Christian P. Andersen

Ozone exposure and nitrogen deposition lowers root biomass of ponderosa pine in the San Bernardino Mountains, California

Abstract Decreased root biomass in forest trees in response to anthropogenic pollutants is believed to be one of the first steps in forest health degradation. Although decreased root biomass has been observed in controlled experiments, ozone effects on mature tree roots in natural stands has not previously been documented. Here we report standing root biomass of ponderosa pine at three sites in the San Bernardino Mountains distributed along a known, long-term pollution gradient of ozone and nitrogen deposition. Trees at each site were assessed for foliar ozone injury and below-ground attributes, in addition to other environmental factors known to influence root growth. During the period of peak root growth in the spring, root biomass at the least polluted site was 6–14 times greater than that observed at the most polluted site. Known differences in climatic and edaphic factors among the sites potentially contributing to the observed response were discounted as primary contributors to the response since in most cases the site differences would have driven the patterns of root growth in the opposite direction to that observed. Differences in biotic competitive interactions, also known to affect root growth, did not explain the observed pattern for the same reason. The data suggests that elevated ozone, high nitrogen deposition, and possibly other contributing factors such as soil acidification are primarily responsible for lowering root biomass in ponderosa pine stands in the San Bernardino Mountains.

Stress Interactions and mycorrhizal plant response: Understanding carbon allocation priorities

In this paper, a framework is presented for studying responses of mycorrhiza to external stresses, including possible feedback effects which are likely to occur. The authors review recent literature linking carbon allocation and host/fungal response under natural and anthropogenic stress, and present a conceptual model to discuss how carbon may be involved in singular and multiple stress interactions of mycorrhizal seedlings. Due to an integral integral role in metabollic processes, characterizing carbon allocation in controlled laboratory environments could be useful for understanding host/fungal responses to a variety of natural and anthropogenic stresses. Carbon allocation at the whole-plant level reflects an integrated response which links photosynthesis to growth and maintenance processes. A root-mycocosm system is described which permits spatial separation of a portion of extramatrical hyphae growing in association with seedling roots. Using this system, it is shown that root/hyphal respiratory release of pulse-labeled 14C follows a sigmoidal pattern, with typical lag, exponential and saturation phases. Total respiratory release of 14C per mg root and the fraction respired of total 14C allocated to the root is greater in ponderosa pine inoculated with Hebeloma crustuliniforme than in noninoculated controls. Results illustrate the nature of information than can be obtained using this system. Current projects using the mycocosms include characterizing the dynamics of carbon allocation under ozone stress, and following the fate of organic pollutants. The authors believe that the system could be used to differentiate fungal- and host-mediated responses to a large number of other stresses and to study a variety of physiological processes in mycorrhizal plants.

Ecological and water quality consequences of nutrient addition for salmon restoration in the Pacific Northwest

Salmon runs have declined over the past two centuries in the Pacific Northwest region of North America. Reduced inputs of salmon-derived organic matter and nutrients (SDN) may limit freshwater production and thus establish a negative feedback loop affecting future generations of fish. Restoration efforts use the rationale of declining SDN to justify artificial nutrient additions, with the goal of reversing salmon decline. The forms of nutrient addition include introducing salmon carcasses, carcass analogs (processed fish cakes), or inorganic fertilizers. While evidence suggests that fish and wildlife may benefit from increases in food availability as a result of carcass additions, stream ecosystems vary in their ability to use nutrients to benefit salmon. Moreover, the practice may introduce excess nutrients, disease, and toxic substances to streams that may already exceed proposed water quality standards. Restoration efforts involving nutrient addition must balance the potential benefits of increased foo...

