Cristina Viana-Niero
Federal University of São Paulo
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Publication
Featured researches published by Cristina Viana-Niero.
Journal of Clinical Microbiology | 2008
Cristina Viana-Niero; Karla Valéria Batista Lima; Maria Luiza Lopes; Michelle Christiane da Silva Rabello; Lourival Rodrigues Marsola; Vânia Cristina Ribeiro Brilhante; Alan Mitchel Durham; Sylvia Cardoso Leão
ABSTRACT An outbreak of infections affecting 311 patients who had undergone different invasive procedures occurred in 2004 and 2005 in the city of Belém, in the northern region of Brazil. Sixty-seven isolates were studied; 58 were from patients who had undergone laparoscopic surgeries, 1 was from a patient with a postinjection abscess, and 8 were from patients who had undergone mesotherapy. All isolates were rapidly growing nonpigmented mycobacteria and presented a pattern by PCR-restriction enzyme analysis of the hsp65 gene with BstEII of bands of 235 and 210 bp and with HaeIII of bands of 200, 70, 60, and 50 bp, which is common to Mycobacterium abscessus type 2, Mycobacterium bolletii, and Mycobacterium massiliense. hsp65 and rpoB gene sequencing of a subset of 20 isolates was used to discriminate between these three species. hsp65 and rpoB sequences chosen at random from 11 of the 58 isolates from surgical patients and the postinjection abscess isolate presented the highest degrees of similarity with the corresponding sequences of M. massiliense. In the same way, the eight mesotherapy isolates were identified as M. bolletii. Molecular typing by pulsed-field gel electrophoresis (PFGE) grouped all 58 surgical isolates, while the mesotherapy isolates presented three different PFGE patterns and the postinjection abscess isolate showed a unique PFGE pattern. In conclusion, molecular techniques for identification and typing were essential for the discrimination of two concomitant outbreaks and one case, the postinjection abscess, not related to either outbreak, all of which were originally attributed to a single strain of M. abscessus.
Journal of Clinical Microbiology | 2009
Sylvia Cardoso Leão; Enrico Tortoli; Cristina Viana-Niero; Suely Yoko Mizuka Ueki; Karla Valéria Batista Lima; Maria Luiza Lopes; Jesús Yubero; Maria Carmen Menendez; Maria Jesus Garcia
ABSTRACT An outbreak of postsurgical infections caused by rapidly growing mycobacteria has been ongoing in Brazil since 2004. The degrees of similarity of the rpoB and hsp65 sequences from the clinical isolates and the corresponding sequences from both the Mycobacterium massiliense and the M. bolletii type strains were above the accepted limit for interspecies variability, leading to conflicting identification results. Therefore, an extensive characterization of members of the M. chelonae-M. abscessus group was carried out. The M. abscessus, M. chelonae, M. immunogenum, M. massiliense, and M. bolletii type strains and a subset of clinical isolates were analyzed by biochemical tests, high-performance liquid chromatography, drug susceptibility testing, PCR-restriction enzyme analysis of hsp65 (PRA-hsp65), rpoB, and hsp65 gene sequencing and analysis of phylogenetic trees, DNA-DNA hybridization (DDH), and restriction fragment length polymorphism (RFLP) analysis of the 16S rRNA gene (RFLP-16S rRNA). The clinical isolates and the M. abscessus, M. massiliense, and M. bolletii type strains could not be separated by phenotypic tests and were grouped in the phylogenetic trees obtained. The results of DDH also confirmed the >70% relatedness of the clinical isolates and the M. abscessus, M. massiliense, and M. bolletii type strains; and indistinguishable RFLP-16S rRNA patterns were obtained. On the contrary, the separation of clinical isolates and the M. abscessus, M. massiliense, and M. bolletii type strains from M. chelonae and M. immunogenum was supported by the results of PRA-hsp65, DDH, and RFLP-16S rRNA and by the rpoB and hsp65 phylogenetic trees. Taken together, these results led to the proposition that M. abscessus, M. massiliense, and M. bolletii represent a single species, that of M. abscessus. Two subspecies are also proposed, M. abscessus subsp. abscessus and M. abscessus subsp. massiliense, and these two subspecies can be distinguished by two different PRA-hsp65 patterns, which differ by a single HaeIII band, and by differences in their rpoB (3.4%) and hsp65 (1.3%) sequences.
