Cem Çelik

Community-acquired CTX-M-15-type ESBL-producing Escherichia coli meningitis: a case report and literature review.

In this report, a case of community-acquired acute bacterial meningitis (CA-ABM) caused by CTX-M-15-producing Escherichia coli in an elderly male patient was presented in the light of literature. Cultures of cerebrospinal fluid, blood, ear discharge, and stool samples yielded CTX-M-15-producing E. coli in-vitro, which was resistant to the extended-spectrum cephalosporins and ciprofloxacin and susceptible to imipenem, meropenem and amikacin. Meningitis was treated with parenteral meropenem plus parenteral and intraventricular amikacin administration. Since bacterial meningitis is a life-threatening infection, empiric antibiotic therapy with carbapenem can be started before the culture results are obtained, mainly in areas where the ESBL epidemiology is well known.

A case of chronic suppurative otitis media caused by Kerstersia gyiorum.

Chronic suppurative otitis media is inflammation of the middle ear and mastoid that involves discharge and hearing loss. Kerstersia gyiorum is a member of the Alcaligenaceae family that who could not be treated with classical treatments such as patients with chronic otitis media, neck abscesses. K. gyiorum strain isolated from a patient with chronic suppurative otitis media.

Rotavirus and adenovirus gastroenteritis: time series analysis

This study investigated the effects of changes in weather conditions (monthly average temperature, monthly minimum temperature, monthly average humidity) on rotavirus and adenovirus gastroenteritis frequency and whether there was a seasonal correlation.

Stenotrophomonas maltophilia Infections in Adults: Primary Bacteremia and Pneumonia.

Background: Stenotrophomonas maltophilia is the third most frequent non-fermentative Gram-negative bacilli in nosocomial infections, and usually causes severe infections such as primary bacteremia and pneumonia. Objectives: The current study aimed to compare the demographic and clinical characteristics, microbiological findings and final outcomes of the patients with primary bacteremia and nosocomial pneumonia caused by S. maltophilia. Patients and Methods: The current study retrospectively evaluated patients aged 18 years and above with primary bacteremia and nosocomial pneumonia caused by S. maltophilia from January 2006 to December 2013. Medical records of patients, including reports of clinical microbiology and hospital infection control committee, were evaluated. Results: A total of 71 patients with S. maltophilia nosocomial infections, 35 (49.3%) primary bacteremia and 36 (50.7%) pneumonia, were diagnosed. There were no significant differences in gender, age, and co-morbid diseases, except chronic obstructive pulmonary disease; this infection was significantly higher in patients with pneumonia. A slightly higher 14-day mortality was found in patients with pneumonia, but the difference was not statistically significant. Inappropriate antibiotic use and presence of multiple organ dysfunction syndrome were found as independent risk factors for 14-day mortality in multivariate analysis. Conclusions: A slightly higher mortality in patients with pneumonia, caused by S. maltophilia, was strived to explain by advanced age, higher acute physiology and chronic health evaluation (APACHE II) and sepsis related organ failure assessment (SOFA) score, and also higher inappropriate antibiotic use.

Favorable Outcomes for both Mother and Baby Are Possible in Pregnant Women with Crimean-Congo Hemorrhagic Fever Disease: A Case Series and Literature Review

To assess the impact of Crimean-Congo hemorrhagic fever (CCHF) infection during pregnancy on maternal and fetal outcomes, we present the clinical and laboratory findings and outcomes of 5 pregnant women with CCHF infection as well as fetal outcomes. We also reviewed previously reported cases with CCHF infection in pregnant women. All pregnant women with CCHF infection who had been hospitalized between August 2007 and September 2011 were included. The gestational ages at the time of CCHF infection were 8, 18, 20, 21 and 32 weeks. CCHF infection was acquired during the 1st trimester in only 1 case and resulted in spontaneous abortion. The other 4 pregnant women completely recovered, all reached a healthy full-term gestation and 4 term babies were born. All infants had normal birth weight and were found to be healthy on their first examination and follow-up. In the literature concerning CCHF infection in pregnancy, 8 published articles including case reports or case series and 1 poster presentation including 1 case could be accessed. In conclusion, there is a risk of vertical transmission of CCHF infection, and infections acquired early in gestation had a poor prognosis for the fetus.

