Cláudio Baptista de Carvalho

ANION GAP NO SANGUE VENOSO EM EQUINOS

SUMMARY The influence of venous blood in the determination ofthe anion gap was studied in 50 adult clinically normal horses in Santa Maria - Brazil. The results (mEq/1) were: Na - 140 ± 2.0; K - 42 ± 0.5; Cl -102 ± 24 and HCO3 - 26.9 ± 2.0. The results were similar but slightly different from those of other authors, but without significam difference, although these authors have used arterial blood. It was concluded that the venous blood can replace the arterial blood in order to determinate the anion gap in horses.

DETERMINAÇÃO DOS VALORES MÉDIOS DAS ENZIMAS AST, DHL, gGT E FAS NO SORO DE EQUINOS SADIOS EM SANTA MARIA, RS

Foram usados 50 equinos sadios provenientes do Batalhao de Policia Montada da Brigada Militar em Santa Maria, RS, sendo 43 machos e 7 femeas com idade variadas a partir de 3 anos. Foram colhidos 10ml de sangue da jugular para determinacao dos valores da atividade serica das enzimas aspartato-aminotransferase (AST), desidrogenase latica (DHL), gama-glutamiltransferase (gGT) e fosfatase alcalina serica (FAS). Os resultados encontrados para AST foi de 101 - 190U/I com media de 130UI; DHL foi de 100 - 421 U/l com media de 182U/I; gGT foi de 2 - 27U/I com media de 6.5U/I e FAS foi de 103 - 335U/I com media de 190U/I. A partir de outubro/1992 estes valores passaram a ser referencia no laboratorio de Patologia Clinica do Hospital de Clinicas Veterinarias da Universidade Federal de Santa Maria.

Cardiac markers in five different breeds of rabbits (Oryctolagus cuniculus Linnaeus, 1758) used for cardiovascular research

Biomarcadores cardiacos tem sido avaliados em coelhos para avaliacao clinica e experimental das doencas cardiacas. Entretanto diferentes testes laboratoriais tem sido utilizados e relatados, sem uma confluencia de resultados. Os objetivos deste estudo foram verificar os efeitos de diferentes racas de coelhos sobre as concentracoes sericas de ANP, CRP e ACE, alem de estabelecer intervalor de referencia para estes biomarcadores em uma populacao de coelhos saudaveis. Foram utilizados noventa e sete coelhos de cinco diferentes racas. Os exames foram realizados pela metodologia de ELISA, por meio de kits comerciais especificos. Os resultados foram analisados estatisticamente os testes de ANOVA e Tukey (p<0,05), media aritmetica, intervalo de referencia da media e desvio padrao. Um efeito significativo da raca foi observado sobre as variaveis estudadas, indicando diferentes intervalos de referencia entre as racas para cada biomarcador. Em conclusao, este estudo demonstrou que a raca e uma variavel fisiologica importante que influencia os valores normais destes biomarcadores em coelhos saudaveis.

Oxigenação extracorpórea por membrana em cães sem auxílio circulatório

In humans, arteriovenous extracorporeal membrane oxygenation (AV-ECMO) is a life support technique capable to assume in part or totally lungs functions, when these are not able to promote appropriated hematosis. To know the viability of this method in dogs (Canis familiaris), the present study applied AV-ECMO in ten adult mixed-breed dogs, with expected three hours of duration to each experiment. In Group 1 (n=4), mechanical ventilation and dopamine continuous infusion were associated with the extracorporeal support. In Group 2 (n=6), any other measure of intensive care was used concomitantly AV-ECMO. Blood gas analysis and lactate levels were evaluated every 10 minutes and one hour, respectively, from arterial blood samples. Inotropic support improved the blood flow through the circuit promoting a better tissue perfusion with oxygenated blood, suggested by the lactate and mean arterial pressure levels measured. Mechanical ventilation used simultaneously with AV-ECMO was able to further improve levels of blood gases. Therefore, if applied along intensive care support, AV-ECMO is a viable technique for use in dogs with respiratory failure refractory to conventional treatments.

