Sun Jingbo

Determination of isoniazid and isonicotinic acid contents in tablets by HPLC

A HPLC method for the determination of the content of Isoniazid and Isonicotinic acid in Isoniazid Tablets was established. The determination was performed on SHIM-PACK VP-ODS C<inf>18</inf> column. The mobile phase consisted of (40:60) methanol-water (0.2%Docusate Sodium) at a flow rate of 0.8ml·min⁻¹. The UV detection wavelength was set at 261nm and the sample size was 5μL. The linear range of isoniazid and isonicotinic acid were 10∼1000μg·mL⁻¹ (r=0.9998) and 2∼24μg·mL⁻¹ (r=0.9994) respectively, which showed that there was a good linear relationship in isoniazid test concentration and the isonicotinic acid test concentration respectively. The average recoveries were 99.57% (RSD=0.64%) and 98.96% (RSD=1.15%), respectively. The results demonstrated that the repeatability of the method was satisfactory and the precision of the method was satisfactory. The established method is simple, rapid and accurate, and suitable for the quality control of Isoniazid tablets. It is significant to the overall qualitative control of Isoniazid Tablets.

Comparative analysis of components in the volatile oil of Saposhnikovia divaricata collected from different areas at two different harvest times

In this pater, the primary constituents in volatile oils of Saposhinikavia divarica(S.D.) collected in different harvest times were analyzed and identyfyed. Water vapor distillation was used to extract the volatile of S.D. and GC-MS was used to analyze and identify the chemical compinents of the volatile oils. As a result, the type of chemical constitents and their contents in the sample volatile oils were significantly different. 32 of 122 compounds exsited in all samples and most of them were terpenoids and chained aliphatic. The content of volatile oil in wild S.D. collected in November were higher than that in August, whereas that in the planted S.D. was higher in August than in November. It is the first time that the differce in volatile oil content between the wild and the planted S.D. is proposed. The research provide important evidences for the future research on the pharmacology, planting, quality control, and the development and utilization on the herbal medicinal resources in Saposhnikovia divaricata.