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Dive into the research topics where Christie Kovar is active.

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Featured researches published by Christie Kovar.


Nature | 2011

A high-resolution map of human evolutionary constraint using 29 mammals

Kerstin Lindblad-Toh; Manuel Garber; Or Zuk; Michael F. Lin; Brian J. Parker; Stefan Washietl; Pouya Kheradpour; Jason Ernst; Gregory Jordan; Evan Mauceli; Lucas D. Ward; Craig B. Lowe; Alisha K. Holloway; Michele Clamp; Sante Gnerre; Jessica Alföldi; Kathryn Beal; Jean Chang; Hiram Clawson; James Cuff; Federica Di Palma; Stephen Fitzgerald; Paul Flicek; Mitchell Guttman; Melissa J. Hubisz; David B. Jaffe; Irwin Jungreis; W. James Kent; Dennis Kostka; Marcia Lara

The comparison of related genomes has emerged as a powerful lens for genome interpretation. Here we report the sequencing and comparative analysis of 29 eutherian genomes. We confirm that at least 5.5% of the human genome has undergone purifying selection, and locate constrained elements covering ∼4.2% of the genome. We use evolutionary signatures and comparisons with experimental data sets to suggest candidate functions for ∼60% of constrained bases. These elements reveal a small number of new coding exons, candidate stop codon readthrough events and over 10,000 regions of overlapping synonymous constraint within protein-coding exons. We find 220 candidate RNA structural families, and nearly a million elements overlapping potential promoter, enhancer and insulator regions. We report specific amino acid residues that have undergone positive selection, 280,000 non-coding elements exapted from mobile elements and more than 1,000 primate- and human-accelerated elements. Overlap with disease-associated variants indicates that our findings will be relevant for studies of human biology, health and disease.


Nature | 2012

Butterfly genome reveals promiscuous exchange of mimicry adaptations among species

Kanchon K. Dasmahapatra; James R. Walters; Adriana D. Briscoe; John W. Davey; Annabel Whibley; Nicola J. Nadeau; Aleksey V. Zimin; Daniel S.T. Hughes; Laura Ferguson; Simon H. Martin; Camilo Salazar; James J. Lewis; Sebastian Adler; Seung-Joon Ahn; Dean A. Baker; Simon W. Baxter; Nicola Chamberlain; Ritika Chauhan; Brian A. Counterman; Tamas Dalmay; Lawrence E. Gilbert; Karl H.J. Gordon; David G. Heckel; Heather M. Hines; Katharina Hoff; Peter W. H. Holland; Emmanuelle Jacquin-Joly; Francis M. Jiggins; Robert T. Jones; Durrell D. Kapan

The evolutionary importance of hybridization and introgression has long been debated. Hybrids are usually rare and unfit, but even infrequent hybridization can aid adaptation by transferring beneficial traits between species. Here we use genomic tools to investigate introgression in Heliconius, a rapidly radiating genus of neotropical butterflies widely used in studies of ecology, behaviour, mimicry and speciation. We sequenced the genome of Heliconius melpomene and compared it with other taxa to investigate chromosomal evolution in Lepidoptera and gene flow among multiple Heliconius species and races. Among 12,669 predicted genes, biologically important expansions of families of chemosensory and Hox genes are particularly noteworthy. Chromosomal organization has remained broadly conserved since the Cretaceous period, when butterflies split from the Bombyx (silkmoth) lineage. Using genomic resequencing, we show hybrid exchange of genes between three co-mimics, Heliconius melpomene, Heliconius timareta and Heliconius elevatus, especially at two genomic regions that control mimicry pattern. We infer that closely related Heliconius species exchange protective colour-pattern genes promiscuously, implying that hybridization has an important role in adaptive radiation.


Science | 2010

A catalog of reference genomes from the human microbiome.

