Denny Satria

Antioxidant and Antiproliferative Activities of an Ethylacetate Fraction of Picria Fel-Terrae Lour. Herbs

Background: Excessive production of oxygen free radicals and imbalance in the mechanisms responsible for antioxidant protection may result in the onset of many diseases including breast cancer. Objective: To evaluate antiproliferative and antioxidant activity of an ethylacetate fraction (EAF) of Picria fel-terrae Lour. Herbs in the T47D cell line. Methods: Phenolic and total flavonoid contents in EAF were determined. EAF was tested for cytotoxicity and effects on the cell cycle and apoptosis, as well as antioxidant activity. Results: EAF was found to contain high levels of phenolic agents (92.88 ± 0.50 mg GAE/g), total flavonoid (84.39 ± 0.07 mg QE/g). The EAF of Picria fel-terrae Lour. herbs was found to have an IC50 of 62.98µg/mL, caused accumulation in G0-G1 and S phase and increased early and late apoptosis. Antioxidant activity in DPPH assays gave an IC50 of 166.90 ± 0.10 µg/mL. Conclusions: The results reveal that EAF of Picria fel-terrae Lour. herbs has antiproliferative activity and strong antioxidant potential. Further studies are now needed to isolate the responsible antiproliferative and antioxidant components.

Antimigration Activity of an Ethylacetate Fraction of Zanthoxylum acanthopodium DC. Fruits in 4T1 Breast Cancer Cells

Objective: This study was carried out to investigate the antimigration activity of Zanthoxylum acanthopodium DC. in the 4T1 breast cancer cell line. Methods: Zanthoxylum acanthopodium DC. fruit powder was extracted by maceration method with n-hexane and ethylacetate solvents. Cytotoxicity and proliferation were assessed using the MTT method and the cell cycle by flow cytometry. In addition, wound healing assays were conducted by a microscopic method, and expression of COX-2 and VEGFR-2 were determined using qRT-PCR. Results: The IC50 of the ethylacetate fraction (EAF) was 48.1 ± 1.06 µg/mL. The EAE at a concentration 10 µg/mL with viable cells was 62.3 ± 0.28% after 72 h incubation, with accumulation in the G2-M phase, inhibition of cell migration in the wound healing assay, and decrease in expression of COX-2 (0.62 ± 0.01) and VEGFR-2 (0.39 ± 0.003). Conclusion: The results reveal that an ethylacetate fraction of Zanthoxylum acanthopodium DC. fruits provides effective antimigration effects. Further studies are now planned to assess the potential of the ethylacetate fraction to inhibit angiogenesis in breast cancer and determine underlying mechanisms.

TOTAL PHENOLIC AND FLAVONOID CONTENTS AND ANTIOXIDANT ACTIVITY OF ETHANOL FRACTION OF PICRIA FEL-TERRAE (LOUR.) HERBS

Objective: To evaluate total phenolic, flavonoid content (TFC and TPC) andantioxidant activity of ethyl acetate fraction (ETF) of Picria fel-terrae Lour. herbs. Methods: TPC and TFC in ETF were determined by Folin–Ciocalteu and colorimetric methods, and antioxidant activity was determined by 1,1-diphenyl- 2-picrylhydrazyl (DPPH) method. Results: ETF was found to contain low levels of phenolic (17.71±1.26 mg gallic acid equivalent/g), total flavonoid (14.43±0.03 mg quercetin equivalents/g). Antioxidant activity from DPPH assay was measured as inhibitory concentration 25.72±0.13 μg/mL. Conclusions : The results reveal that ETF of P. fel-terrae Lour. herbs has strong antioxidant activity. Our further study is to the isolation of compounds which responsible for antioxidant components.

ANTICANCER ACTIVITY OF Β-SITOSTEROL FROM PLECTRANTHUS AMBOINICUS (LOUR. SPRENG.) LEAVES: IN VITRO AND IN SILICO STUDIES

Objective: I²-sitosterol is the steroid compound which is an important nutrient in the diet meal, hydrophobic and soluble in organic solvents and considered as a good biomarker due to its biological activity.Methods: In vitro study was using 2,5-diphenyl tetrazolium bromide method towards T47D, MCF-7, HeLa, and WiDr cell lines. In silico docking using PLANTS program and visualized by Yasara program. The model of three dimension enzyme structures used in this research were epidermal growth factor receptor (EGFR), phosphatidylinositol-3-kinase (PI3K), estrogen receptor-alpha (ER-I±), ER-beta (ER-I²), and human EGFR 2 (HER-2). Two and three dimensions of I²-sitosetrol, ZSTK474, and tamoxifen as the standard were generated using Marvin Sketch program.Results: I²-sitosterol was found to have inhibitory concentration 50% of 0.55; 0.87; 0.76, and 0.99 mM. I²-sitosterol and ZSTK474 were inhibited EGFR and PI3K with docking score −92.8195; −91.7920 and −91.7470; −94.7491 I²-sitosterol and tamoxifen were inhibited ER-I±, ER-I² and HER-2 with docking score −78.5570; −89.535, −68.7717; −52.008 and −90.4908; −50.5576, respectively.Conclusion: Based on the results above that shows I²-sitosterol provide effective as anticancer.Â