Byung-Gil Jung

Isolation and characterization of Pseudomonas otitidis WL-13 and its capacity to decolorize triphenylmethane dyes

Pseudomonas otitidis WL-13, which has a high capacity to decolorize triphenylmethane dyes, was isolated from activated sludge obtained from a wastewater treatment plant of a dyeing industry. This strain exhibited a remarkable color-removal capability when tested against several triphenylmethane dyes under both shaking and static conditions at high concentrations of dyes. More than 95% of Malachite Green and Brilliant Green was removed within 12 h at 500 micromol/L dye concentration under shaking conditions. Crystal Violet lost about 13% of its color under the same conditions tested. The rate of decolorization increased when the M9 medium was supplemented with yeast extract. The optimum pH and temperature for color removal were 7-9 and 35-40 degrees C, respectively. The observed changes in the visible spectra and the inspection of bacterial growth indicated the color-removal by the adsorption of dye to the cells during incubation with strains.

Characterization of Petroleum Hydrocarbon Degradation by a Sphingomonas sp. 3Y Isolated from a Diesel-Contaminated Site.

Bacterial stain 3Y was isolated from a site that was contaminated with diesel for more than 15 years. The strain could grow on various petroleum using hydrocarbons as the sole carbon source. The strain grew not only on aliphatic hydrocarbons but also on aromatic hydrocarbons. 3Y grew on aliphatic petroleum hydrocarbons hexane or hexadecane, and aromatic petroleum hydrocarbons BTEX, phenol, biphenyl, or phenanthrene. The strain showed aromatic ring dioxygenase and meta-cleavage dioxygenase activities as determined by tests using indole and catechol. Aromatic ring dioxygenase is involved in the initial step of biodegradation of aromatic hydrocarbons while meta-cleavage dioxygenase catalyzes the cleavage of the benzene ring. Based on a nucleotide sequence analysis of its 16S rRNA gene, 3Y belongs to the genus Sphingomonas. A phylogenetic tress was constructed based on the nucleotide sequences of closest relatives of 3Y and petroleum hydrocarbon degrading sphingomonads. 3Y was in a cluster that was different from the cluster that contained well-known sphingomonads. The results of this study suggest that 3Y has the potential to cleanup oil-contaminated sites. Further investigation is warranted to optimize conditions to degrade petroleum hydrocarbons by the strain to develop a better bioremediation strategy.

A Study on the Treatment of Oil Contaminated Soils with Micro-nano Bubbles Soil Washing System

The objectives of this study are to examine the processing of oils contamination soil by means of using a micro- nano-bubble soil washing system, to investigate the various factors such as washing periods, the amount of mi- cro-nano bubbles generated depending on the quantity of acid injection and quantity of air injection, to examine the features involved in the elimination of total petroleum hydrocarbons (TPHs) contained in the soil, and thus to eval- uate the possibility of practical application on the field for the economic feasibility. The oils contaminated soil used in this study was collected from the 0~15 cm surface layer of an automobile junkyard lo- cated in U City. The collected soil was air-dried for 24 hours, and then the large particles and other substances contained in the soil were eliminated and filtered through sieve No.10 (2 mm) to secure consistency in the samples. The TPH concentration of the con- taminated soil was found to be 4,914~5,998 mg/kg. The micronano-bubble soil washing system consists of the reactor, the flow equalization tank, the micronano- bub- ble generator, the pump and the strainer, and was manufactured with stainless material for withstanding acidic phase. When the injected air flow rate was fixed at 2 L/min, for each hydrogen peroxide concentrations (5, 10, 15%) the removal percents for TPH within the contaminated soil with retention times of 30 minutes were respectively identi- fied as 4,931 mg/kg (18.9%), 4,678 mg/kg (18.9%) and, 4,513 mg/kg (17.7%). And when the injected air flow rate was fixed at 2 L/min, for each hydrogen peroxide concentrations (5, 10, 15%) the removal percents for TPH within the contaminated soil with retention times of 120 minutes were respectively identified as4,256 mg/kg (22.3%), 4,621 mg/kg (19.7%) and 4,268 mg/kg (25.9%).

