Dedi Soedharma

New cytotoxic cembranoid from indonesian soft coral Sarcophyton sp.

Context: Sarcophyton is a soft coral species that contains various secondary metabolites with cytotoxic activity. The production of cytotoxic compounds in soft corals is suggested as their allelochemical to win space competition. Therefore, if a particular soft coral species dominates a reef area, it may suggest to contain interesting bioactive compounds. Aims: This research aimed to characterize the cytotoxic compounds in dominant soft coral species (Sarcophyton sp.) on the reef at the Western side of Mahengetang Island, Indonesia. Subjects and Methods: Isolation of cytotoxic compounds through ethanol extracts had been done with preparative high-performance liquid chromatography and bioassay-guided fractionation by MCF-7 (breast) cancer cell lines. The structures of each cytotoxic compounds were elucidated on the basis of mass and nuclear magnetic resonance spectroscopic studies. Results: Elucidation through all compounds found a new cembranoid, namely, 2-hydroxy-crassocolide E (1), alongside with 5 known cembranoids; sarcophytoxide (2), sarcrassin E (3), 3,7,11-cembreriene-2,15-diol (4), 11,12-epoxy-Sarcophytol A (5), and sarcophytol A (6). All of these cembranoids were showed to inhibit the growth of MCF-7 (breast) cancer, with 50% inhibition of tumor cell lines growth lower than 30 mg/L. Conclusions: Results of this study suggest that a soft coral species which dominate a reef area is a potential source for various bioactive compounds.

Management of Small Pelagic Fishery Based on Environment Carrying Capacity

One of the potency for development of Sangihe Islands is sea and the availability of fish resources which is relatively high, both in terms of quantity and diversity. One of the fish types with high quantity in this area are pelagic fish group. This research aims to analyze the management of small pelagic fishing in order to maintain its sustainability. The research result indicate that the average sustainable production of the small pelagic for 20 years (1988-2007) observation was 3,128.45 tons/year. Depreciation value has reduced the rents value received by fishermen. With the method of the present value, the revenues from the market at discount rate of 15% should be Rp 274.97 billion. However, due to depreciation, the amount received just Rp 118.59 billion only. For the optimal and sustainable fisheries management, the effort level for small pelagic capture should be 5,342 trips/year at discount rate of 15%. To maintain the sustainability, the level should be lowered down around 46% from the present level in small pelagic management.

The Oogenesis of Sclerectinian Corals Caulastrea furcata and Lobophyllia corymbosa

T-2 toxin is a toxic and teratogenic mycotoxin produced by Fusarium tricintum which may contaminate cereal, seed, and food. The aim of this research is to find out the effects of T-2 Toxin on preimplantion embryos and fetuses of Swiss Webster mice. Pregnant female of Swiss Webster mice on 0 or 2 day of gestation was injected intraperitoneally with T-2 toxin at doses 0.05 or 0.10 mg/kg body weight (bw) and the dam was observed at 3.5 and 18 days of gestation. At 0 day of gestation, embryos were arrested at one to eight cell and uncompacted morulae stages (P Key words: Fusarium tricintum, T-2 toxin, mycotoxin, preimplantation embryos, embryotoxic