Karen E. Nelson; George M. Weinstock; Sarah K. Highlander; Kim C. Worley; Heather Huot Creasy; Jennifer R. Wortman; Douglas B. Rusch; Makedonka Mitreva; Erica Sodergren; Asif T. Chinwalla; Michael Feldgarden; Dirk Gevers; Brian J. Haas; Ramana Madupu; Doyle V. Ward; Bruce Birren; Richard A. Gibbs; Barbara A. Methé; Joseph F. Petrosino; Robert L. Strausberg; Granger Sutton; Owen White; Richard Wilson; Scott Durkin; Michelle G. Giglio; Sharvari Gujja; Clint Howarth; Chinnappa D. Kodira; Nikos C. Kyrpides; Teena Mehta

News from the Inner Tube of Life A major initiative by the U.S. National Institutes of Health to sequence 900 genomes of microorganisms that live on the surfaces and orifices of the human body has established standardized protocols and methods for such large-scale reference sequencing. By combining previously accumulated data with new data, Nelson et al. (p. 994) present an initial analysis of 178 bacterial genomes. The sampling so far barely scratches the surface of the microbial diversity found on humans, but the work provides an important baseline for future analyses. Standardized protocols and methods are being established for large-scale sequencing of the microorganisms living on humans. The human microbiome refers to the community of microorganisms, including prokaryotes, viruses, and microbial eukaryotes, that populate the human body. The National Institutes of Health launched an initiative that focuses on describing the diversity of microbial species that are associated with health and disease. The first phase of this initiative includes the sequencing of hundreds of microbial reference genomes, coupled to metagenomic sequencing from multiple body sites. Here we present results from an initial reference genome sequencing of 178 microbial genomes. From 547,968 predicted polypeptides that correspond to the gene complement of these strains, previously unidentified (“novel”) polypeptides that had both unmasked sequence length greater than 100 amino acids and no BLASTP match to any nonreference entry in the nonredundant subset were defined. This analysis resulted in a set of 30,867 polypeptides, of which 29,987 (~97%) were unique. In addition, this set of microbial genomes allows for ~40% of random sequences from the microbiome of the gastrointestinal tract to be associated with organisms based on the match criteria used. Insights into pan-genome analysis suggest that we are still far from saturating microbial species genetic data sets. In addition, the associated metrics and standards used by our group for quality assurance are presented.


Nature | 2011

Comparative and demographic analysis of orang-utan genomes

Devin P. Locke; LaDeana W. Hillier; Wesley C. Warren; Kim C. Worley; Lynne V. Nazareth; Donna M. Muzny; Shiaw-Pyng Yang; Zhengyuan Wang; Asif T. Chinwalla; Patrick Minx; Makedonka Mitreva; Lisa Cook; Kim D. Delehaunty; Catrina C. Fronick; Heather K. Schmidt; Lucinda A. Fulton; Robert S. Fulton; Joanne O. Nelson; Vincent Magrini; Craig S. Pohl; Tina Graves; Chris Markovic; Andy Cree; Huyen Dinh; Jennifer Hume; Christie Kovar; Gerald Fowler; Gerton Lunter; Stephen Meader; Andreas Heger