Belowground effects of enhanced tropospheric ozone and drought in a beech/spruce forest (Fagus sylvatica L./Picea abies [L.] Karst)

The effects of experimentally elevated O(3) on soil respiration rates, standing fine-root biomass, fine-root production and delta(13)C signature of newly produced fine roots were investigated in an adult European beech/Norway spruce forest in Germany during two subsequent years with contrasting rainfall patterns. During humid 2002, soil respiration rate was enhanced under elevated O(3) under beech and spruce, and was related to O(3)-stimulated fine-root production only in beech. During dry 2003, the stimulating effect of O(3) on soil respiration rate vanished under spruce, which was correlated with decreased fine-root production in spruce under drought, irrespective of the O(3) regime. delta(13)C signature of newly formed fine-roots was consistent with the differing g(s) of beech and spruce, and indicated stomatal limitation by O(3) in beech and by drought in spruce. Our study showed that drought can override the stimulating O(3) effects on fine-root dynamics and soil respiration in mature beech and spruce forests.

Ozone and natural systems: understanding exposure, response, and risk

Research aimed at understanding the response of plants to ozone has been conducted for over four decades but little of it has addressed intact natural systems. Even so, there is sufficient scientific information at this time to support air quality standards that will protect natural terrestrial ecosystems from ozone. What is unknown is the risk associated with continued exposure of natural systems, including both above- and below-ground components, in combination with other stresses including changing temperature and precipitation, elevated carbon dioxide, pests and pathogens, invasive species, and other activities that may fragment the landscape. Research to support an assessment of the ecological risk associated with ozone as it exists, in a milieu of stresses, must include endpoints beyond those addressed in the past, primarily productivity and species composition. To estimate the risk to society of ozone impacts on natural systems, endpoints such as the integrity of soil food webs, the quantity and quality of water supplied from terrestrial ecosystems, wildlife and recreational values, and the transfer and fate of carbon, nutrients, and water within the systems must be quantified. Not only will this research provide the basis for a sound estimate of risk, but also it will improve our understanding of fundamental ecosystem processes.

Differences in above- and below-ground responses to ozone between two populations of a perennial grass

Our study examined the influence of elevated ozone levels on the growth and mycorrhizal colonization of two populations of Elymus glaucus L. (blue wildrye). We hypothesized that ozone would reduce carbon availability to the plants, particularly below ground, and would affect mycorrhizal colonization. Because of the wide geographic range of E. glaucus, two populations of plants were selected from areas of contrasting ozone histories to examine intraspecies variation in response to ozone. Two populations of E. glaucus (southern California versus northern California) exposed in a factorial experiment involving ozone, mycorrhizal inoculation with Glomus intraradices Schenck and Smith, and plant source population. Ozone had a subtle effect on leaf area and number of tillers but did not affect overall root:shoot ratio in either population. The impact of ozone on above-ground growth characteristics was most pronounced in the southern population that came from a high-ozone environment, while below-ground responses such as reduced arbuscular colonization was most pronounced in the northern population which originated in a low-ozone environment. Further analysis of soil characteristics from the northern population of plants revealed a significant reduction in active soil bacterial biomass and an increase in total fungi per gram dry weight soil, suggesting a possible role for ozone in altering soil processes. Whether or not population differences in response to ozone were due to genetic shifts resulting from prior ozone remains to be determined. However, these subtle but important differences in population response to ozone above- and below-ground have significant implications in any attempt to generalize plant response, even within a species. Future research efforts need to include better characterization of intraspecific variation in response to ozone as well as possible adaptive strategies that may result from chronic ozone exposure.

The Physiological Basis of Differential Plant Sensitivity to Changes in Atmospheric Quality

During the next several decades vegetation will continue to be exposed to a wide variety of pollutants, although the types of compounds, their concentrations, and their spatial patterns may change. Some are distributed globally, whereas others are distributed regionally or locally. These airborne pollutants can have either a direct impact on the plant foliage or act indirectly through deposition onto the soil and subsequent uptake by roots. These effects can range from subtle modifications of cellular biochemistry and whole-plant physiology (e.g., carbon allocation) to overt foliar injury and effects on plant growth, yield, and/or reproduction.

