Du Peige

Synthesis of vitcamphor derivatives of camphor and its preliminary anti-inflammatory activity

The vitcamphor was synthesized from camphor as starting materials via the bromination, followed by esterification, reduction, hydrolisis and oxidation. The structure of the target compound was confirmed by 1H NMR and 13C NMR, and the overall yield was increased from 6% to 12%. The purity of the tide compound was over 96 %. The anti-inflammatory activity of vitcamphor was determined by vascular permeability of mice and rat carrageenin induced edema model. The vitcamphor in used doses exhibited remarkable inhibitory effect on histamine induced increase of vascular permeability and significantly reduced the paw swelling.

Studies on the effects of corn polysaccharide and silkworm pupa to diabetes rats for decreasing blood glucose

Objective: To explore the effects of decreasing blood glucose of extracts of corn and the pupa to diabetic rats induced by STZ. Method: The mixture of corn and the pupa is extracted by water lifting reduction. ICR rats were intraperitoneally injected of STZ 40 mg/Kg, animal model of diabetes was prepared; to observe fasting blood glucose levels (FBG) of mice with glucose oxidase; to observe the serum cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL) and low-density lipoprotein (LDL) levels of all the animals with automatic biochemical analyzer; to observe serum insulin (INS) levels of mice with radioimmunoassay; Results: 1. Multiple low dose Intraperitoneal injection of STZ can lead to the model of diabetic mice close to the features of human disease. After modeling, diabetic mice have appeared typical symptoms of diabetes.2. Corn polysaccharide can alleviate the typical symptoms of diabetic mice, reduce blood sugar, improve lipid metabolism, advance insulin secrete of diabetic mice.

Protective effects of exenatide on RINm5f cell injury induced by palmitate

In this study, in vitro cultured islet RINm5f cells were used to observe effects of exenatide on the apoptosis induced by palmitate and explore its mechanism. MTT, AO/EB bi-label assay, flow cytometry and laser confocal microscope were applied for the observation of the cell proliferation, survival rate, apoptosis rate and morphological change. The results confirmed that the survival rate of cultured rat islet RINm5f cells treated with 250 μM palmitate for 24h was lower. Compared with the control group, the survival rate was higher, the apoptosis rate was lower and died cells and necrotic cells were less in exenatide groups. These facts indicate that exenatide can promote cell proliferation and improve rat islet cell apoptosis induced by palmitate, and promoting pancreatic B cell growth, increasing B cell volume, improving and restoring B cell function should be a new strategy used for the treatment of diabetes.

Cloning of HSA-GHGKHKNK fusion protein cDNA and its experssion in Pichia pastoris

GHGKHKNK octapeptide has tumoricidal potential mainly due to the amino acid residues of His-Gly-Lys motif inhibits the clone formation, adhesion and invasion of tumor cells. However, its clinical application is limited by its short half-life time in vivo. We used human serum albumin (HSA) fusion technology to fuse GHGKHKNK octapeptide and HSA to prolong half-life time of GHGKHKNK octapeptide and increase its stability. the GHGKHKNK — HSA fusion protein gene was cloned into the secretor type expression vector pPICZaC and subsequently expressed in Pichia pastoris. The supernatant fusion protein was detected by SDS-PAGE and purified with Blue Sepharose 6 Fast Flow chromatography. The clone formation rate of melanoma B16-F10 cell strain and the effect of the octapetide on the expression level of LN-R, ICAM-1 in melanoma B16-F10 cell strain were measured. Results suggested that infusion reaction between GHGKHKNK octapeptide and HSA did not destroy biologic activity of GHGKHKNK octapeptide.

Impact of amino acid substitutions in domain 5 of high molecular weight kininogen on suppression of breast cancer cells invasion

Domain 5 (D5) of high molecular weight kininogen represent significant anti-invasion properties towards tumor cells, and His-Gly-Lys (HGK) in D5 is the core motif for inhibition of invasion. In an attempt to identify the core amino acid of HGK in the process of tumor invasion, PCR-mediated site-directed mutagenesis technology was use to generate 3 mutant constructs, pGEX-4T-1/D5 (H485A), pGEX-4T-1/D5 (G486A) and pGEX-4T-1/D5 (K487A). These mutants fusion protein was expressed in E. coli. We further detect the effect of natural D5H and the mutants on tumor invasion and adhesion, and the expression level intracellular adhesion molecule-1 (ICAM-1) in human breast cancer cells. Our study demonstrates that Lys of HGK at 487 sit plays an important role in invasion inhibition. The results also provide a potent tool to clarify the molecular mechanisms involved in D5 invasion inhibition.