‘Orang-utan’ is derived from a Malay term meaning ‘man of the forest’ and aptly describes the southeast Asian great apes native to Sumatra and Borneo. The orang-utan species, Pongo abelii (Sumatran) and Pongo pygmaeus (Bornean), are the most phylogenetically distant great apes from humans, thereby providing an informative perspective on hominid evolution. Here we present a Sumatran orang-utan draft genome assembly and short read sequence data from five Sumatran and five Bornean orang-utan genomes. Our analyses reveal that, compared to other primates, the orang-utan genome has many unique features. Structural evolution of the orang-utan genome has proceeded much more slowly than other great apes, evidenced by fewer rearrangements, less segmental duplication, a lower rate of gene family turnover and surprisingly quiescent Alu repeats, which have played a major role in restructuring other primate genomes. We also describe a primate polymorphic neocentromere, found in both Pongo species, emphasizing the gradual evolution of orang-utan genome structure. Orang-utans have extremely low energy usage for a eutherian mammal, far lower than their hominid relatives. Adding their genome to the repertoire of sequenced primates illuminates new signals of positive selection in several pathways including glycolipid metabolism. From the population perspective, both Pongo species are deeply diverse; however, Sumatran individuals possess greater diversity than their Bornean counterparts, and more species-specific variation. Our estimate of Bornean/Sumatran speciation time, 400,000 years ago, is more recent than most previous studies and underscores the complexity of the orang-utan speciation process. Despite a smaller modern census population size, the Sumatran effective population size (Ne) expanded exponentially relative to the ancestral Ne after the split, while Bornean Ne declined over the same period. Overall, the resources and analyses presented here offer new opportunities in evolutionary genomics, insights into hominid biology, and an extensive database of variation for conservation efforts.


BMC Microbiology | 2007

Subtle genetic changes enhance virulence of methicillin resistant and sensitive Staphylococcus aureus

Sarah K. Highlander; Kristina G. Hulten; Xiang Qin; Huaiyang Jiang; Shailaja Yerrapragada; Edward O. Mason; Yue Shang; Tiffany M. Williams; Régine M Fortunov; Yamei Liu; Okezie Igboeli; Joseph F. Petrosino; Madhan R. Tirumalai; Akif Uzman; George E. Fox; Ana Maria Cardenas; Donna M. Muzny; Lisa Hemphill; Yan Ding; Shannon Dugan; Peter R Blyth; Christian Buhay; Huyen Dinh; Alicia Hawes; Michael Holder; Christie Kovar; Sandra L. Lee; Wen Liu; Lynne V. Nazareth; Qiaoyan Wang

BackgroundCommunity acquired (CA) methicillin-resistant Staphylococcus aureus (MRSA) increasingly causes disease worldwide. USA300 has emerged as the predominant clone causing superficial and invasive infections in children and adults in the USA. Epidemiological studies suggest that USA300 is more virulent than other CA-MRSA. The genetic determinants that render virulence and dominance to USA300 remain unclear.ResultsWe sequenced the genomes of two pediatric USA300 isolates: one CA-MRSA and one CA-methicillin susceptible (MSSA), isolated at Texas Childrens Hospital in Houston. DNA sequencing was performed by Sanger dideoxy whole genome shotgun (WGS) and 454 Life Sciences pyrosequencing strategies. The sequence of the USA300 MRSA strain was rigorously annotated. In USA300-MRSA 2658 chromosomal open reading frames were predicted and 3.1 and 27 kilobase (kb) plasmids were identified. USA300-MSSA contained a 20 kb plasmid with some homology to the 27 kb plasmid found in USA300-MRSA. Two regions found in US300-MRSA were absent in USA300-MSSA. One of these carried the arginine deiminase operon that appears to have been acquired from S. epidermidis. The USA300 sequence was aligned with other sequenced S. aureus genomes and regions unique to USA300 MRSA were identified.ConclusionUSA300-MRSA is highly similar to other MRSA strains based on whole genome alignments and gene content, indicating that the differences in pathogenesis are due to subtle changes rather than to large-scale acquisition of virulence factor genes. The USA300 Houston isolate differs from another sequenced USA300 strain isolate, derived from a patient in San Francisco, in plasmid content and a number of sequence polymorphisms. Such differences will provide new insights into the evolution of pathogens.


