laser activation during vital bleaching induces pulp chamber temperature rise, which may cause thermal tissue damage.

Effects of sodium hypochlorite on gutta-percha and Resilon cones: An atomic force microscopy and scanning electron microscopy study

The aim of this study was to evaluate the effects of 6% sodium hypochlorite (NaOCl) on gutta-percha (GP) and Resilon cones. Six standardized GP and Resilon cones were selected and cut 3mm from their tip. One GP and 1 Resilon cone were used as control samples. Cones were immersed in 6% NaOCl for 1, 5, 10, 20, and 30 minutes, thoroughly rinsed with nanopure water, and dried with filter paper. Then, surface topography was analyzed by atomic force microscopy and scanning electron microscopy coupled to an energy-dispersive x-ray (EDX) spectrometer. According to the root mean square and depth analysis values obtained from atomic force microscopic evalution, there were no significant differences found among the GP groups. However significant differences were found among Resilon cones (P ≤ .05). SEM images and EDX graphics showed that there were no prominent differences between GP and Resilon groups. These results showed that 6% NaOCl solution can be used in the disinfection of GP and Resilon cones. No alterations were observed on the GP cones, but it can change the surface of Resilon cones.

Efficacy of different solvents in removing gutta-percha from curved root canals: a micro-computed tomography study.

The aim of this study was to compare the root filling material that remained after retreatment of curved root canals with chloroform and Endosolv R as solvents. The evaluation employed micro-computed tomography (CT) imaging. Thirty-six extracted molar teeth with curved roots were selected. After preparation with ProTaper rotary instruments, the canals were filled with gutta-percha and AH26 sealer. The teeth were randomly divided into three groups according to solvent used (n = 12) as follows: group 1: chloroform; group 2: Endosolv R; group 3: no solvent (negative control). ProTaper Universal Retreatment files were used to remove each root canal filling and then the self-adjusting file was applied for two minutes. Preoperative and postoperative micro-CT images were used to assess the percentage of residual filling material. The mean percentage of residual filling material was quantified. There were no statistically significant differences between the groups in terms of percentage volume of residual root canal filling.

Efficiency of 2 electronic apex locators on working length determination: A clinical study.

Aims: The aim of this clinical study was to evaluate the clinical accuracy of two electronic apex locators (EALs). Materials and Methods: A total of 120 patients with 283 roots were randomized into three groups including, traditional radiographic method, EAL (Root ZX mini), and apex locating endodontic motor (VDW Gold) for working length (WL) determination. Root canals were instrumented to a size ProTaper F3 nickel titanium file. The obturation quality of matched tapered master cone (ProTaper F3) was determined for the accuracy of WL. Statistical Analysis Used: Descriptive statistics were expressed as numbers and percentages. Pearson Chi-square test was used to determine for differences between groups. P < 0.05 was considered statistically significant for all tests. Results: There was no statistically significant difference between the three tested groups (P = 0.894). Conclusions: The success of both apex locators was similar to the radiographic WL determination technique.

Effect of post‐space treatments on the push‐out bond strength and failure modes of glass fibre posts

The aim of this study was to evaluate the effect of different post-space treatments on the push-out bond strength and failure modes of glass fibre posts. Forty mandibular premolar roots were cut and endodontically treated. Post spaces were prepared and roots were divided into four groups. In group 1 distilled water irrigation (control), in group 2 2.25% NaOCl irrigation, in group 3 2.25% NaOCl + 17% EDTA irrigation were done and in group 4 diode laser was applied to the prepared post spaces. Scanning electron microscope (SEM) analysis was made for each group. Fibre posts were then luted with resin cement. Each root was prepared for push-out test. Data were statistically analysed with anova (P = 0.05). After push-out test, the failure modes were observed but not statistically analysed. There were statistically significant differences between Group 3 and Group 2 in both regions (P < 0.05), also in the middle region of Group 4 and Group 2 (P < 0.05). Cervical root segments showed higher bond strengths than middle ones in all groups (P < 0.05). The highest bond strength values were obtained from NaOCl/EDTA and the lowest bond strength values were obtained from NaOCl for both regions.