Estimation of Kinetic Parameters for Biomass Growth Using Micro-nano Bubbles Reactor

The objectives of this research are to evaluate and compare the oxygen transfer coefficients(KLa) in both a general bubbles reactor and a micro-nano bubbles reactor for effective operation in sewage treatment plants, and to understand the effect on microbial kinetic parameters of biomass growth for optimal biological treatment in sewage treatment plants when the micro-nano bubbles reactor is applied. Oxygen transfer coefficients(KLa) of tap water and effluent of primary clarifier were determined. The oxygen transfer coefficients of the tap water for the general bubbles reactor and micro-nano bubbles reactor were found to be 0.28 hr -1 and 2.50 hr -1 , respectively. The oxygen transfer coefficients of the effluent of the primary clarifier for the general bubbles reactor and micro-nano bubbles reactor were found be to 0.15 hr -1 and 0.91 hr -1 , respectively. In order to figure out kinetic parameters of biomass growth for the general bubbles reactor and micro-nano bubbles reactor, oxygen uptake rates(OURs) in the saturated effluent of the primary clarifier were measured with the general bubbles reactor and micro-nano bubbles reactor. The OURs of in the saturated effluent of the primary clarifier with the general bubbles reactor and micro-nano bubbles reactor were 0.0294 mg O2/L․hr and 0.0465 mg O2/L․hr, respectively. The higher micro-nano bubbles reactors oxygen transfer coefficient increases the OURs. In addition, the maximum readily biodegradable substrate utilization rates(Kms) for the general bubbles reactor and micro-nano bubbles reactor were 3.41 mg COD utilized/mg active VSS․day and 7.07 mg COD utilized/mg active VSS․day, respectively. The maximum specific biomass growth rates for heterotrophic biomass(μmax) were calculated by both values of yield for heterotrophic biomass(YH) and the maximum readily biodegradable substrate utilization rates(Kms). The values of μmax for the general bubbles reactor and micro-nano bubbles reactor were 1.62 day -1 and 3.36 day -1 , respectively. The reported results show that the micro-nano bubbles reactor increased air-liquid contact area. This method could remove dissolved organic matters and nutrients efficiently and effectively.

Removal Characteristics of TPHs and Heavy Metals in Contaminated Soil with Ultrasonic Washing

The removal characteristics of total petroleum hydrocarbons (TPHs) and heavy metals in contaminated soils with ultrasonic washing have been studied. The ultrasonic washing was evaluated on a laboratory scale. In this investigation, the effects of factors such as ultrasonic frequency, power intensity, duration of irradiation, contents of the TPHs and heavy metals and mixing ratios between the contaminated soils and water, were considered. Experimental results suggested that the rates for contaminant extraction of the TPHs and heavy metals in the contaminated soil increased considerably with the ultrasonic washing. Therefore, the ultrasonic washing has previously been to be an effective method to remediate the contaminated soils with the TPHs and heavy metals.

Reclamation of Effluent Textile Wastewater Using Micro/nano Bubbles-Dissolved Ozone Flotation Process

The main objectives of this research are to investigate characteristics of ozone solubility due to low solubility of conven- tional bubbles-ozone generators, evaluate the treatment characteristics of reclaiming textile wastewater for industrial water by means of micro/nano bubbles-dissolved ozone flotation(MNB-DOF) process. The textile wastewater used in this research was obtained from final effluent of the textile wastewater in B city. There is a 400L reactor which consists of a micro-nano bubble system and a ozone generator for experiments. As a result of generating micro-nano bubbles (below 0.5 ㎛) by using of MNB-DOF process, it improved ozone solubility due to higher ozone transfer rates. Consequently, the shorter ozonation time clearly indicates the lower power costs. The reported results clearly indicated that MNB-DOF process can be effectively and inexpensively. Results of the experiments through MNB-DOF process in this study satisfy all reclaiming standards as in- dustrial water: pH 6.5~8.5, SS 10 mg/L or below, BOD5 6 mg/L or below, turbidity 10 NTU or below, Coliforms 1,000/100 mL or below. Therefore there is a possibility of the reclaiming of the textile wastewater as industrial water.