BMC Genomics | 2014

Finding the missing honey bee genes: Lessons learned from a genome upgrade

Christine G. Elsik; Kim C. Worley; Anna K. Bennett; Martin Beye; Francisco Camara; Christopher P. Childers; Dirk C. de Graaf; Griet Debyser; Jixin Deng; Bart Devreese; Eran Elhaik; Jay D. Evans; Leonard J. Foster; Dan Graur; Roderic Guigó; Katharina Hoff; Michael Holder; Matthew E. Hudson; Greg J. Hunt; Huaiyang Jiang; Vandita Joshi; Radhika S. Khetani; Peter Kosarev; Christie Kovar; Jian Ma; Ryszard Maleszka; Robin F. A. Moritz; Monica Munoz-Torres; Terence Murphy; Donna M. Muzny

BackgroundThe first generation of genome sequence assemblies and annotations have had a significant impact upon our understanding of the biology of the sequenced species, the phylogenetic relationships among species, the study of populations within and across species, and have informed the biology of humans. As only a few Metazoan genomes are approaching finished quality (human, mouse, fly and worm), there is room for improvement of most genome assemblies. The honey bee (Apis mellifera) genome, published in 2006, was noted for its bimodal GC content distribution that affected the quality of the assembly in some regions and for fewer genes in the initial gene set (OGSv1.0) compared to what would be expected based on other sequenced insect genomes.ResultsHere, we report an improved honey bee genome assembly (Amel_4.5) with a new gene annotation set (OGSv3.2), and show that the honey bee genome contains a number of genes similar to that of other insect genomes, contrary to what was suggested in OGSv1.0. The new genome assembly is more contiguous and complete and the new gene set includes ~5000 more protein-coding genes, 50% more than previously reported. About 1/6 of the additional genes were due to improvements to the assembly, and the remaining were inferred based on new RNAseq and protein data.ConclusionsLessons learned from this genome upgrade have important implications for future genome sequencing projects. Furthermore, the improvements significantly enhance genomic resources for the honey bee, a key model for social behavior and essential to global ecology through pollination.


PLOS ONE | 2012

Evaluation of 16s rDNA-based community profiling for human microbiome research

Doyle V. Ward; Dirk Gevers; Georgia Giannoukos; Ashlee M. Earl; Barbara A. Methé; Erica Sodergren; Michael Feldgarden; Dawn Ciulla; Diana Tabbaa; Cesar Arze; Elizabeth L. Appelbaum; Leigh Aird; Scott Anderson; Tulin Ayvaz; Edward A. Belter; Monika Bihan; Toby Bloom; Jonathan Crabtree; Laura Courtney; Lynn K. Carmichael; David J. Dooling; Rachel L. Erlich; Candace N. Farmer; Lucinda Fulton; Robert S. Fulton; Hongyu Gao; John Gill; Brian J. Haas; Lisa Hemphill; Otis Hall

The Human Microbiome Project will establish a reference data set for analysis of the microbiome of healthy adults by surveying multiple body sites from 300 people and generating data from over 12,000 samples. To characterize these samples, the participating sequencing centers evaluated and adopted 16S rDNA community profiling protocols for ABI 3730 and 454 FLX Titanium sequencing. In the course of establishing protocols, we examined the performance and error characteristics of each technology, and the relationship of sequence error to the utility of 16S rDNA regions for classification- and OTU-based analysis of community structure. The data production protocols used for this work are those used by the participating centers to produce 16S rDNA sequence for the Human Microbiome Project. Thus, these results can be informative for interpreting the large body of clinical 16S rDNA data produced for this project.


Science | 2014

The sheep genome illuminates biology of the rumen and lipid metabolism

Yu Jiang; Min Xie; Wenbin Chen; Richard Talbot; J. F. Maddox; Thomas Faraut; Chunhua Wu; Donna M. Muzny; Yuxiang Li; Wenguang Zhang; Jo-Ann L. Stanton; Rudiger Brauning; Wesley C. Barris; Thibaut Hourlier; Bronwen Aken; Stephen M. J. Searle; David L. Adelson; Chao Bian; Graham R. Cam; Yulin Chen; Shifeng Cheng; Udaya DeSilva; Karen Dixen; Yang Dong; Guangyi Fan; Ian R. Franklin; Shaoyin Fu; Pablo Fuentes-Utrilla; Rui Guan; Margaret A. Highland

