Celice Alexandre Silva

Severe hemorrhagic corpus luteum complicating anticoagulation in antiphospholipid syndrome.

Antiphospholipid syndrome (APS) is a disorder of coagulation that causes thrombosis as well as pregnancy-related complications, occurring due to the autoimmune production of antibodies against phospholipid. Full anticoagulation is the cornerstone therapy in patients with thrombosis history, and this can lead to major bleeding. During a 3-year period, 300 primary and secondary APS patients were followed up at the Rheumatology Division of the authors’ University Hospital. Of them, 255 (85%) were women and 180 (60%) were of reproductive age. Three of them (1%) had severe hemorrhagic corpus luteum while receiving long-term anticoagulation treatment and are described in this report. All of them were taking warfarin, had elevated international normalized ratio (>4.0) and required prompt blood transfusion and emergency surgery. Therefore, we strongly recommend that all women with APS under anticoagulation should have ovulation suppressed with either intramuscular depot-medroxyprogesterone acetate or oral desogestrel.

Trioecy in Coccoloba cereifera Schwacke (Polygonaceae), a narrow endemic and threatened tropical species

Trioecy, the co-occurrence of the males, females, and hermaphrodite morphs in natural populations, is a rare and poorly studied breeding system. It is expressed in Coccoloba cereifera, an endemic, and endangered species from the rupestrian fields of Serra do Cipo, southeastern Brazil. Male individuals produce staminate flowers but no fruits. Female individuals produce pistillate flowers and set fruits. Both staminate and pistillate flowers present non-functional organs of the opposite sex that simulate perfect flowers. Hermaphrodite individuals produce two different perfect flowers, each one belonging to distinct individuals, and set fruits. Perfect flowers differ in the amount of pollen produced, in pollen viability, and in some morphological traits. Two Hymenoptera species visited the flowers sporadically. The low natural fructification indicated a limited pollination, while the fruit set recorded in bagged pistillate flowers indicated agamospermy. Female individuals represent more than 40% of the population studied and must be the result of agamospermic seeds.

Biologia reprodutiva de Psychotria carthagenensis (Rubiaceae), espécie distílica de fragmento florestal de mata ciliar, Centro-Oeste do Brasil

Este estudo teve por objetivos analisar a fenologia de floracao, a biologia e a morfologia floral, o sistema e o sucesso reprodutivo dos morfos florais de Psychotria carthagenensis Jacq. O periodo de floracao se estendeu de maio (estacao seca) a janeiro (estacao chuvosa). A floracao dos morfos florais foi sincronizada e o numero medio de inflorescencias, de botoes em pre-antese e de flores abertas por individuos foram semelhantes. A antese das flores de ambos os morfos foi de 12 horas. Houve diferenca significativa no comprimento da corola, dos lobos estigmaticos, das anteras e altura do estilete entre os morfos florais. A viabilidade de polen foi alta entre ambos os morfos. A formacao de frutos ocorreu em polinizacoes intramorfos e intermorfos e nao houve diferenca significativa na producao de frutos e sementes em tratamentos intermorfos. Os atributos florais analisados sugerem que os dois morfos contribuem similarmente para a manutencao da especie na area de estudo.

Sucesso reprodutivo de espécies distílicas de psychotria (rubiaceae) em sub-bosque de floresta atlântica