Evaluation of the compatibility of Phoenix 100 and Microflex LT MALDI-TOF MS systems in the identification of routinely isolated microorganisms in the clinic microbiology laboratory

Abstract Background: Matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF MS) is a quick, reliable, and efficient system for identifying microorganisms. Many centers that use the Phoenix 100 system today may adopt a MALDI-TOF MS system in the future. Our laboratory recently undertook this pivot. The present study evaluates the reproducibility of species identifications made by the Phoenix 100 and MALDI-TOF MS systems, during a period of transitioning laboratory instrumentation. Methods: Eight hundred and twelve microbial isolates, from aerobic cultures of different clinical samples, were identified simultaneously with Phoenix 100 (Becton Dickinson, Sparks, MD, USA) and a Microflex LT MALDI-TOF MS (Bruker Daltonics, Bremen, Germany) devices. Results: Both systems made identical species assignments for 98.9%, 92.1%, 95.1%, and 93.1% of Gram-negative isolates, catalase-positive Gram-positive cocci isolates, catalase-negative Gram-positive cocci isolates, and Candida isolates, respectively. Conclusions: Identifications made by two instruments commonly used in microbiology laboratories, the Phoenix 100 and the Microflex LT MALDI-TOF MS, are highly consistent.

Klinik örneklerden izole edilen anaerobik bakteriler: Yedi yıllık değerlendirme

Ozet Amac. Anaerob bakteriler onemli patolojilere neden olabilen bakteriler olmasina ragmen kultur ve izolasyonda yasanan zorluklar nedeniyle siklikla gozden kacmaktadirlar. Bu calismada 2006-2013 yillari arasinda Cumhuriyet Universitesi Tip Fakultesi Hastanesi Mikrobiyoloji Laboratuvari’na, anaerob infeksiyon on tanisi ile gonderilen orneklerden izole edilen anaerob bakterilerin, dagilimlarinin incelenmesi amaclanmistir. Yontem. Calismaya cesitli kliniklerden anaeob bakterilerin uretimine uygun sekilde alinarak gonderilen 543 ornek dahil edilmis, ornekler laboratuvar kayitlarindan geriye donuk olarak incelenmistir. Bulgular. Calisilan orneklerin 409’unda (%75,3) ureme gorulmezken, 134 ornekte (%24,6) cesitli anaerob bakteriler uretilmistir. Calismamizda izole ettigimiz anaerob bakteriler arasinda da en fazla ureyen bakterinin Bacteroides spp. (%29,9) oldugu gorulurken, ikinci sirada Peptopstreptococcus spp. (%23,1), ucuncu sirada Propionibacterium spp. (%20,2) izole edilmistir. Sonuc. Anaerob bakterilerin uretilmesi guc, zaman alici ve daha pahali olmasina ragmen klinik orneklerden uygun sekilde alinip gonderildiginde onemli miktarda anaerob bakteri infeksiyon etkeni olarak izole edilebilmektedir. Anaerop bakterilerin klinik orneklerden tanimlanmasi ile anaerop etkenli enfeksiyonlarin daha isabetli bir sekilde tedavi edilebilmesine olanak saglanacagi kanaatindeyiz. Calismamiz sonucunda ortaya koydugumuz yerel verilerin anaerob bakterilere bagli infeksiyonlarin takip ve tedavisinde yarar saglayarak bu konudaki literature katki sunacagini dusunuyoruz. Anahtar sozcukler: Anaerob bakteriler, anaerob infeksiyon, bacteroides spp Abstract Aim. Although anaerobic bacteria are a group of bacteria that can cause significant pathological changes, they are are frequently overlooked due to the difficulties in culturing and isolation. This study aims to investigate the distributions of anaerobic bacteria isolated from the specimens sent to the Microbiology Laboratory of the Cumhuriyet University School of Medicine between the years 2006-2013 based on a preliminary diagnosis of anaerobic infection. Methods. This study includes 543 samples properly collected from various clinics, these samples were retrospectively examined using laboratory records. Results. While there was no growth in 409 (75,3%) of the study samples, various anaerobic bacteria were grown on 134 (24,6%) samples. Our study found the highest growth rate (29,9%) to be in Bacteroides spp. among the anaerobic bacteria isolated in our study, followed by Peptopstreptococcus spp. (23,1%), and Propionibacterium spp. (20,2%). Conclusions. As a result, although growing anaerobic bacteria is difficult, time-consuming and more expensive, it is possible to isolate a significant amount of anaerobic bacteria as infectious agents when clinical samples were properly collected and sent. We consider that describing anaerobic bacteria will allow for a more accurate treatment of such infections. We think that the local data obtained with this study will offer benefits in following and treating infections caused by anaerobic bacteria and contribute to the current literature. Keywords: Anaerobic bacteria, anaerobic infection, bacteroides spp