Daniel Curvello de Mendonça Müller

Adaptação do índice de massa corporal humano para cães

The index of corporal mass (ICM) is broadlly used to measure the corporal mass of adults. A high fat index is related to heart and circulation problems, infertility, diabetes, arthritis, difficulty to move around or breath and problems with cicatrization. Even though there are several studies about these problems, there is no specific method to evaluate weight excess in dogs objectively and precisely. The purpose of this research was to determine an index of corporal mass for dogs based on the existing IMC for human beings. Two hundred and forty six dogs without defined-breed and with different body conditions were analysed. They were not only measured and weighed, but also evaluated by two distinct veterinarians based on real subjective patterns, which can determine the corporal score. These procedures enabled the acknowledgement of the attained index. The conclusion was that IMCC between 11,8 and 15kg m-2 represent the ideal pattern for dogs of average size that weigh between 10 and 25kilos.

Técnicas e sítios de coleta de medula óssea em cães e gatos

Currently, the research focuses on bone marrow as a potential source for the treatment of diseases, since its use as a diagnosis is already largely employed. This paper aims to promote a practical review on the best access to the bone marrow and the importance of this material as diagnosis and treatment. Sampling techniques are observed and detailed by the authors, in order to facilitate the procedures and make them safer.

Colheita de medula óssea em cães: modelo para obtenção da fração total de células mononucleares

ABSTRACT In the present research a new protocol to harvest10ml kg -1 femoral bone marrow (BM) was developed to allowisolation, quantification and to test the mononuclear cell (MC)fraction viability. Forty male or female stray dogs, aging andweighting around two years old and 10kg respectively, weresubmitted to aseptic bone marrow harvest in a surgicalenvironment. To achieve an ideal cell count of MC, ananatomical Steis needle was used during the procedure, whichfavored the indicated volume harvest in a shorter period oftime without interfering cellular viability. A bone marrowcollection kit was also used to filter bone fragments whilemaintaining harvested MC integrity during blood packaging.Meanwhile BM harvesting was conducted, animals peripheralblood collection was performed (pre, trans and post-operatory)to hematological evaluations and autologous blood transfusionwas made to overcome the increased hemoglobin fall thattakes place in the initial harvesting moments. The harvestedand filtered BM was slowly placed over a Histopaque densitygradient (1.077g ml

Implante sintético como estabilizador articular, após desmotomia dos ligamentos cruzados de cães: proposição de técnica

The ligament rupture (LC) is an illness diagnosed in most dogs with joint problems, and the most common cause of degenerative joint disease of the knee joint. This study aimed to evaluate clinically the action of a polypropylene mesh as the only substitute for cranial cruciate ligament and flow of dogs, highlighting features of the technique proposed here. It was used 20 dogs which underwent unilateral desmotomy cranial and caudal LC. Twenty-one days after the desmotomy, all were treated surgically by the technique of open reduction, with implantation of polypropylene replacing only the cranial LC. The proposed technique has shown rapid implementation and low soft tissue dissection, early return to ambulation, adequate strength of the implant without associated external immobilization and absence of articular reaction until 90 days after surgery.

Modificações do fixador externo para osteossíntese umeral em pombos domésticos

This paper was aimed at evaluating two changes in the external fixators for osteosynthesis of the humeral fracture that induce the correction of the humeral fracture in pigeons. The reduction of diaphyseal fracture was made with an intramedulary pin associated to another perpendicular one inserted into the distal segment (Group A - GA); in the Group B (GB) an additional pin was used in the proximal fragment. All the pigeons were evaluated clinically and radiographically up to 60 days, except for four of each group which were submitted to euthanasia at 15, 22, 29 and 36 days for the macro and microscope exams. The flight test was successful in all pigeons. The bone calus was radiografically visible only in the GA; the mean time and the standard deviation for flight was 32.25 ± 6.5 days for the GA and 39.8 ± 3.83 for the GB. The mean time and standard deviation for fracture healing was 36 ± 0 days in the GA and 34 ± 3.4 in the GB. The two types of external fixator evaluated were efficient to stabilized the fracture site allowing complete consolidation and return to function. The use of the external fixator with two perpendicular pins provides more stability at the fracture site although it is necessary more time for the surgery procedure.

Biomarcadores inflamatórios e indicadores de estresse oxidativo em cadelas submetidas à ovariossalpingohisterectomia convencional, por NOTES híbrida e NOTES total

This study aimed at comparing the inflammatory response and the oxydative profile of the conventional ovarysalpingohysterectomy (OSH) technique to Totally Natural Orifice Transluminal Endoscopic Surgery (NOTES) and Hybrid NOTES. Group of fifteen female dogs was used for each technique. Blood samples were taken before the surgical procedure (basal) and 3, 6, 12, 24, 48 and 72h postoperative. The catalase activity was increased in the three studied groups. Nevertheless, lipid peroxidation, measured by TBARS (thiobarbituric acid reactive substances) levels, was higher in the conventional group and almost indistinguishable in the total NOTES group. In the three analyzed groups, both butyrylcholinesterase and acetylcholinesterase activities were increased as well as the neutrophil counts during the first post-surgical hours. It is possible to conclude that systemic inflammation occurs in a similar way in the three operative techniques; however, total NOTES technique presents lower levels of cellular oxidative damage, particularly if compared to the conventional approach.

