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Featured researches published by Bettina Huber.


Bioresource Technology | 2017

High performance biological methanation in a thermophilic anaerobic trickle bed reactor

D. Strübing; Bettina Huber; M. Lebuhn; Jörg E. Drewes; K. Koch

In order to enhance energy efficiency of biological methanation of CO2 and H2, this study investigated the performance of a thermophilic (55°C) anaerobic trickle bed reactor (ATBR) (58.1L) at ambient pressure. With a methane production rate of up to 15.4m3CH4/(m3trickle bed·d) at methane concentrations above 98%, the ATBR can easily compete with the performance of other mixed culture methanation reactors. Control of pH and nutrient supply turned out to be crucial for stable operation and was affected significantly by dilution due to metabolic water production, especially during demand-orientated operation. Considering practical applications, inoculation with digested sludge, containing a diverse biocenosis, showed high adaptive capacity due to intrinsic biological diversity. However, no macroscopic biofilm formation was observed at thermophilic conditions even after 313days of operation. The applied approach illustrates the high potential of thermophilic ATBRs as a very efficient energy conversion and storage technology.


Water Science and Technology | 2014

Revealing biogenic sulfuric acid corrosion in sludge digesters: detection of sulfur-oxidizing bacteria within full-scale digesters

Bettina Huber; Jörg E. Drewes; K. C. Lin; R. König; Elisabeth Müller

Biogenic sulfuric acid corrosion (BSA) is a costly problem affecting both sewerage infrastructure and sludge handling facilities such as digesters. The aim of this study was to verify BSA in full-scale digesters by identifying the microorganisms involved in the concrete corrosion process, that is, sulfate-reducing (SRB) and sulfur-oxidizing bacteria (SOB). To investigate the SRB and SOB communities, digester sludge and biofilm samples were collected. SRB diversity within digester sludge was studied by applying polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) targeting the dsrB-gene (dissimilatory sulfite reductase beta subunit). To reveal SOB diversity, cultivation dependent and independent techniques were applied. The SRB diversity studies revealed different uncultured SRB, confirming SRB activity and H2S production. Comparable DGGE profiles were obtained from the different sludges, demonstrating the presence of similar SRB species. By cultivation, three pure SOB strains from the digester headspace were obtained including Acidithiobacillus thiooxidans, Thiomonas intermedia and Thiomonas perometabolis. These organisms were also detected with PCR-DGGE in addition to two new SOB: Thiobacillus thioparus and Paracoccus solventivorans. The SRB and SOB responsible for BSA were identified within five different digesters, demonstrating that BSA is a problem occurring not only in sewer systems but also in sludge digesters. In addition, the presence of different SOB species was successfully associated with the progression of microbial corrosion.


Waste Management | 2016

Methane from CO2: Influence of different CO2 concentrations in the flush gas on the methane production in BMP tests

K. Koch; Bettina Huber; Yadira Bajón Fernández; Jörg E. Drewes

The influence of carbon dioxide (CO2) in the headspace gas on the specific methane (CH4) production of blank samples with just inoculum during Biochemical Methane Potential (BMP) tests was studied. The headspace of the bottles had been flushed with 15 different ratios of CO2 and N2 prior to incubation, while they were treated otherwise identically. The results revealed that the CH4 yield increased linearly with higher ratio of CO2 in the flush gas reaching a 30% higher yield at pure CO2 relative to pure N2 headspace conditions. However, a slightly distinct lag is noticeable during the initial phase of the degradation process at high ratios of CO2, hypothesizing a reversible disturbance of the biocenosis. Further experiments and analyses need to be performed to elucidate the underlying mechanisms.


Environmental Science and Pollution Research | 2014

Xenobiotic benzotriazoles—biodegradation under meso- and oligotrophic conditions as well as denitrifying, sulfate-reducing, and anaerobic conditions

Bastian Herzog; Hilde Lemmer; Bettina Huber; Harald Horn; Elisabeth Müller


BMC Microbiology | 2016

Characterization of sulfur oxidizing bacteria related to biogenic sulfuric acid corrosion in sludge digesters

Bettina Huber; Bastian Herzog; Jörg E. Drewes; K. Koch; Elisabeth Müller


Cement and Concrete Research | 2016

Comparative analysis of biogenic and chemical sulfuric acid attack on hardened cement paste using laser ablation-ICP-MS

Bettina Huber; H. Hilbig; Mariana M. Mago; Jörg E. Drewes; Elisabeth Müller


Environmental Sciences Europe | 2013

Analysis and in situ characterization of activated sludge communities capable of benzotriazole biodegradation

Bastian Herzog; Bettina Huber; Hilde Lemmer; Harald Horn; Elisabeth Müller


Cement and Concrete Research | 2017

Evaluation of concrete corrosion after short- and long-term exposure to chemically and microbially generated sulfuric acid

Bettina Huber; H. Hilbig; Jörg E. Drewes; E. Müller


International Conference “Progress in Biogas IV” | 2017

Microbial methanation of H2/CO2 in anaerobic trickle bed reactors – A study of microbial biocenosis and nutrient supply strategies under thermophilic conditions

D. Strübing; Bettina Huber; M. Lebuhn; Jörg E. Drewes; K. Koch


Acta Zoologica | 2017

Microvascularization of the thyroid glands in larval and adult Xenopus laevis‐ histomorphology and scanning electron microscopy of vascular corrosion casts

Mathias Hopfinger; Bettina Huber; Alois Lametschwandtner

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Harald Horn

Karlsruhe Institute of Technology

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