Dingjiang Liu
Schering-Plough
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Publication
Featured researches published by Dingjiang Liu.
Protein Science | 2003
Dingjiang Liu; William T. Windsor; Daniel F. Wyss
The NS3 helicase of the hepatitis C virus (HCV) unwinds double‐stranded (ds) nucleic acid (NA) in an NTP‐dependent fashion. Mechanistic details of this process are, however, largely unknown for the HCV helicase. We have studied the binding of dsDNA to an engineered version of subdomain 2 of the HCV helicase (d2ΔNS3h) by NMR and circular dichroism. Binding of dsDNA to d2ΔNS3h induces a local unfolding of helix (α3), which includes residues of conserved helicase motif VI (Q460RxxRxxR467), and strands (β1 and β8) from the central β‐sheet. This also occurs upon lowering the pH (4.4) and introducing an R461A point mutation, which disrupt salt bridges with Asp 412 and Asp 427 in the protein structure. NMR studies on d2ΔNS3h in the partially unfolded state at low pH map the dsDNA binding site to residues previously shown to be involved in single‐stranded DNA binding. Sequence alignment and structural comparison suggest that these Arg–Asp interactions are highly conserved in SF2 DEx(D/H) proteins. Thus, modulation of these interactions by dsNA may allow SF2 helicases to switch between conformations required for helicase function.
Magnetic Resonance in Chemistry | 2004
Yu-Sen Wang; Dingjiang Liu; Daniel F. Wyss
Journal of the American Chemical Society | 2004
Daiwen Yang; Yu Zheng; Dingjiang Liu; Daniel F. Wyss
Journal of Molecular Biology | 2001
Dingjiang Liu; Yu-Sen Wang; Jennifer J. Gesell; Daniel F. Wyss
Journal of Biomolecular NMR | 2003
Dingjiang Liu; Yu-Sen Wang; Daniel F. Wyss
Journal of Biomolecular NMR | 2003
Dingjiang Liu; Yu-Sen Wang; Daniel F. Wyss
Protein Engineering | 2001
Jennifer J. Gesell; Dingjiang Liu; Vincent S. Madison; Thomas Hesson; Yu-Sen Wang; Patricia C. Weber; Daniel F. Wyss
Journal of Biomolecular NMR | 2004
Dingjiang Liu; Yu-Sen Wang; Jennifer J. Gesell; Eileen Wilson; Brian M. Beyer; Daniel F. Wyss
Journal of Biomolecular NMR | 2001
Dingjiang Liu; Daniel F. Wyss
Journal of Biomolecular NMR | 2000
Dingjiang Liu; Daniel F. Wyss