The effect of glide path on the surface quality of new and used rotary and reciprocating single files: OneShape versus WaveOne

The aim of this study was to evaluate the effect of glide path preparation on the surface topography changes of WaveOne and OneShape single files using atomic force microscopy analysis. Two commercially available brands of single file systems were analyzed, including 15 specimens/brand as following: Group I; WaveOne 0.08/25 (Dentsply Maillefer, Ballaigues, Switzerland) and Group II; OneShape 0.06/25 (Micro Mega, Besançon, France). In each group, 3 new files were subjected to AFM analysis as received from manufacturer, and 12 files were analyzed after preparation of root canals. A total of 20 human maxillary molar teeth were selected. In each group, 12 files were divided into 4 subgroups according to instruments that were used in one or three root canals with or without a previous glide path (n = 3). After preparation the surface analysis of single files was performed on 11 different points located along a 3-mm section starting at the tip of each file using AFM. Root mean square (RMS) values were recorded to investigate the surface features of endodontic files. The differences between RMS values were tested by one-way ANOVA. There was no statistically significant difference between the groups (p > 0.05). Creation of a glide path did not affect the surface topography of single file systems.

Comparison of calcium hydroxide removal by self-adjusting file, EndoVac, and CanalBrush agitation techniques: An in vitro study.

Objectives: This study comparatively evaluated the efficacy of self-adjusting file (SAF), Endovac, and CanalBrush irrigant agitation protocols in removing calcium hydroxide (Ca(OH)2) from the root canals. Materials and Methods: Sixty extracted human mandibular canine teeth were instrumented with ProTaper rotary instruments to size #40 and dressed with Ca(OH)2. The roots were randomly assigned to four groups according to irrigant agitation protocol used (n = 15). In Group 1: Conventional syringe irrigation (no activation, control); Group 2: Rotary brush agitation (CanalBrush); Group 3: Apical negative pressure irrigation (EndoVac system); and Group 4: Sonic agitation (SAF) were used. Scanning electron microscopic (SEM) evaluation was done for assessment of Ca(OH)2 removal in the coronal and apical thirds. Statistical analysis was performed by Wilcoxon and Kruskal-Wallis tests. Results: There were statistically significant differences among the groups (P = 0.218). A statistically significant difference was seen between the test groups in Ca(OH)2 removal from the apical third of the canal (P < 0.05). In the coronal third, there was no difference between the groups (P > 0.05). The most efficient Ca(OH)2 removal in apical third was recorded in Group 3 (EndoVac) and Group 4 (SAF) (P < 0.05). In Group 4 (sonic agitation), there was no significantly difference between Ca(OH)2 removal in coronal and apical thirds. Conclusions: SAF and EndoVac showed significantly better performance than CanalBrush and conventional syringe irrigation in removing Ca(OH)2 from apical third of the root canals.

Effects of irrigation solutions on the surface of protaper instruments: A microscopy study

The aim of this study was to evaluate the effects of sodium hypochlorite (NaOCl), chlorhexidine digluconate (CHX), and mixture of tetracycline, citric acid, and detergent (MTAD) solutions on the surfaces of nickel titanium (NiTi) rotary files with scanning electron microscopy (SEM) and atomic force microscopy (AFM). Irrigation solutions including 2.5% NaOCl, 5% NaOCl, 2% CHX, and MTAD were used. Four new ProTaper rotary NiTi files (F3) were immersed in each four irrigation solutions for 10 min, separately. One untreated NiTi file was used as a control specimen. The surface analysis was performed with SEM and AFM. The surface analysis in AFM was performed on 12 different regions located between first and second millimeters from the tip. SEM images were taken with different magnifications. No surface alteration was recorded under SEM evaluation. AFM revealed that the root mean square (RMS) values of all treated specimens were statistically higher than the control sample (P < 0.05). The sample which was treated with CHX showed lowest RMS values in test groups. All tested solutions caused surface alterations. CHX demonstrated limited surface alterations when compared to the other tested solutions. Microsc. Res. Tech.

Cleaning efficacy of reciprocal and rotary systems in the removal of root canal filling material

Aim: The aim of this study was to compare the efficacy of hand file, nickel titanium rotary instrument, and two reciprocating instruments for removing gutta-percha and sealer from the root canals. Materials and Methods: Eighty-eight mandibular premolar teeth were used. The root canals were shaped and filled with gutta-percha and a resin-based sealer. The specimens were divided into four groups according to the technique by which the root filling material was removed: Group 1 - Wave One; Group 2 - Reciproc; Group 3 - ProTaper; and Group 4 - Gates-Glidden burs and stainless steel hand file. Then teeth were split longitudinally and photographed. The images were transferred to a computer. The ratio of remaining filling material to the root canal periphery was calculated with the aid of ImageJ software. Statistical analysis was performed using Kruskal-Wallis and Mann-Whitney tests. Results: A significant difference was found among all groups (P < 0.001). The WaveOne group demonstrated significantly less remaining filling material. The greatest amount of filling material was found in specimens where gutta-percha was removed with Gates-Glidden burs and stainless steel hand file. Conclusion: The reciprocating files were found to be significantly more effective in removing the filling material from the canal walls compared to the rotational file and hand file.