RESUMO – As especies distilicas Psychotria conjugens, P. hastisepala e P. sessilis (Rubiaceae) sao tipicas de sub-bosques sombreados. Ocorrem no maior fragmento de Floresta Estacional Semidecidual de Vicosa, Minas Gerais, Sudeste brasileiro – a Mata do Paraiso, com 194 ha. A distilia caracteriza-se pela presenca dos morfos florais longistilos (L) e brevistilos (B) em individuos distintos e pela dependencia de polinizacoes intermorfos (L x B ou B x L) para a producao de frutos; e esperada a proporcao equilibrada (isopletia) dos individuos na populacao. Foram objetivos deste trabalho verificar, nas especies citadas, a proporcao dos morfos florais em uma area de 7 ha e a dependencia por polinizadores, testando a incompatibilidade intramorfos (L x L e B x B) por meio de polinizacoes manuais in vivo, a viabilidade dos graos de polen e dimorfismo dos graos entre os morfos florais; e quantificar as producoes de frutos e de sementes por morfo. Os morfos florais das especies se encontram em proporcoes equilibradas. Houve incompatibilidade e a viabilidade dos graos de polen foi alta (> 64%). Verificou-se dimorfismo dos graos, e os maiores diâmetros foram os de B. As producoes de frutos e de sementes (uma ou duas) dos morfos de P. sessilis e de P. conjugens foram semelhantes e, em P. hastisepala, foram maiores em B. Na Mata do Paraiso, as condicoes adequadas, como o habitat, a isopletia e a atuacao de polinizadores, sao fatores que parecem favorecer o sucesso reprodutivo e, consequentemente, a manutencao local das especies estudadas.

PRODUCTIVITY, FRUIT PHYSICOCHEMICAL QUALITY AND DISTINCTIVENESS OF PASSION FRUIT POPULATIONS

A avaliacao da produtividade e da qualidade fisico-quimica apresenta importância, pois permite identificar populacoes superiores. Porem, para lancar material, e necessario seguir os descritores conforme as instrucoes para a execucao dos ensaios de DHE. Desta maneira, objetivou-se avaliar tres populacoes de maracujazeiro-azedo que apresentem caracteristicas de alta produtividade e qualidade fisico-quimica, para lancamento comercial. O experimento foi conduzido na area experimental da Universidade do Estado de Mato Grosso, situada no municipio de Tangara da Serra-MT. O delineamento experimental foi o de blocos completos casualizados, com quatro repeticoes e dez plantas por parcela. As caracteristicas fisico-quimicas foram submetidas a analise de variância e comparadas pelo teste de Tukey. Para avaliar a distinguibilidade, foram utilizados 25 descritores, onde os dados quantitativos foram convertidos em multicategoricos, visando a obter matriz de dissimilaridade. A partir da matriz de dissimilaridade, formaram-se grupos pelo metodo de Tocher e UPGMA. A maior produtividade e o maior numero de frutos foram verificados na cultivar BRS Rubi do Cerrado e na populacao UNEMAT S10. As populacoes e as cultivares apresentaram caracteristicas fisico-quimicas que atendem as qualidades exigidas tanto para o mercado in natura como para a industria. Com base no ensaio de distincao entre genotipos, observou-se que os descritores foram eficientes para a diferenciacao das populacoes. A populacao UNEMAT S10 possui caracteristicas que a distinguem das demais cultivares e populacoes avaliadas, alem de possuir alto desempenho agronomico e, portanto, de poder ser lancada como cultivar comercial.

Reproductive biology of Amasonia obovata Gleason (Laminaceae)

Floral mechanisms that ensure seed production via autogamy are more likely to occur in species growing in environments where pollination is scarce. Amasonia obovata was studied in the State of Mato Grosso-Brazil, from 2009 to 2012, to analyze the morphological and reproductive characteristics, aside from investigating the association of the reproductive success with the pollinator frequency and identity. The flowering and fruiting of A. obovata was concentrated in a period of five months during the rainy season. The dichogamy in flowers of A. obovata is not clearly defined, since the sexual functions were overlapped in the male and female phases. The species is self-compatible and not apomictic. The fruiting percentage obtained by hand self-pollination did not differ from cross-breeding (F = 0.74, P =0.39). In the observations from 2010 to 2012, a hummingbird (Thalurania furcata) legitimate visited 20-100% of the flowers in the male and female phases on different A. obovata plants. Due to the high frequency, this hummingbird was considered the single potential pollinator of the species. These findings show that a limited availability of pollinators may select for floral traits and plant mating strategies that lead to a system of self-fertilization.

