Cláudio Roberto Scabelo Mattoso

Prevalence of Von Willebrand Disease in Dogs from São Paulo State, Brazil

The aims of the current study were to assess the prevalence of von Willebrand disease (vWD) in dogs from the region of Botucatu, São Paulo State, Brazil, and to evaluate laboratory tests to diagnose this disease. The study included 350 dogs of various ages, different breeds, and both sexes. Dogs included in the study had no historical or clinical evidence of abnormal bleeding. von Willebrand factor antigen (vWF: Ag), buccal mucosal bleeding time, activated partial thromboplastin time, and factor VIII activity were evaluated in their ability to diagnose vWD. The prevalence of vWD in dogs was 1.43% in the Botucatu region of Brazil. Determination of vWF: Ag was the best laboratory test to diagnose vWD.

Hematologic, serum biochemistry and urinary values for captive Crab-eating Fox (Cerdocyon thous) in São Paulo state, Brazil

The importance of studies with hematological, serum biochemistry and urinary values of Crab-eating Fox (Cerdocyon thous) is based on the need for health care and maintenance of those populations. This paper has the objective to investigate hematological, serum biochemistry and urinary physiological parameters of the Crab-eating fox, comparing gender and age differences. Blood samples were collected in 2003 from 52 animals of different Zoos in Sao Paulo state, Brazil; 7mL of blood was used to obtain a complete blood cell count (CBC) and the profile of the serum biochemistry. Moreover, 5mL of urine were collected for analysis. There was no difference in values for male and female animals, as for the CBC and serum biochemistry. Some hematological and serum biochemical parameters were influenced by age, showing significant differences. Urinalysis results were just demonstrated in a descriptive form. The studied values were, RBC 4.35±0.73 x 106 /µL, WBC 7.72±3.66 x 103 /µL (predominance of segmented neutrophils), platelets 227.06±111.58 x 103 /µL, urea 43.06±14.28mg/dL and creatinine 1.03±0.24mg/dL. Hematological, serum biochemistry and urinary values obtained in this study can be used as physiological values of the captive Crab-eating Fox. It is possible to conclude that wild species need their own reference values, differentiating animals in captivity from free-ranging animals.

Perfil eletroforético e concentração de imunoglobulinas G (IgG) do soro sanguíneo de cabras Saanen com mastite experimental induzida por Staphylococcus aureus suplementadas com vitamina E

The objective was to evaluate the electrophoretic profile of proteins and serum concentration of immunoglobulin G (IgG) in Saanen goats with mastitis experimentally induced by Staphylococcus aureus (dl-α-tocopherol acetated). 14 adult goats, (supplemented with vitamin E DL-α-tocopherol) primiparous pregnant, seronegative for caprine arthritis encephalitis (CAEV), clinically healthy, were divided into two groups of seven animals: Not supplemented group (G1) and group supplemented with 2.000 UI of DL-α-tocopherol (G2 Vit E), by intramuscular injection on the day of the parturition and seven days later. At the 9th day after delivery 300 UFCs of the S. aureus ATCC 225923 strain were inoculated into the left half of the mammary gland of each animal. The mastitis was determined through collection of milk samples for evidence of infection by means of bacteriological examination, somatic cell count (SCC) and California Mastitis Test (CMT). Then samples were collected after 12, 24, 48 and 72 hours, antimicrobial intra-mammary gland treatment was initiated, with new evaluation 48 hours after treatment. The electrophoretic profile of serum protein of the goats, showed five fractions, as follows: albumin and globulin (α, β1, β2 e γ-globulin). There was an increase in the production of γ-globulin and lower production of β2-globulin fraction 12 hours after infection, and faster decrease in the supplemented group, showing the influence of vitamin E in the production of acute phase proteins. There was no influence of vitamin E in the serum concentration of immunoglobulin G (IgG) in supplemented animals. The supplementation with vitamin E increased the concentration of immunoglobulin and decreased the production of acute phase proteins, probably the antioxidant effect minimizing the tissue injury during the inflammatory process in the mammary gland.

HEMATOLOGIC EVALUATION IN GUANS (PENELOPE OBSCURA) UNDER PHYSICAL RESTRAINT AND ISOFLURANE ANESTHESIA

Abstract Hematology is an important health indicator in birds, but the stress from capture or anesthesia can cause alterations in blood count. This study aimed to evaluate the differences found in blood counts among guans (Penelope obscura) under physical restraint and anesthetized with isoflurane. Thirty-two free-range guans from the Brazilian Institute of Environment and Renewable Natural Resources (IBAMA) Park were enrolled in the study. After physical restraint, blood samples were obtained from the ulnar vein and placed in heparinized tubes. The birds were anesthetized with isoflurane and after 45 min, when the animals were still anesthetized, a blood sample from the jugular vein was collected. Data were analyzed by paired t-test, and the differences were analyzed with Tukeys test. Decreases in red blood cell counts, hemoglobin concentrations, and packed cell volumes, and increased mean corpuscular hemoglobin concentrations were observed when animals were under general anesthesia. Decreases in total plasma protein, fibrinogen, and thrombocytes were detected after anesthesia. It was concluded that isoflurane influences the hematologic parameters, and those effects should be considered when interpreting hematologic values.