Basuki B. Purnomo

Inhibitory Effect of Silodosin, Tamsulosinand Terazosin in Phenylephrine Induced-Prostate Smooth MuscleContraction (a Comparative in vitro Study)

Objective: This in vitro study aims to compare the inhibitory effect of those α-blocker to the phenylephrine inducedprostate smooth muscle contraction. Research Method: Twenty eight eligible BPH patients were randomly allocated to 4 groups which are one control group (without α-blocker therapy) and 3 intervention groups treated with silodosin, tamsulosinand terazosine for 7 days prior to TURP surgery. The prostate tissue samples were taken during TURP surgery. This sample prepared on organ bath containing thyrod liquid and further induced with phenilephrin at dose 104μg/ml to measure its contraction. A oneway anova analysis was performed to investigate the significant difference among groups. Result: The mean contraction result in control, silodosin, tamsulosinand terazosin groups were 7,798mV, 1,718mV, 3,416mV, and 5,956 mV respectively (Table 1). There was a significant difference contraction value among 4 groups(p=0,000). The silodosin treatment group had the lowest mean contraction value against the controls compared with the tamsulosin and terazosin groups. Conclusion: Silodosin has the strongest inhibitory effect in phenylephrineinduced prostate smooth muscle contraction in the invitro study.

THE N-HEXANE FRACTION OF KESUM (Polygonum Minus L.) INDUCE APOPTOSIS THE LUNG EPITHELIAL CELLS OF THE RATUS NOVERGICUS THAT EXPOSED BY BENZOPYRENE

Kesum (Polygonum minus L.) is a popular plant and is often used as additives in many typical dishes in the West Kalimantan. These plants belong to the family Polygonaceae, and potential to use in the prevention and treatment of lung cancer. This research aimed at studying the effect of giving the n-hexane fraction of the kesum to induces of apoptosis in epithelial cells of lung tissue of the animal models that exposed by benzopyrene. research was conducted on three groups of animal models: i) the healthy group, ii) the group treated by benzopyrene 200 mg/kg, and iii) the therapy group by n-hexane fraction 100 mg/kg. The results of the study then carried measurements of lung epithelial cell apoptosis index and measurements of lung ROS levels. The results showed that n-hexane fraction able to reduce of lung ROS levels of the animal models that exposed by benzopyrene with MDA levels respectively are 5.09 ± 0.76 ppm for the healthy group, 8.44 ± 1.89 ppm for the group treated by benzopyrene, and 5.47 ± 1.76 ppm for the therapy group. The n-hexane fraction also able to induce apoptosis of the lung epithelial cells of the animal models that exposed by benzopyrene with apoptosis index values respectively are 2.61 ± 1.98 for the healthy group, 7.41 ± 2.26 for the group treated by benzopyrene, and 16.31 ± 5.73 for the therapy group.