Germination and early plant development of ten plant species exposed to TiO2 and CeO2 nanoparticles

Ten agronomic plant species were exposed to different concentrations of nano-titanium dioxide (nTiO2 ) or nano-cerium oxide (nCeO2 ) (0 μg/mL, 250 μg/mL, 500 μg/mL, and 1000 μg/mL) to examine potential effects on germination and early seedling development. The authors modified a standard test protocol developed for soluble chemicals (OPPTS 850.4200) to determine if such an approach might be useful for screening engineered nanomaterials (ENMs) and whether there were differences in response across a range of commercially important plant species to 2 common metal oxide ENMs. Eight of 10 species responded to nTiO2 , and 5 species responded to nCeO2 . Overall, it appeared that early root growth may be a more sensitive indicator of potential effects from ENM exposure than germination. The observed effects did not always relate to the exposure concentration, indicating that mass-based concentration may not fully explain the developmental effects of these 2 ENMs. The results suggest that nTiO2 and nCeO2 have different effects on early plant growth of agronomic species, with unknown effects at later stages of the life cycle. In addition, standard germination tests, which are commonly used for toxicity screening of new materials, may not detect the subtle but potentially more important changes associated with early growth and development in terrestrial plants. Environ Toxicol Chem 2016;35:2223-2229. Published 2016 Wiley Periodicals Inc. on behalf of SETAC. This article is a US Government work and, as such, is in the public domain in the United States of America.

Germination and early plant development of ten plant species exposed to titanium dioxide and cerium oxide nanoparticles.

Ten agronomic plant species were exposed to different concentrations of nano-titanium dioxide (nTiO2 ) or nano-cerium oxide (nCeO2 ) (0 μg/mL, 250 μg/mL, 500 μg/mL, and 1000 μg/mL) to examine potential effects on germination and early seedling development. The authors modified a standard test protocol developed for soluble chemicals (OPPTS 850.4200) to determine if such an approach might be useful for screening engineered nanomaterials (ENMs) and whether there were differences in response across a range of commercially important plant species to 2 common metal oxide ENMs. Eight of 10 species responded to nTiO2 , and 5 species responded to nCeO2 . Overall, it appeared that early root growth may be a more sensitive indicator of potential effects from ENM exposure than germination. The observed effects did not always relate to the exposure concentration, indicating that mass-based concentration may not fully explain the developmental effects of these 2 ENMs. The results suggest that nTiO2 and nCeO2 have different effects on early plant growth of agronomic species, with unknown effects at later stages of the life cycle. In addition, standard germination tests, which are commonly used for toxicity screening of new materials, may not detect the subtle but potentially more important changes associated with early growth and development in terrestrial plants. Environ Toxicol Chem 2016;35:2223-2229. Published 2016 Wiley Periodicals Inc. on behalf of SETAC. This article is a US Government work and, as such, is in the public domain in the United States of America.

Phenotypic and genomic responses to titanium dioxide and cerium oxide nanoparticles in Arabidopsis germinants

The effects of exposure to nanoparticles of titanium dioxide (nano-titanium) and cerium oxide (nano-cerium) on gene expression and growth in Arabidopsis thaliana germinants were studied by using microarrays and quantitative real-time polymerase chain reaction (qPCR), and by evaluating germinant phenotypic plasticity. Exposure to 12 d of either nano-titania or nano-ceria altered the regulation of 204 and 142 genes, respectively. Genes induced by the nanoparticles mainly include ontology groups annotated as stimuli responsive, including both abiotic (oxidative stress, salt stress, water transport) and biotic (respiratory burst as a defense against pathogens) stimuli. Further analysis of the differentially expressed genes indicates that both nanoparticles affected a range of metabolic processes (deoxyribonucleic acid [DNA] metabolism, hormone metabolism, tetrapyrrole synthesis, and photosynthesis). Individual exposures to the nanoparticles increased percentages of seeds with emergent radicles, early development of hypocotyls and cotyledons, and those with fully grown leaves. Although there were distinct differences between the nanoparticles in their affect on molecular mechanisms attributable to enhancing germinant growth, both particles altered similar suites of genes related to various pathways and processes related to enhanced growth.