PLOS ONE | 2013
Layla Farage Martins; Luciana Principal Antunes; Renata C. Pascon; Júlio Cezar de Oliveira; Luciano Antonio Digiampietri; Deibs Barbosa; Bruno Malveira Peixoto; Marcelo A. Vallim; Cristina Viana-Niero; Éric Hainer Ostroski; Guilherme P. Telles; Zanoni Dias; João Batista da Cruz; Luiz Juliano; Sergio Verjovski-Almeida; Aline M. da Silva; João C. Setubal
Composting operations are a rich source for prospection of biomass degradation enzymes. We have analyzed the microbiomes of two composting samples collected in a facility inside the São Paulo Zoo Park, in Brazil. All organic waste produced in the park is processed in this facility, at a rate of four tons/day. Total DNA was extracted and sequenced with Roche/454 technology, generating about 3 million reads per sample. To our knowledge this work is the first report of a composting whole-microbial community using high-throughput sequencing and analysis. The phylogenetic profiles of the two microbiomes analyzed are quite different, with a clear dominance of members of the Lactobacillus genus in one of them. We found a general agreement of the distribution of functional categories in the Zoo compost metagenomes compared with seven selected public metagenomes of biomass deconstruction environments, indicating the potential for different bacterial communities to provide alternative mechanisms for the same functional purposes. Our results indicate that biomass degradation in this composting process, including deconstruction of recalcitrant lignocellulose, is fully performed by bacterial enzymes, most likely by members of the Clostridiales and Actinomycetales orders.
Microbes and Infection | 2008
Alessandra Marques Cardoso; Eduardo Martins de Sousa; Cristina Viana-Niero; Fernando Bonfim de Bortoli; Zilah Cândida das Neves; Sylvia Cardoso Leão; Ana Paula Junqueira-Kipnis; André Kipnis
A cluster of surgical site infection cases after arthroscopic and laparoscopic procedures occurred between 2005 and 2007 in Goiânia, in the central region of Brazil. Nontuberculous mycobacteria (NTM) were isolated from samples (exudates from cutaneous abscesses) from 18 patients of seven private hospitals. There were no reports of post-surgical arthroscopic and laparoscopic mycobacterial infections in Goiânia apart from this period. The 18 isolates were identified as Mycobacterium massiliense by PCR-restriction digestion of the hsp65 gene, pulsed-field gel electrophoresis (PFGE) comparisons, and rpoB partial gene sequencing. All isolates were typed as a single clone, indicating that they have the same origin, which suggests a common source of infection for all patients.
Journal of Clinical Microbiology | 2001
Cristina Viana-Niero; Cristina Gutierrez; Christophe Sola; Ingrid Filliol; Fadila Boulahbal; Véronique Vincent; Nalin Rastogi
ABSTRACT A collection of 105 clinical isolates originally identified asMycobacterium africanum were characterized using both phenotypic and genotyping methods. The phenotypic methods included routine determination of cultural properties and biochemical tests used to discriminate among the members of the M. tuberculosiscomplex, whereas genotypic characterization was based on IS6110-restriction fragment length polymorphism (IS6110-RFLP) analysis, IS1081-RFLP analysis, direct repeat-based spacer oligonucleotide typing (spoligotyping), variable number of tandem DNA repeats (VNTR), and the polymorphism of the oxyR, pncA, and mtp40 loci. The results obtained showed that a majority of M. africanumisolates were characterized by a specific spoligotyping pattern that was intermediate between those of M. tuberculosis andM. bovis, which do not hybridize with spacers 33 to 36 and spacers 39 to 43, respectively. A tentative M. africanum-specific spoligotyping signature appeared to be absence of spacers 8, 9, and 39. Based on spoligotyping, as well as the polymorphism of oxyR and pncA, a total of 24 isolates were excluded from the final study (19 were identified asM. tuberculosis, 2 were identified as M. canetti, and 3 were identified as M. bovis). The remaining 81 M. africanum isolates were efficiently subtyped in three distinct subtypes (A1 to A3) by IS6110-RFLP analysis and spoligotyping. The A1 and A2 subgroups were relatively more homogeneous upon spoligotyping than A3. Further analysis of the three subtypes by VNTR corroborated the highly homogeneous nature of the A2 subtype but showed significant variations for subtypes A1 and A3. A phylogenetic tree based on a selection of isolates representing the three subtypes using VNTR and spoligotyping alone or in combination confirmed the subtypes described as well as the heterogeneity of subtype A3.