Manufacturing Characteristics of Environmental-friendly Waste Ash Brick with Industrial By-Products

The main objective of this study was to evaluate the effects on shape and size, compressive strength, water absorption and heavy metals leaching with various weight mixing ratios in waste ash brick products using waste recycling MSWI(Municipal Solid Waste Incinerator) bottom ash, steel slag and waste building material. The manufacturing processes for the waste ash brick consist of screening, mixing, conveyor transmission, compaction.forming, and curing steps of raw materials. The weight mixing ratios of steel slag around bottom ash were adjusted within the ranges of 10% to 30%. The reported results show that the width and thickness of the manufactured waste ash brick could be satisfied with , respectively which are K.S. standards of products qualities. And in case of length, only 20-Ba50Ss30, 20-Ba60Wb20 and 20-Ba50Wb30 for the mixing ratios could be satisfied with that is K.S. standards of products quality. The compressive strength and water absorption for 20-Ba50Ss30 and 20-Ba70Wb10 were over and below 15% respectively that are K.S. standards of manufactured waste ash brick. The results of tests for the heavy metals leaching in the all manufactured waste ash bricks are also passed to the wastes management regulations. The cost analysis of 20-Ba50Ss30 is evaluated. The manufacturing cost is evaluated 34.3 won/brick with 8 hours and 20tons of raw material per day. Incinerators with problems in bottom ash disposal can therefore derive significant benefits from the application of waste ash brick production.

The Investigation Study of Compaction Density by Waste Composition Change in Landfill Site

The purpose of this study is to investigate weighted compaction density according to a leading density in truck, a compaction density of solid waste and composition ratios of solid waste fur calculation of a capacity of the landfill sites. The experiments for calculations of in-place density at landfill site have been conducted in S landfill site at B City. The size of vessel for measuring the compaction density was . The experiment tests have been carried out methods (1 time for bulldozer and 4 times for compactor) that do contain all of specification at the landfill site. Average of the loading density at the landfill site was (). When the loading density for each compositions was compared, the composition of the highest average loading density () was miscellaneous wastes. The composition of the lowest average loading density () was general solid waste. The reported results indicated that the compaction density at the landfill site was , which was calculated with weighted incoming ratios of compositions. The ranges of the density for each composition were from to . When the compaction density for each composition was compared, the composition with the highest average compaction density () was miscellaneous wastes. The composition with the lowest average compaction density () was general solid wastes.

Enhanced TPH Degradation of Diesel-Contaminated Soil by Microwave Heating

The application of microwave technology has been investigated in the remediation of diesel-contaminated soil. The paper deals with economic assessment by means of cost analysis and degradation characteristics at different microwave powers for total petroleum hydrocarbon (TPH) in diesel contaminated soils. The soils from S Mountain around the D University were sampled. The samples were screened with 2.0 mm mesh and dried for 6 hours before the diesel was added into the dried soils. The diesel-contaminated soil (3,300 mg THP/kg soil) was prepared with diesel (S Co.). The drying process was carried out in a microwave oven, a standard household appliance with a 2,450 MHz frequency and 700 W of power. The experiments were conducted from 0 to 20 minutes as the microwave powers increased from 350W to 500W to 700W. The concentrations of TPH were analysed using a gas chromatography/mass spectrometer (GC/MS). The initial concentration of TPH was 3,300 mg TPH/kg soil. The weight of contaminated soil was 200g. The concentration of TPH was decreased to 1,828 mg TPH/kg soil (44.7%), 1,347 mg TPH/kg soil (59.2%) and 1,014 mg TPH/kg soil (69.3%) at 350W, 500W and 700W for 15 minutes respectively. In addition, the curve was best fit with first order kinetics using the least-square method. The ranges of a first order rate constant k and r-square were and respectively.

Pretreatment Characteristics and Specific Methanogenic Activity of Municipal Sewage Sludge by Dual Frequency Sonication

The objectives of this study have been carried out to investigate the solubilization of municipal sewage sludge by single and dual frequency ultrasonic pretreatment, and the methane production characteristics of pretreated sewage sludge by specific methanogenic activity test for sewage sludge reduction. The waste activated sludge was collected from thickened tank of Suyoung sewage treatment plant in Busan city, and its concentration was adjusted to 1.0% total solids. Ultrasonic frequency was varied 15, 20, 15+20 kHz, and acoustic density was used a maximum 176W/L. The dual frequency ultrasonic pretreatment was found to be more effective than single frequency ultrasonic in the solubilization rate and methane production. The , rate were 15.2%, 13.9%, 17.0% with single frequency of 15 kH2, 20 kHz, dual frequency of 15+20 kHz, respectively. The application of dual frequency ultrasound for sewage sludge pretreatment can be interest for sewage treatment plants having problems in sludge treatment and disposal.