Dušan Hamar

Long-Term High-Level Exercise Promotes Muscle Reinnervation With Age

The histologic features of aging muscle suggest that denervation contributes to atrophy, that immobility accelerates the process, and that routine exercise may protect against loss of motor units and muscle tissue. Here, we compared muscle biopsies from sedentary and physically active seniors and found that seniors with a long history of high-level recreational activity up to the time of muscle biopsy had 1) lower loss of muscle strength versus young men (32% loss in physically active vs 51% loss in sedentary seniors); 2) fewer small angulated (denervated) myofibers; 3) a higher percentage of fiber-type groups (reinnervated muscle fibers) that were almost exclusive of the slow type; and 4) sparse normal-size muscle fibers coexpressing fast and slow myosin heavy chains, which is not compatible with exercise-driven muscle-type transformation. The biopsies from the old physically active seniors varied from sparse fiber-type groupings to almost fully transformed muscle, suggesting that coexpressing fibers appear to fill gaps. Altogether, the data show that long-term physical activity promotes reinnervation of muscle fibers and suggest that decades of high-level exercise allow the body to adapt to age-related denervation by saving otherwise lost muscle fibers through selective recruitment to slow motor units. These effects on size and structure of myofibers may delay functional decline in late aging.

Circulating MicroRNAs as Potential Biomarkers of Exercise Response

Systematic physical activity increases physical fitness and exercise capacity that lead to the improvement of health status and athletic performance. Considerable effort is devoted to identifying new biomarkers capable of evaluating exercise performance capacity and progress in training, early detection of overtraining, and monitoring health-related adaptation changes. Recent advances in OMICS technologies have opened new opportunities in the detection of genetic, epigenetic and transcriptomic biomarkers. Very promising are mainly small non-coding microRNAs (miRNAs). miRNAs post-transcriptionally regulate gene expression by binding to mRNA and causing its degradation or inhibiting translation. A growing body of evidence suggests that miRNAs affect many processes and play a crucial role not only in cell differentiation, proliferation and apoptosis, but also affect extracellular matrix composition and maintaining processes of homeostasis. A number of studies have shown changes in distribution profiles of circulating miRNAs (c-miRNAs) associated with various diseases and disorders as well as in samples taken under physiological conditions such as pregnancy or physical exercise. This overview aims to summarize the current knowledge related to the response of blood c-miRNAs profiles to different modes of exercise and to highlight their potential application as a novel class of biomarkers of physical performance capacity and training adaptation.

Atrophy/hypertrophy cell signaling in muscles of young athletes trained with vibrational-proprioceptive stimulation

Abstract Objective: To compare the effects of isokinetic (ISO-K) and vibrational-proprioceptive (VIB) trainings on muscle mass and strength. Methods: In 29 ISO-K- or VIB-trained young athletes we evaluated: force, muscle fiber morphometry, and gene expression of muscle atrophy/hypertrophy cell signaling. Results: VIB training increased the maximal isometric unilateral leg extension force by 48·1%. ISO-K training improved the force by 24·8%. Both improvements were statistically significant (P0·01). The more functional effectiveness of the VIB training in comparison with the ISO-K training was shown by the statistical significance changes only in VIB group in: rate of force development in time segment 0-50 ms (P<0·001), squat jump (P<0·05) and 30-m acceleration running test (P<0·05). VIB training induced a highly significant increase of mean diameter of fast fiber (+9%, P<0·001), but not of slow muscle fibers (−3%, not significant). No neural cell adhesion molecule-positive (N-CAM+) and embryonic myosin heavy chain-positive (MHC-emb+) myofibers were detected. VIB induced a significant twofold increase (P<0·05) of the skeletal muscle isoform insulin-like growth factor-1 (IGF-1) Ec mRNA. Atrogin-1 and muscle ring finger-1 (MuRF-1) did not change, but myostatin was strongly downregulated after VIB training (P<0·001). Peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) expression increased in post-training groups, but only in VIB reached statistical significance (+228%, P<0·05). Discussion: We demonstrated that both trainings are effective and do not induce muscle damage. Only VIB-trained group showed statistical significance increase of hypertrophy cell signaling pathways (IGF-1Ec and PGC-1α upregulation, and myostatin downregulation) leading to hypertrophy of fast twitch muscle fibers.

