Carlos Eduardo Gamero Aguilar

Comparative genomic survey of Bacillus cereus sensu stricto isolates from the dairy production chain in Brazil

Abstract The genomes of 262 Bacillus cereus isolates were analyzed including 69 isolates sampled from equipment, raw milk and dairy products from Brazil. The population structure of isolates showed strains belonging to known phylogenetic groups II, III, IV, V and VI. Almost all the isolates obtained from dairy products belonged to group III. Investigation of specific alleles revealed high numbers of isolates carrying toxin‐associated genes including cytK (53.62%), hblA (59.42%), hblC (44.93%), hblD (53.62%), nheA (84.06%), nheB (89.86%) and nheC (84.06%) with isolates belonging to groups IV and V having significant higher prevalence of hblACD and group IV of CytK genes. Strains from dairy products had significantly lower prevalence of CytK and hblACD genes compared to isolates from equipment and raw milk/bulk tanks. Genes related to sucrose metabolism were detected at higher frequency in isolates obtained from raw milk compared to strains from equipment and utensils. The population genomic analysis demonstrated the diversity of strains and variability of putative function among B. cereus group isolates in Brazilian dairy production, with large numbers of strains potentially able to cause foodborne illness. This detailed information will contribute to targeted interventions to reduce milk contamination and spoilage associated with B. cereus in Brazil. Figure. No Caption available.

Spatial distribution, prevalence and epidemiological risk factors of cysticercosis in cattle from state of São Paulo, Brazil, slaughtered for human consumption

This present research focused on establishing the prevalence, geospatial distribution and epidemiological risk factors for bovine cysticercosis in the state of São Paulo, Brazil, in cattle slaughtered for human comsumption. Data about the inspection of 104,180 bovine carcasses from 215 farms and from 70 municipalities were gathered between January and December of 2012. A cluster analysis was performed in order to correlate some variables: prevalence of bovine cysticercosis, total coffee harvesting area (hectares), orange producing areas (hectares) and Human Development Index. Afterwards, distribution maps were created in order to help the results interpretation. An average prevalence of 2.9% was established in the state of São Paulo during the studied period. The Administrative regions of São José do Rio Preto and Campinas had higher risk for cysticercosis (OR >1 and 95% CI >1). The cluster analysis showed a grouping (G1 cluster) of the variables: prevalence of bovine cysticercosis, total area of sugar cane harvested, total area of orange harvested and total area of coffee harvested. This agrroupment allows us to infer that cyticercosis cases in this region are correlated with those variables distribution. Such aspects indicate that the presence of temporary rural workers and other socioeconomic and cultural features in each region can contribute to bovine cysticercosis dissemination in some areas.

Implementation and evaluation of biosecurity programs in pig production. A Review

Univ Estadual Paulista, UNESP, Fac Ciencias Agr & Vet Jaboticabal, Programa Posgrad Med Vet, Sao Paulo, Brazil