Future Microbiology | 2010
Sylvia Cardoso Leão; Cristina Viana-Niero; Cristianne Kayoko Matsumoto; Karla Valéria Batista Lima; Maria Luiza Lopes; Moises Palaci; David Jamil Hadad; Solange Alves Vinhas; Rafael Silva Duarte; Maria Cristina S. Lourenço; André Kipnis; Zilah Cândida das Neves; Betina Mendez Alcântara Gabardo; Marta Osório Ribeiro; Ludmila Baethgen; Denise Brandão de Assis; Geraldine Madalosso; Erica Chimara; Margareth Pretti Dalcolmo
AIM Our aim is to investigate if the clusters of postsurgical mycobacterial infections, reported between 2004 and 2008 in seven geographically distant states in Brazil, were caused by a single mycobacterial strain. MATERIALS & METHODS Available information from 929 surgical patients was obtained from local health authorities. A total of 152 isolates from surgical patients were identified by PCR restriction enzyme analysis of the hsp65 gene (PRA-hsp65) and sequencing of the rpoB gene. Isolates were typed by pulsed-field gel electrophoresis (PFGE) using two restriction enzymes, DraI and AseI. A total of 15 isolates not related to surgical cases were analyzed for comparison. RESULTS All isolates were identified as Mycobacterium abscessus ssp. massiliense. Isolates from surgical patients and one sputum isolate grouped in a single PFGE cluster, composed of two closely related patterns, with one band difference. A total of 14 other isolates unrelated to surgical cases showed distinctive PFGE patterns. CONCLUSION A particular strain of M. abscessus ssp. massiliense was associated with a prolonged epidemic of postsurgical infections in seven Brazilian states, suggesting that this strain may be distributed in Brazilian territory and better adapted to cause surgical-site infections.
Jornal Brasileiro De Pneumologia | 2004
Cristina Viana-Niero; Sylvia Cardoso Leão
Os bacilos que causam a tuberculose estaoagrupados no complexo Mycobacteriumtuberculosis, composto por: M. tuberculosis, M.bovis subsp. bovis, M. africanum e M. microti,alem da cepa vacinal M. bovis BCG. Tem sidoproposta a inclusao de novas especies nestecomplexo, como M. canettii, uma variante de M.tuberculosis encontrada na regiao da Somalia
Journal of Clinical Microbiology | 2009
Fabíola Karla Ribeiro; E. M. Lemos; David Jamil Hadad; Sylvia Cardoso Leão; Cristina Viana-Niero; Reynaldo Dietze; John L. Johnson; K. D. Eisenach; Moises Palaci
ABSTRACT Low-colony-number counts on solid media are considered characteristic of cross-contamination, although they are normally observed in true-positive cultures from some groups of patients. The aim of this study was to evaluate low-yield growth cultures as a microbiological marker for cross-contamination. We evaluated 106 cultures with <15 colonies from 94 patients, and the proportions of false-positive cultures were 0.9% per sample and 1.1% per patient, which indicates that low-yield growth is not a reliable marker of cross-contamination.
BMC Microbiology | 2016
James Daltro Lima-Junior; Cristina Viana-Niero; Daniel V. Conde Oliveira; Gabriel Esquitini Machado; Michelle Cristiane da Silva Rabello; Joaquim Martins-Junior; Layla Farage Martins; Luciano Antonio Digiampietri; Aline M. da Silva; João C. Setubal; Daniel A. Russell; Deborah Jacobs-Sera; Welkin H. Pope; Graham F. Hatfull; Sylvia Cardoso Leão
BackgroundA large collection of sequenced mycobacteriophages capable of infecting a single host strain of Mycobacterium smegmatis shows considerable genomic diversity with dozens of distinctive types (clusters) and extensive variation within those sharing evident nucleotide sequence similarity. Here we profiled the mycobacterial components of a large composting system at the São Paulo zoo.ResultsWe isolated and sequenced eight mycobacteriophages using Mycobacterium smegmatis mc2155 as a host. None of these eight phages infected any of mycobacterial strains isolated from the same materials. The phage isolates span considerable genomic diversity, including two phages (Barriga, Nhonho) related to Subcluster A1 phages, two Cluster B phages (Pops, Subcluster B1; Godines, Subcluster B2), three Subcluster F1 phages (Florinda, Girafales, and Quico), and Madruga, a relative of phage Patience with which it constitutes the new Cluster U. Interestingly, the two Subcluster A1 phages and the three Subcluster F1 phages have genomic relationships indicating relatively recent evolution within a geographically isolated niche in the composting system.ConclusionsWe predict that composting systems such as those used to obtain these mycobacteriophages will be a rich source for the isolation of additional phages that will expand our view of bacteriophage diversity and evolution.
Genome Announcements | 2013
Sylvia Cardoso Leão; Cristianne Kayoko Matsumoto; Cristina Viana-Niero; Rommel Thiago Jucá Ramos; Adriana Ribeiro Carneiro; Maria Silvanira Barbosa; Karla Valéria Batista Lima; Maria Luiza Lopes; Vasco Azevedo; Artur Silva
ABSTRACT An epidemic of surgical-site infections by a single strain of Mycobacterium abscessus subsp. bolletii affected >1,700 patients in Brazil from 2004 to 2008. The genome of the epidemic prototype strain M. abscessus subsp. bolletii INCQS 00594, deposited in the collection of the National Institute for Health Quality Control (INCQS), was sequenced.