Physical exercise in Aging: Nine weeks of leg press or electrical stimulation training in 70 years old sedentary elderly people

Sarcopenia is the age-related loss of muscle mass and function, reducing force generation and mobility in the elderlies. Contributing factors include a severe decrease in both myofiber size and number as well as a decrease in the number of motor neurons innervating muscle fibers (mainly of fast type) which is sometimes accompanied by reinnervation of surviving slow type motor neurons (motor unit remodeling). Reduced mobility and functional limitations characterizing aging can promote a more sedentary lifestyle for older individuals, leading to a vicious circle further worsening muscle performance and the patients’ quality of life, predisposing them to an increased risk of disability, and mortality. Several longitudinal studies have shown that regular exercise may extend life expectancy and reduce morbidity in aging people. Based on these findings, the Interreg IVa project aimed to recruit sedentary seniors with a normal life style and to train them for 9 weeks with either leg press (LP) exercise or electrical stimulation (ES). Before and at the end of both training periods, all the subjects were submitted to mobility functional tests and muscle biopsies from the Vastus Lateralis muscles of both legs. No signs of muscle damage and/or of inflammation were observed in muscle biopsies after the training. Functional tests showed that both LP and ES induced improvements of force and mobility of the trained subjects. Morphometrical and immunofluorescent analyses performed on muscle biopsies showed that ES significantly increased the size of fast type muscle fibers (p<0.001), together with a significant increase in the number of Pax7 and NCAM positive satellite cells (p<0.005). A significant decrease of slow type fiber diameter was observed in both ES and LP trained subjects (p<0.001). Altogether these results demonstrate the effectiveness of physical exercise either voluntary (LP) or passive (ES) to improve the functional performances of aging muscles. Here ES is demonstrated to be a safe home-based method to counteract fast type fiber atrophy, typically associated with aging skeletal muscle.

Physiological Mechanisms of Post-Exercise Balance Impairment

This review focuses on investigations into postural sway response to different forms of exercise, and particularly those dealing with physiological mechanisms of post-exercise balance impairment. The findings of relevant studies combined with our research results show that postural sway response to exercise depends on its type, intensity, duration and intensity of proprioceptive stimulation. Differences in postural sway were also observed after exercise with different forms of muscle contraction and those with different activation of muscle fibres. In general, fatigue is associated with post-exercise balance impairment. Such an effect is usually a consequence of prolonged exercise. However, findings proved that after short-term intensive exercises, hyperventilation, rather than fatigue, is responsible for increased postural sway. Significant differences in balance impairment were also found after exercises that induced the same ventilation but with a different intensity of muscle contractions eliciting a different level of proprioceptive stimulation, such as calf rises versus jumps and cycling versus running. Thus, in addition to fatigue and hyperventilation, impairment of somatosensory inputs also very likely plays a role in increased post-exercise postural sway. Based on this literature review, possible physiological mechanisms of post-exercise balance impairment identified so far may be fatigue, hyperventilation, deterioration of functions of mechanoreceptors, proprioceptors, vestibular apparatus and visual cues, muscle damage, dehydration, hyperthermia and dizziness. These findings can be explored in a more practical setting in the field of sport, clinical medicine and rehabilitation.

Power Outputs in the Concentric Phase of Resistance Exercises Performed in the Interval Mode on Stable and Unstable Surfaces

Abstract Zemková, E, Jeleň, M, Kováčiková, Z, Ollé, Z, Vilman, T, and Hamar, D. Power outputs in the concentric phase of resistance exercises performed in the interval mode on stable and unstable surfaces. J Strength Cond Res 26(12): 3230–3236, 2012—The study compares power outputs in the concentric phase of chest presses and squats performed in the interval mode on stable and unstable surface, respectively. A group of 16 physical education students performed randomly on different days 6 sets of 8 repetitions of (a) chest presses on the bench and Swiss ball, respectively, and (b) squats on stable support base and Bosu ball, respectively, with 2 minutes of rest period between sets. The exercises were performed with previously established 70% of 1 repetition maximum under stable conditions. A PC-based system FiTRO Dyne Premium was used to monitor force and velocity and to calculate power. The results showed significantly lower power outputs when resistance exercises were performed on an unstable than a stable support base. In the initial set, mean power in concentric phase of lifting decreased more profoundly under unstable than under stable conditions during both chest presses (13.2 and 7.7%, respectively) and squats (10.3 and 7.2%, respectively). In the final set, the reduction rates of mean power in the concentric phase of chest presses were significantly (p < 0.05) greater on the Swiss ball than on the bench (19.9 and 11.8%, respectively). On the other hand, there were no significant differences in decline of mean power in the concentric phase of squats on the Bosu ball and on stable support base (11.4 and 9.6%, respectively). It may be concluded that power outputs during resistance exercises is more profoundly compromised under unstable than under stable conditions, and this effect is more evident for barbell chest presses on the Swiss ball than for barbell squats on the Bosu ball. These findings have to be taken into account when instability resistance exercises are implemented into the training program, namely, for sports that require production of maximal force in short time.

