C.N. Novikov

Chemiluminescence during respiratory burst in nondiluted human blood can be enhanced by back-reflected photons

We have demonstrated recently that luminol- or lucigenin- dependent chemiluminescence (LM-CL and LC-CL, respectively) registered from whole non-diluted blood may reach rather high intensities. Here we demonstrate, that parameters of both LM-CL and LC-CL in non-diluted human blood during RB development is influenced by a reflective screen of aluminum foil surrounding a test tube with a blood sample. Photons reflected back to a sample accelerate slowly developing and retard rapidly development RB. As demonstrated by histochemical NBT test, wrapping samples with blood in aluminum foil effected also the reductive neutrophil activity in. These results indicate that weak light fluxes generated in the course of RB may influence physiological processes in blood. They also provide the basis for an explanation for the phenomenon of the interaction two blood samples in one of which RB in the presence of luminol was stimulated through the optical channel previously reported by us.