Hemograma de bovinos portadores do "Mal-da-Ronca" na região norte do estado do Rio de Janeiro

Na avaliacao hematologica de 58 bovinos mesticos de zebu, com predominância de mesticos da raca nelore, na regiao Nortedo estado do Rio de Janeiro, divididos em dois grupos de 29 animais cada, sendo um de bovinos sadios e outro de bovinosdoentes (Mal da Ronca), constatou-se que o eritrograma dos animais observados nao apresentou alteracoes. O leucogramademonstrou diferenca significativa entre os grupos, segundo o Teste de Wilcoxon (p:o; 0,05) em relacao aos niveis de leucocitose linfocitos.

Variação circadiana dos leucócitos, fibrinogênio e plaquetas no sangue de cães submetidos a processo inflamatório, tratados com escina

To analyse the circadian variations of the leucocyte, fibrinogen and platelet 20 mongrel male dogs were used divided into two groups of ten animals. The first group was exposed to an inflamatory process induced by the administration of turpentine and after 24 hours the animals were treated with Aesein 1.3mg/kg from 12 to 12 hours. The second group was only exposed to the inflamatory process. Blood samples were performed in all the animals at 0,4am, 8am, 12am, 4pm, 8pm and 12pm hours of the day to examine the parameters. Aescin has determined monocytose at 0,8am, 12am, 4pm and 12pm; linfopenia at 4pm and 12pm; leucocitosis at 0,8pm and 12 pm, as well as an increase in the amount of fibrinogen at 4pm and 12pm; and a decrease in the platelets during all day. Aescin determines unstable circadian variations in the number of leucocyte, platelet and in the amount of fibrinogen in dogs with inflamatory process.

AÇÃO DA ESCINA SOBRE A VARIAÇÃO CIRCADIANA DOS LEUCÓCITOS, FIBRINOGÊNIO E PLAQUETAS EM SANGUE DE CÃES SADIOS

Twenty mongrel male dogs were used to anlyse the circadian variations of leucocyte, fibrinogen and platelet values. They were divided into two groups of ten animals. The first group was treated with 1.3mg/kg of Aescin every 12 hours while the second group was left without treatment. Blood samples were collected in all animals at 0 (zero), 4am, 8am, 12am 4pm, 8pm and 12pm hours of the day to determine leucocyte and platelet count and to evaluate fibrinogen. Aescin produced: eosinopenia at 4am, 8am, 12am and 4pm hours; neutrophilia at 8am, 12am, 8pm, and 12pm hours; monocytopenia at 12pm hour and lymphopenia at 8am, 4am, 8pm an 12pm hours. There was an increase in fibrinogen level at 8pm and 12pm and a decrease in the platelet number at 0 (zero), 8am, 12am, 4pm and 8pm hours. Aescin determines unstable circadian variations in the number of leucocytes, platelets and in the amount of fibrinogen in health dogs.

DETERMINAÇÃO DOS VALORES MÉDIOS DE uréia NO SORO, LÍQUIDO CEFALORAQUIANO E HUMOR AQUOSO EM CÃES (canis familiaris)

SUMMARY The blood, cerebrospinal fluid and aqueous humor was collected in seventeen dogs with different diseases, for urea determination, that was realized with kit Labtest and posterior statistic evaluation. The urea determination can be made in the refered fluids with similar results.

UTILIZAÇÃO DE PAPEL DE FILTRO COMO ALTERNATIVA PARA AVALIAÇÃO DO TESTE DE SCHIRMER EM CÃES

In order to evaluated the Schirmer tear test 120 dogs (51 males and 69 females) of different breeds were observed. Four tipes of filter paper were used and prepared in strips of 4cm x 0,5cm. The papers were moisted into the conjuntival sac for one minute, then removed and the umidified segment were measured with a milimetrical rule. The results were analysed by Tukey test. The Melitta filter paper was more effective, and the determined parameters were: 1,4 +/- 1,5mm - KCS; 6,7 +/- 1,0mm - tear hyposecretion; 18,0 +/- 3,4mm -normal secretion and 29,9 +/- 6,7mm - epiphora.