Ovariossalpingo-histerectomia em cadelas: comparação da dor e análises cardiorrespiratória, pressórica e hemogasométrica nas abordagens convencional, por NOTES híbrida e NOTES total

A cirurgia endoscopica por orificios naturais (NOTES) e um novo conceito de abordagem videocirurgica, que surge como alternativa a cirurgia convencional, eliminando incisoes abdominais e as possiveis complicacoes relacionadas a ela. A proposta deste artigo foi comparar tecnicas de ovariossalpingo-histerectomia (OSH) em cadelas, buscando determinar o procedimento que oferece menores alteracoes hemodinâmicas e menos estimulos dolorosos trans e pos-operatorio. Para tanto, foram utilizadas 21 cadelas alocadas em tres grupos. No primeiro, os pacientes foram submetidos a OSH por celiotomia (GC), no segundo por meio da tecnica de NOTES hibrida (GNH) e no terceiro (GNT), utilizando-se a tecnica de NOTES total. O tempo cirurgico do GNH foi significativamente maior que nos demais grupos. Em ambos os grupos de cirurgia NOTES, verificou-se diminuicao das pressoes arteriais medias e diastolicas no transoperatorio. Apenas o grupo GNH desenvolveu acidose severa no transoperatorio. Levando-se em consideracao as avaliacoes da dor, apenas o grupo convencional necessitou de analgesia resgate transcirurgica. No pos-operatorio, observou-se que os caes do GC apresentaram indices mais elevados na escala visual analogica e na escala de Melbourne que os animais dos demais grupos, sendo necessaria analgesia resgate em 100% deles. Em contraste, no GNT nenhum dos caes requereram complementacao analgesica pos-operatoria. Conclui-se que a tecnica de OSH por NOTES total apresenta parâmetros cardiorrespiratorios e hemogasometricos semelhantes a tecnica convencional e mais estaveis que a tecnica de NOTES hibrida, bem como resulta em menor dor trans e pos-operatoria que a tecnica convencional.

Biópsia pulmonar incisional por toracoscopia paraxifoide transdiafragmática com dois portais em cães

There are many respiratory diseases in small animals, as well as the possibilities of diagnoses, as laboratory and radiographic exams, bronchoalveolar lavage and biopsies. This research aimed at assessing the results of transdiaphragmatic paraxiphoid thoracoscopies in pulmonary biopsies of thirteen clinically healthy dogs. Under general anesthesia, each dog was insufflated with 30ml kg-1 of air in each hemithorax; two trocars were placed between the xiphoid appendix and the costal arch through the diaphragm. An endoscope was introduced by the first portal; while the second portal was used to allow access to the nippers in the biopsy forceps. Then, these nippers were removed and replaced by chest tube drainage. During the procedures, respiratory frequency, heart rate, oxygen saturation, arterial pressure and central venous pressure (CVP), hemogasometric parameters were checked. Within 48 hours after the surgery, drains were or drain was removed, with minimal production of air and/or liquid. In conclusion, this is a fast and safe technique, which is free of trans and post surgical complications, thus allowing the collection of sufficient material for pulmonary histological evaluation.

Artrografia com meio de contraste positivo e duplo contraste na articulação do úmero de cães

The double contrast artrography is a diagnostic method in which positive contrast material (MCP) is injected in the joint space followed by negative contrast in order to outline lesions on the articular surfaces and adjacent structures. This technique is an effective and minimally invasive procedure already consolidated in human medicine as well as in the equine species with better results than the ones achieved by using usual diagnostic methods. In dogs this is not widely applied. The present study aimed to describe and evaluate the double contrast artrography technique applied to the humeral joint of canine species to compare with artrography with MCP in the evaluation of the articular surface of humerus head, intra-articular portion of the biceps muscle tendon, and articular capsule of eleven adult dogs. The double contrast within the joint has been produced by combining a small amount of iodine-based half of positive contrast covering the articular surfaces and capsular limits and a larger amount of a negative contrast filling the intra-articular space. The information collected from four radiologists have allowed to conclude that in the evaluation of the articular surface of the humerus head the double contrast artrography technique determinates a better image than with MCP. In addition, in the evaluation of the outline of the intra-articular portion of the biceps muscle tendon the artrography with MCP produced a better image than with double contrast artrography.

Análise biomecânica ex vivo de dois métodos de osteossíntese de ílio em cães

This study evaluated the biomechanical use of cortical allografts preserved in honey for the stabilization of transverse osteotomy of ilium in dogs, as well as the use of wire hemicerclage alone against the bending forces. Thirteen canine were tested bilaterally, where one side was stabilized with bone graft and the other with wire hemicerclage alone. The prototypes stabilized with bone graft were statistically more resistant to strength flexion than those wire hemicerclage used alone. The results showed that the use of cortical bone implants is a viable alternative for fixing the iliac osteotomy in dogs.