Didem Sohretoglu

Antioxidant secondary metabolites from Geranium lasiopus Boiss. & Heldr.

Chromatographic studies on the EtOAc soluble portion of the MeOH extract of Geranium lasiopus led to the isolation of eight flavonoids (kaempferol (1), quercetin (2), quercetin 3-O-β-glucopyranoside (3), quercetin 3-O-β-galactopyranoside (4), kaempferol 3-O-α-rhamnopyranosyl-(1 → 6)-β-glucopyranoside (5), quercetin 3-O-α-rhamnopyranosyl-(1 → 6)-β-glucopyranoside (6), kaempferol 3-O-α-rhamnopyranosyl-(1 → 2)-β-glucopyranoside (7) and quercetin 3-O-α-rhamnopyranosyl-(1 → 2)-β-glucopyranoside (8)), two simple phenolic compounds (gallic acid (9) and its methyl ester (10)) and a hydrolysable tannin (pusilagin (11)). The structures of the compounds were elucidated by 1- and 2-dimensional NMR techniques (1H, 13C, COSY, HMBC, HMQC) and ESI-TOF-MS spectrometry. Inhibitory effects on H2O2-induced lipid peroxidation in human red blood cells of the different extracts of G. lasiopus, as well as isolated compounds, were investigated. All tested compounds showed comparable or higher activity than that of ascorbic acid and trolox.