A genome for ewe and ewe Sheep-specific genetic changes underlie differences in lipid metabolism between sheep and other mammals, and may have contributed to the production of wool. Jiang et al. sequenced the genome of two Texel sheep, a breed that produces high-value meat, milk, and wool. The genome information will provide an important resource for livestock production and aid in the understanding of mammalian evolution. Science, this issue p. 1168 A genomic analysis of sheep explains specializations in digestive system physiology and wool production. Sheep (Ovis aries) are a major source of meat, milk, and fiber in the form of wool and represent a distinct class of animals that have a specialized digestive organ, the rumen, that carries out the initial digestion of plant material. We have developed and analyzed a high-quality reference sheep genome and transcriptomes from 40 different tissues. We identified highly expressed genes encoding keratin cross-linking proteins associated with rumen evolution. We also identified genes involved in lipid metabolism that had been amplified and/or had altered tissue expression patterns. This may be in response to changes in the barrier lipids of the skin, an interaction between lipid metabolism and wool synthesis, and an increased role of volatile fatty acids in ruminants compared with nonruminant animals.


Genome Biology | 2010

Whole exome capture in solution with 3 Gbp of data.

Matthew N. Bainbridge; Min Wang; Daniel Burgess; Christie Kovar; Matthew Rodesch; Mark D'Ascenzo; Jacob Kitzman; Yuan Qing Wu; Irene Newsham; Todd Richmond; Jeffrey A. Jeddeloh; Donna M. Muzny; Thomas J. Albert; Richard A. Gibbs

We have developed a solution-based method for targeted DNA capture-sequencing that is directed to the complete human exome. Using this approach allows the discovery of greater than 95% of all expected heterozygous singe base variants, requires as little as 3 Gbp of raw sequence data and constitutes an effective tool for identifying rare coding alleles in large scale genomic studies.


Nature Genetics | 2015

Convergent evolution of the genomes of marine mammals

Andrew D. Foote; Yue Liu; Gregg W.C. Thomas; Tomáš Vinař; Jessica Alföldi; Jixin Deng; Shannon Dugan; Cornelis E van Elk; Margaret E Hunter; Vandita Joshi; Ziad Khan; Christie Kovar; Sandra L. Lee; Kerstin Lindblad-Toh; Annalaura Mancia; Rasmus Nielsen; Xiang Qin; Jiaxin Qu; Brian J. Raney; Nagarjun Vijay; Jochen B. W. Wolf; Matthew W. Hahn; Donna M. Muzny; Kim C. Worley; M. Thomas P. Gilbert; Richard A. Gibbs

Marine mammals from different mammalian orders share several phenotypic traits adapted to the aquatic environment and therefore represent a classic example of convergent evolution. To investigate convergent evolution at the genomic level, we sequenced and performed de novo assembly of the genomes of three species of marine mammals (the killer whale, walrus and manatee) from three mammalian orders that share independently evolved phenotypic adaptations to a marine existence. Our comparative genomic analyses found that convergent amino acid substitutions were widespread throughout the genome and that a subset of these substitutions were in genes evolving under positive selection and putatively associated with a marine phenotype. However, we found higher levels of convergent amino acid substitutions in a control set of terrestrial sister taxa to the marine mammals. Our results suggest that, whereas convergent molecular evolution is relatively common, adaptive molecular convergence linked to phenotypic convergence is comparatively rare.

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Donna M. Muzny

Baylor College of Medicine

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Richard A. Gibbs

Baylor College of Medicine

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Huaiyang Jiang

Baylor College of Medicine

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Huyen Dinh

Baylor College of Medicine

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Kim C. Worley

Baylor College of Medicine

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Christian Buhay

Baylor College of Medicine

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George M. Weinstock

Washington University in St. Louis

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Jiaxin Qu

Baylor College of Medicine

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Lynne V. Nazareth

Baylor College of Medicine

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