Physical activity in elderly

Aging is a multifactorial irreversible process associated with significant decline in muscle mass and neuromuscular functions. One of the most efficient methods to counteract age-related changes in muscle mass and function is physical exercise. An alternative effective intervention to improve muscle structure and performance is electrical stimulation. In the present work we present the positive effects of physical activity in elderly and a study where the effects of a 8-week period of functional electrical stimulation and strength training with proprioceptive stimulation in elderly are compared.

Weight Lifted and Countermovement Potentiation of Power in Concentric Phase of Unstable and Traditional Resistance Exercises

The study evaluates the effect of weight lifted on power in the concentric phase of resistance exercises on stable and unstable surfaces. A group of 19 fit men performed randomly on different days 3 reps of (a) barbell chest presses on the bench and Swiss ball, and (b) barbell squats on stable base and BOSU ball. Exercises were performed without and with countermovement (CM) using maximal effort in concentric phase. Initial weight of 20 kg was increased by 10 kg or 5 kg (at higher loads) up to at least 85% of previously established 1RM under stable conditions. Results showed no significant differences in mean power in the concentric phase of stable and unstable CM chest presses at lower weights lifted (from 20 to 50 kg). However, its values were significantly higher during chest presses on the bench than on Swiss ball while lifting higher weights (from 60 to 90 kg). Similarly, mean power in the concentric phase of squats was significantly higher on stable base than on BOSU ball at higher weights lifted (from 60 to 90 kg). Though a set of data showed significant differences, the effect sizes≤0.7 suggest no practically meaningful differences. It may be concluded that unstable base compromises the power in the concentric phase of resistance exercises, however, only at higher weights lifted.

Universal linear motor driven Leg Press Dynamometer and concept of Serial Stretch Loading

Paper deals with backgrounds and principles of universal linear motor driven leg press dynamometer and concept of serial stretch loading. The device is based on two computer controlled linear motors mounted to the horizontal rails. As the motors can keep either constant resistance force in selected position or velocity in both directions, the system allows simulation of any mode of muscle contraction. In addition, it also can generate defined serial stretch stimuli in a form of repeated force peaks. This is achieved by short segments of reversed velocity (in concentric phase) or acceleration (in eccentric phase). Such stimuli, generated at the rate of 10 Hz, have proven to be a more efficient means for the improvement of rate of the force development. This capability not only affects performance in many sports, but also plays a substantial role in prevention of falls and their consequences. Universal linear motor driven and computer controlled dynamometer with its unique feature to generate serial stretch stimuli seems to be an efficient and useful tool for enhancing strength training effects on neuromuscular function not only in athletes, but as well as in senior population and rehabilitation patients.

Enhancement of Peak and Mean Power in Concentric Phase of Resistance Exercises

Abstract Zemková, E, Jeleň, MN, Kováčiková, ZC, Ollé, G, Vilman, TS, and Hamar, DS. Enhancement of peak and mean power in concentric phase of resistance exercises. J Strength Cond Res 28(10): 2919–2926, 2014—The study compares the differences in peak and mean power of concentric-only and countermovement resistance exercises (&Dgr;P) with different weights. A group of 27 fit men randomly performed 3 repetitions of either barbell bench presses or barbell squats on different days. The initial weight of 20 kg was increased by 10 or 5 kg (at higher loads) up to at least 85% of a previously established 1 repetition maximum (1RM). A computer-based system FiTRO Dyne Premium was used to monitor force and velocity and to calculate power. The peak values and mean values of power during the entire concentric phase of lifting and during the acceleration phase were analyzed. Results showed that maximal &Dgr;P calculated from the peak and mean values in the acceleration phase of bench presses was achieved at lower weights (118.4 ± 19.0 W at 47% of 1RM and 116.2 ± 15.3 W at 48% of 1RM, respectively) than the one calculated from mean values in the entire concentric phase of lifting (114.8 ± 14.8 W at 57% 1RM). Likewise, maximal &Dgr;P calculated from the peak and mean values in the acceleration phase of squats was achieved at lower weights (127.7 ± 20.4 W at 67% of 1RM and 124.3 ± 22.1 W at 69% of 1RM, respectively) than the one calculated from the mean values in the entire concentric phase of lifting (125.0 ± 19.2 W at 77% of 1RM). This fact has to be taken into account when training efficiency is evaluated, namely, in sports requiring the production of maximal force in a short time.