Uğur Tutar

Synthesis and Biological Evaluation of Novel 1-(4-(Hydroxy(1-oxo-1,3-dihydro-2H-inden-2-ylidene)methyl)phenyl)-3-phenylurea Derivatives

A series of novel phenylurea containing 2‐benzoylindan‐1‐one derivatives 3a – 3j were synthesized from the reaction of phenylurea‐substituted acetophenones 1a – 1j with phthalaldehyde 2 under mild reaction conditions in good yields. All synthesized compounds were characterized by spectroscopic methods. The obtained compounds (3a – 3j) were evaluated for anticancer activity against HeLa and C6 cell lines. Antiproliferative activity was determined by the BrdU proliferation ELISA assay, 3f and 3g were found to be most active compounds. The compounds were also screened for antimicrobial activity and all compounds showed remarkable activity against used microorganisms.

Listeria Standart Suşlarının Zamana Bağlı Biyofilm Oluşturma Kapasiteleri

Mikroorganizmalar dogada cevresel stres kosullarina karsi biyofilm formasyonu gibi cesitli direnc mekanizmalari gelistirmektedirler. Biyofilmler mikrobiyal hucrelerin yuzeylere guclu adhezyonu sonucu olusan sesil organizasyonlardir. Biyofilm ile iliskili hucreler ekzopolisakkarit, protein ve DNA’dan olusan ekstraseluler matriks tarafindan korunmaktadir. Gida endustrisinde, biyofilm olusumu curukcul ve patojenik mikroorganizmalarin gida yada gida yuzeylerine tutunma olasiligindan dolayi hijyen ve guvenlik sebepleri ile istenmemektedir. Gida kokenli patojen mikroorganizmalar, gida endustrisinde her yil onemli ekonomik kayiplara sebep olmaktadirlar. Gida patojenleri arasinda oldukca onemli sayilan, yirminci yuzyilin ikinci yarisinda ortaya cikmis olan Listeria monocytogenes gida, cevresel ve klinik orneklerde yaygin olarak bulunmaktadir. Gram pozitif bir bakteri olan L. monocytogenes insanlarda gida kokenli hastalik olan listeriosis olusumundan sorumludur. Bircok calisma Listeria turlerinin cesitli gidalar ile temasta biyofilm olusturdugunu ve ekstraseluler polimerik bilesikleri urettigini gostermektedir. Biyofilmler antimikrobiyal ajanlara karsi daha direncli olduklari icin Listeria turlerinin farkli yuzeylerde biyofilm olusturma kapasitesi gida endustrisi icin buyuk onem tasimaktadir. Calismamizda Listeria standart suslarinin zamana bagli biyofilm olusturma kapasitelerinin arastirilmasi ve konuyla ilgili literature katki saglanmasi hedeflenmistir. Calisma icin 3 farkli Listeria turune ait 4 standart sus kullanilmistir. Kullanilan kulturler L. monocytogenes ATCC 7644, L. monocytogenes ATCC 19111 , Listeria ivanovii ATCC 19119 ve Listeria innocua 6a33090dur. Biyofilm olusumu 96 kuyucuklu plaklar icerisinde, Christensen ve arkadaslari tarafindan onerilen mikroplak metodu modifiye edilerek arastirilmistir. Calismada, 6, 12 ve 24 saatlik inkubasyon sonrasinda zayif biyofilm olusumu gozlemlenirken, 48 saatlik inkubasyon sonunda butun standart strainlerde orta dereceli biyofilm olusumu tespit edilmistir. Buna bagli olarak, biyofilm olusumu ve zaman arasindaki iliskinin incelenmesi sonucunda, 48 saatlik inkubasyondan sonra biyofilm olusumunda artis gozlemlenmistir.

Evaluation of the compatibility of Phoenix 100 and Microflex LT MALDI-TOF MS systems in the identification of routinely isolated microorganisms in the clinic microbiology laboratory

Abstract Background: Matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF MS) is a quick, reliable, and efficient system for identifying microorganisms. Many centers that use the Phoenix 100 system today may adopt a MALDI-TOF MS system in the future. Our laboratory recently undertook this pivot. The present study evaluates the reproducibility of species identifications made by the Phoenix 100 and MALDI-TOF MS systems, during a period of transitioning laboratory instrumentation. Methods: Eight hundred and twelve microbial isolates, from aerobic cultures of different clinical samples, were identified simultaneously with Phoenix 100 (Becton Dickinson, Sparks, MD, USA) and a Microflex LT MALDI-TOF MS (Bruker Daltonics, Bremen, Germany) devices. Results: Both systems made identical species assignments for 98.9%, 92.1%, 95.1%, and 93.1% of Gram-negative isolates, catalase-positive Gram-positive cocci isolates, catalase-negative Gram-positive cocci isolates, and Candida isolates, respectively. Conclusions: Identifications made by two instruments commonly used in microbiology laboratories, the Phoenix 100 and the Microflex LT MALDI-TOF MS, are highly consistent.