Diana Rachmawati

Effect of The Phytase Enzyme Addition in The Artificial Feed on Digestibility of Feed, Feed Conversion Ratio and Growth of Gift Tilapia Saline Fish (Oreochromis niloticus) Nursery Stadia I

The purpose of this study was to determine the effect of adding the phytase enzyme in the artificial feed on digestibility of feed, feed conversion ratio and growth of gift tilapia saline fish (Oreochromis niloticus) nursery stadia I. The fish samples in this study used gift tilapia saline fish (O. niloticus) with an average weight of 0,62 ± 0,008 g/fish and the stocking density of 1 fish1 L. Experimental method used in this study was completely randomized design with 4 treatments and 3 repetitions. The treatments were by adding phytase enzyme in artificial feed with the different level of doses those were A (0 FTU kg1 feed), B (500 FTU kg1 feed), C (1000 FTU kg1 feed) and D (1500 FTU kg1 feed). The results show that the addition of phytase enzyme was significantly (P 0.05) affected on Survival Rate (SR) of gift tilapia saline fish. The optimum doses of phytase enzyme on RGR, FCR, PER, ADCP and ADCF of gift tilapia saline fish ranged from 1060 to 1100 FTU kg-1 feed.

Engineering Technology Of Fish Farming Floating Nets Cages On Polka Dot Grouper (Cromileptes Altivelis) Used Artificial Feed Enriched Phytase Enzyme

One solution is to utilize engineering technology cultivation floating cage net polka dot grouper (ducker grouper), which is given artificial feed enriched with phytase enzymes. The objectives of this study was to examine the use of technology engineering floating net on ducker grouper on artificial feed that is enriched with different dose phytase enzymes to accelerate growth and survival. The research method used ducker grouper fish size 15,5 ± 0,5 cm in the net cages unit (1 m x 1 m x 1 m), 250 fish per cage, using 12 cages. Each net-cages was made of polyethylens netting, mesh size 12.5 mm. with complete randomized design (CRD) 4 treatment and 3 replication were feed Artificial enriched of phytase enzyme with the doses of A (0 FTU kg-1 diet), B (200 FTU kg-1 diet), C (500 FTU kg-1 diet), and D (800 FTU kg-1 diet) phytase enzyme. Feed was given 2 times a day in the morning and afternoon with 5% biomass per day. Data includes the growth of absolute weight polka dot grouper, FCR, and survival rate analyzed variety and Test Tukey.The result of the research showed that the difference of artificial feeding enriched phytase enzyme significantly (P <0,05) to growth, food conversion ratio (FCR), survival rete of polka dot grouper. The best treatment at C (500 mg / kg of feed) increase growth of absolute weight of 128.75 g, 1.75 (FCR), and a survival rate of 93.5%.

PENGARUH ENZIM PROTEASE PAPAIN DALAM PAKAN BUATAN TERHADAP PERTUMBUHAN DAN NET PROTEIN UTILIZATION BENIH LELE SANGKURIANG YANG DIBUDIDAYA DI DESA WONOSARI, KECAMATAN BONANG, KABUPATEN DEMAK

Availability of diet in Sangkuriang catfish (Clarias gariepinus) cultivation is becoming a main concern. One of the problems in the artificial diet is that the protein content could not fully be utilized by the catfish. To solve the problem is by adding papain protease in the artificial diet. This study was conducted to evaluate the effect of papain protease enzyme in artificial diet on diet utilization efficiency and growth performance of sangkuriang catfish (Clarias gariepinus) fingerlings in the Village of Tambaksari, Sub-district of Rowosari, District of Kendal, and Central Java. The fingerlings with initial body weight averaging 4.23±0,30 g were cultivated with the density of 1 fingerling per liter. The fingerlings were obtained from Center for Hacthery, Muntilan. The fingerlings were fed with artificial diet in four different doses of papain enzyme: A (0%), B (0.75%), C (1.5%), and D (2.25%). The experiment used Complete Random Design with 4 (four) treatments and each treatment replicated 3 (three) times. The results show that the addition of papain protease enzyme significantly (P<0.01) affected on the spesific growth rate (SGR) and net protein utilization (NPU). It could be concluded that the addition of papain protease enzyme could increase the specific growth rate (SGR) and net protein utilization (NPU) of Sangkuriang catfish (Clarias gariepinus) fingerlings. Optimum papain protease enzyme dose on the specific growth rate was 2.25% per kg diet. The water quality during study was still in the viable ranges for Sangkuriang catfish (Clarias gariepinus) fingerlings cultivation. Key word: papain protease enzyme, artificial diet, growth, sangkuriang catfish fingerlings

Addition of Phytase Artificial Feed to Increase Digesting, Specific Growth and Survival Rate of Nile Tilapia fingerlings (Oreochromis niloticus)

The aims of this study were to examine the effects of phytase enzym and to determine the optimum dose of phytase enzym in artificial feed on digesting, specific growth rate and survival rate of Nile Tilapia fingerlings (Oreochromis niloticus). Nile Tilapia fingerlings (O. niloticus) used in this study were 120 animals with the weight of 3±0.02 g/animal. The fingerlings were raised in 6 weeks with the density of 1 animal/liter. Methodology used in this study was laboratory treatments with complete random design. The study consisted of four treatments and three repetitions. The treatments were addition of phytase enzym in artificial feed with the different level of doses; those were A (0 mg/(kg of feed)), B (500 mg/(kg of feed)), C (1.000 mg/(kg of feed)), and D (1.500 mg/(kg of feed)). The artificial feed with 30% protein content was in the dried and crumb pellet form which was added phytase enzym in every treatment. Data collected were specific growth rate, feed conversion ratio, protein efficiency ratio, raw protein digesting rate, total protein digesting rate, survival rate and water quality. Varian annalysis was used in this study. If the results were significant (p<0.05) and highly significant (p<0.01), double Duncan area test was conducted to determine the mean of different treatments. In order to find the optimum effect polynomial orthogonal test was used. Descriptive analysis was used to explain raw and total protein digesting rate and water quality. The results show that the addition of phytase enzym in artificial feed significantly affected on the specific growth and feed utilization ( feed and protein efficiency ratios); however, it did not significantly affect on the survival rates. The optimum dose of phytase enzym in artificial feed on the specific growth and feed utilization was at the level of 1,000 mg/(kg of feed). This level resulted in the highest of raw and total protein digesting rates of Nile Tilapia fingerlings (O. niloticus), compared to B, D, and A treatments. The raw and total protein digesting rates were of 84.88% and 71.27%. The water quality during study was in viable range for the Nile Tilapia fingerlings (O. niloticus) cultivation. Keywords : Nile Tilapia (Oreochromis niloticus), Phytase Enzym, Digesting, Specific Growth, Survival Rate