Sistema reprodutivo e polinização de Lepidagathis sessilifolia (Pohl) Kameyama ex Wassh. & J.R.I. Wood (Acanthaceae), em remanescente florestal da região sudoeste de Mato Grosso, Brasil

The genus Lepidagathis has pantropical distribution and about 100 species, 16 occurring in Brazil, mostly in the Midwest and Southeast regions. The research was carried out to evaluate in the understory of a forest remnant in Tangara da Serra County - MT to examine the flowering phenology, describe the floral morphology and biology and verify the floral visitors to evaluate the system and reproductive success through hand pollination tests. Lepidagathis sessilifolia shows spiciform and terminal inflorescence with calyx of pink color and corolla whitish-pink. Flowering was restricted from March to April, during the rainy season. Floral senescence occurred after 24 or 48 hours. The viability of pollen grains was high (92.5%). The effective pollinator of L. sessilifolia was Partamona nhambiquara (Apidae - Meliponini). The mixed mating system of the species is characterized by the formation of fruits by agamospermy, self and cross-pollination, spontaneous self-pollination, agamospermy and outcrossing. This flexible reproductive system is advantageous because it ensures the maintenance of species in the study area even in the absence of pollinators.

Morphometry, floral resources and efficiency of natural and artificial pollination in fruit quality in cultivars of sour passion fruit

This study aimed to provide information on the biology and floral resources, stigmatic receptivity and viability of pollen grains during rainy season and dry season, and to evaluate the efficiency of natural and artificial pollination on quality of fruits of eight passion fruit cultivars sourced in Tangará da Serra MT. Five commercial cultivars and three populations of the genetic improvement program of the sour passion fruit from the Universidade do Estado de Mato Grosso were evaluated. The climatic conditions of the rainy season favored the greater development of the floral pieces. The sugar concentration in the nectar presented higher averages in the rainy season, in all cultivars, except for FB 200. The climatic conditions of the dry season favored the replacement of the nectar volume. All cultivars evaluated presented pollen viability and stigmatic receptivity higher than 79% and 90%, respectively. The characteristics of fruit mass and percentage of pulp were better in artificial pollination, and the fruits obtained from natural and artificial pollination in all cultivars evaluated presented physical and chemical characteristics that are within the quality standards desired in the commercialization.

HISTOLOGICAL ANALYSIS AND PERFORMANCE OF SOUR PASSION FRUIT POPULATIONS UNDER DIFFERENT ROOTSTOCKS RESISTANT TO Fusarium spp

The present study aimed to describe histological traits of the graft welding process and evaluate the effect of rootstocks on the physical and chemical traits and the productivity of sour passion fruit populations. The experiment was arranged in a randomized block design, in a 4x2 + 4 factorial scheme (cultivars / canopy x rootstocks), three replicates and nine plants per plot. Commercial cultivars and populations and Passiflora alata and P. nitida rootstocks, in addition to ungrafted plants were used as canopy. Cleft grafting was the grafting type adopted. Traits analyzed such as productivity and physical and chemical quality of fruits were submitted to analysis of variance and compared by the Tukey test. The canopy morphology was evaluated according to descriptors of the Ministry of Agriculture, Livestock and Supply. Grafting was carried out for each graft/rootstock combination for histological analysis. Sections were obtained by freehand cuts, stained and mounted on semi-permanent slides, examined under optical microscope and photomicrographed. Rootstocks affected the early cultivation and reduced productivity and number of fruits of passion fruit populations. Grafting did not affect the quality of fruits or the canopy morphology. Considering the complete culture cycle, P. alata species is able to be used as rootstock. The anatomical study demonstrates the occurrence of better compatibility in the connection of P. edulis tissues on P. alata, compared to grafting on P. nitida.

First record of the genus Tropidia Lindl. (Orchidaceae) for Brazil

The genus Tropidia is composed of ca. 20-30 species distributed in southern Asia, South Pacific islands and northern Australia, and a single species occurring in America, from United States to Ecuador. The first record of the genus Tropidia for Brazil is presented here. It consists of Tropidia polystachya, a species found in the Cerrado biome, Midwestern Brazil. Description, illustration, photos, and distribution map of the species are provided. Additionally, the leaf anatomy of the species was studied and it is very similar to the leaves of other species of Tropidieae described so far. DNA sequences of T. polystachya are presented (plastid matK and rbcL and nuclear ITS) in order to assist future phylogenetic studies with the genus.