Johannes Hutabarat

Lupin ingredients in shrimp (Penaeus monodon) diets: influence of lupin species and types of meals

The nutritive value of five isonitrogenous practical diets containing different legume meals, whole or dehulled lupin (Lupinus albus) seed meal (WAD and DAD, respectively), dehulled lupin (L. angustifolius) seed meal (DND), lupin (L. angustifolius) protein concentrate (LPCD), and defatted soybean meal (SBD), was evaluated in two laboratory experiments (growout and digestibility studies) using juvenile (4.07±0.05 g, mean±SD) shrimp, Penaeus monodon. In the 42-day growout study, shrimp fed SBD or DND had significantly higher (P<0.05) growth rates than those fed LPCD, DAD or WAD. Protein conversion efficiency (PCE) and apparent protein utilization (APU) values of DND were significantly higher than those of LPCD, DAD or WAD. SBD and DND had significantly higher feed intake values and better feed conversion ratios (FCRs) than WAD and DAD. LPCD had a similar FCR to other diets but lower feed intake than SBD. Shrimp fed WAD had significantly poorer performance in all the above parameters than shrimp fed other diets except for DAD and LPCD. The diets SBD, DND, LPCD, and DAD had significantly higher (P<0.05) apparent dry matter digestibilities (ADMD) (68.3–71.4%) than WAD (64.4%). All diets had a similar apparent protein digestibility (APD) (87.1–89.1%) with a small, but significant difference being observed for the diets with the highest (DND) and the lowest (WAD) protein digestibility. Pellet water stability was significantly different (P<0.05) among the diets. The most stable pellet was displayed by LPCD, while WAD had the poorest water stability. Dehulling or concentrating lupin seed protein did not improve the nutritive value of lupin diets for juvenile P. monodon. The lupin seed meal of L. angustifolius was better utilized by P. monodon than that of L. albus and the nutritive value of L. angustifolius diet was equal to that of soybean (control) diet. Results of a feeding preference test also indicated that L. angustifolius diet was more attractive for juvenile P. monodon than L. albus diet.

White Shrimp (Litopenaeus vannamei) Culture using Heterotrophic Aquaculture System on Nursery Phase

Heterotrophic aquaculture system is an environmental friendly shrimp culture that has a huge potency to improve yields of Litopenaeus vannamei. Biofloc grown in a heterotrophic aquaculture system that can be used as an alternative feed for shrimp due to its high nutrition. Biofloc contains bacterial protein and polyhydroxybutyrate that are able to enhance growth. Biofloc also contains bacteria that have peptidoglycan and lipopolysaccharide on their cell walls. The aim of the research was to study the effect of heterotrophic aquaculture system on culturing of Litopenaeus vannamei during nursery phase. The experiment was arranged in split plot design in three replicateses. The treatments consisted of two factors namely various densities and different aquaculture systems. The aquaculture systems were autotrophic and heterotrophic aquaculture system, while densities were 1,000, 1,500, and 2,000 PLm-3. The result showed that there was no significant interaction between densities and aquaculture system toward the growth rate, protein efficiency ratio and yield of Litopenaeus vannamei. The heterotrophic aquaculture system was able to increase the yield of Litopenaeus vannamei on nursery phase. However heterotrophic aquaculture system did not significantly affect growth rate and protein efficiency ratio of Litopenaeus vannamei. While, the density significantly affected survival rate and yield of Litopenaeus vannamei.

The Growth Performance and Nutrient Quality of Asian Swamp Eel Monopterus albus in Central Java Indonesia in a Freshwater Aquaculture System with Different Feeds

ABSTRACT The Asian swamp eel (Monopterus albus) is a high protein content fish with delicious taste that can survive for 6–8 months. The growth performance and nutrient quality of Asian swamp eels in a freshwater aquaculture system in Central Java, Indonesia was studied using a completely randomized design. Golden snails, snails, silkworms, and earthworms were the feeding treatments at 5% of total body weight. The test animals weighed 5.68 ± 0.35 g after a 60-day culture, and the density was 36 eels/m2. Providing different types of feed had significant effects (P < 0.05) on relative growth rate (RGR), feed intake, and survival rate (SR) but did not significantly affect the protein efficiency ratio (P > 0.05). The best final weight, RGR, and SR results were 11.80 g, 2.64%, and 80.95% during a 60-day culture with silkworms used as feed. The nutrient quality in a similar treatment found with the best proximate composition was 76.90% protein, 3.24% fat, 25.19% palmitic acid, 15.41% oleic acid, 15.9% glutamic acid, 8.2% isoleucine, and 8.9% lysine.

PENGARUH ENZIM PROTEASE PAPAIN DALAM PAKAN BUATAN TERHADAP PERTUMBUHAN DAN NET PROTEIN UTILIZATION BENIH LELE SANGKURIANG YANG DIBUDIDAYA DI DESA WONOSARI, KECAMATAN BONANG, KABUPATEN DEMAK

Availability of diet in Sangkuriang catfish (Clarias gariepinus) cultivation is becoming a main concern. One of the problems in the artificial diet is that the protein content could not fully be utilized by the catfish. To solve the problem is by adding papain protease in the artificial diet. This study was conducted to evaluate the effect of papain protease enzyme in artificial diet on diet utilization efficiency and growth performance of sangkuriang catfish (Clarias gariepinus) fingerlings in the Village of Tambaksari, Sub-district of Rowosari, District of Kendal, and Central Java. The fingerlings with initial body weight averaging 4.23±0,30 g were cultivated with the density of 1 fingerling per liter. The fingerlings were obtained from Center for Hacthery, Muntilan. The fingerlings were fed with artificial diet in four different doses of papain enzyme: A (0%), B (0.75%), C (1.5%), and D (2.25%). The experiment used Complete Random Design with 4 (four) treatments and each treatment replicated 3 (three) times. The results show that the addition of papain protease enzyme significantly (P<0.01) affected on the spesific growth rate (SGR) and net protein utilization (NPU). It could be concluded that the addition of papain protease enzyme could increase the specific growth rate (SGR) and net protein utilization (NPU) of Sangkuriang catfish (Clarias gariepinus) fingerlings. Optimum papain protease enzyme dose on the specific growth rate was 2.25% per kg diet. The water quality during study was still in the viable ranges for Sangkuriang catfish (Clarias gariepinus) fingerlings cultivation. Key word: papain protease enzyme, artificial diet, growth, sangkuriang catfish fingerlings

Nutritional value of spiny lobsters (Panulirus sp.) from Southern Coast of Java

Five species of spiny lobsters are known to live in southern coast of Java. These lobsters are very popular seafood which was believed to have high nutritional value. However, nutrition content of these species from the area has not been investigated. This research was conducted to study nutrition content in these crustaceans. Five spiny lobsters i.e. Panulirus homarus, P. versicolor, P. ornatus, P. penicullatus, and P. longipes, were collected randomly from different locations at the southern coast of Java. Morphometric measurements were conducted prior to proximate analysis of these lobsters. All species of spiny lobsters investigated have similar carapace length. However, P. homarus and P. versicolor have the highest muscle weight. Proximate analysis shows that P. homarus also has high protein (24.18%) and carbohydrate content (55.68%) and lowest lipid content (6.18%) compare with other species. These results suggest that this lobster has best nutritional value for consumption.