Yuan Guangxin

Determination of isoniazid and isonicotinic acid contents in tablets by HPLC

A HPLC method for the determination of the content of Isoniazid and Isonicotinic acid in Isoniazid Tablets was established. The determination was performed on SHIM-PACK VP-ODS C<inf>18</inf> column. The mobile phase consisted of (40:60) methanol-water (0.2%Docusate Sodium) at a flow rate of 0.8ml·min⁻¹. The UV detection wavelength was set at 261nm and the sample size was 5μL. The linear range of isoniazid and isonicotinic acid were 10∼1000μg·mL⁻¹ (r=0.9998) and 2∼24μg·mL⁻¹ (r=0.9994) respectively, which showed that there was a good linear relationship in isoniazid test concentration and the isonicotinic acid test concentration respectively. The average recoveries were 99.57% (RSD=0.64%) and 98.96% (RSD=1.15%), respectively. The results demonstrated that the repeatability of the method was satisfactory and the precision of the method was satisfactory. The established method is simple, rapid and accurate, and suitable for the quality control of Isoniazid tablets. It is significant to the overall qualitative control of Isoniazid Tablets.

Rapid and simultaneous determination of rutin, chlorogenic acid and quercetin in mulberry folium leaf by capillary electrophoresis

To improve a capillary electrophoresis method used for the rapid and simultaneous separation and determination of rutin, chlorogenic acid and quercetin. The capillary column without coating (50μm×56cm, the used length: 47.5cm) was used, the solution of 12mM sodium tetraborate pentahydrate (pH 9.0) containing 15% methanol was taken as the running buffer and the temperature was 25 _. The linear relationship, recover rate and repeatability of rutin, chlorogenic acid and quercetin were better when they were detected at wave length 350nm. The method we used should be accurate and suitable for the quality control of mulberry folium leaf which is a crude drug.

Establishment of mink heart identification method based on mitochondrial cytochrome b gene and development of its detection kit

Abstract In this study, the mink heart mitochondrial DNA was used as the target gene to design the specific primers of mink heart mtDNA Cytb, the extraction and detection reagents of mink heart DNA were developed using DNA fingerprinting technique, and the specificity, reproducibility, and stability of the reagents were investigated. Molecular cloning and sequencing technique were used to clone the standard substance of mink heart DNA detection, then a DNA fingerprint detection method of mink heart and the quality standard for mink heart were established, and a DNA detection kit of mink heart was developed. The results showed that the structure of DNA extracted from mink heart by self-developed reagents was complete, and both the concentration and purity of DNA were high. A specific amplification band of the original mink samples was found at 337 bp. The sequence of mink heart DNA was consistent with that of mink heart mtDNA specific fingerprint region. The mink heart DNA fingerprint identification method established, in this study, is accurate and reliable, and the procedure of the developed DNA kit is easy, the results obtained using this kit is stable and the method is suitable for popularization and application.

Research for ultramicrofingerprint of Semen Cuscutae

The paper reports an identification method of Semen Cuscutae. The ultramicofingerprint features of Semen Cuscutae is probed with scanning electron microscope and other two different methods, which researched on standard sample and random samples. There is an obvious difference between Semen Cuscutae and its garble by scanning electroscopic according to our research. This method can be used for identification of Semen Cuscutae conveniently, quickly and accurately.

Rapid determination of lignans in Schisandra Chinensis and Schisandra sphenanthera by micellar electrokinetic capillary chromatography

A simple and rapid micellar electrokinetic capillary chromatography (MEKC) method has been developed for the simultaneous determination of six lignans, schizandrin, Gomisin A, schisantherin A, deoxyschizandrin, schizandrin B and schizandrin C, in fruits of S. chinensis and S. sphenanthera. The key factors for separation and determination were studied and the best analysis conditions were obtained, in the buffer of 10 mM phosphate-37.5 mM SDS-35% v/v acetonitrile (pH 8.0) at the separation voltage of 28.0 KV and detection at 214 nm, the plant samples could be detected within 8.2 min. Analysis yielded good reproducibility (RSD between 1.19-2.28%) and good recovery (between 92.2-102.9%). The method is quick, sensitive, accurate and it could be applied to the quality control of S. chinensis and S. sphenanthera.