Eddy Afrianto

EXTRACTION OF SNAKEHEAD FISH [Ophiocephalus striatus (Bloch, 1793)] INTO FISH PROTEIN CONCENTRATE AS ALBUMIN SOURCE USING VARIOUS SOLVENT

Study aimed to determine the optimum solvent for extraction of soluble protein (albumin) and identify the chemical composition of snakehead fish protein concentrate. The method was experimental while the treatments were the variation of solvents: distilled water, HCl 0.1 M, and NaCl 0.9 %. Soluble protein (albumin) and yield parameters analyzed by using completely randomized design (CRD) which consist three treatments and four replications, the other parameters were described descriptively. The result showed that the highest soluble protein (albumin) (7.65 %) was produced by HCl 0.1 M solvent with 2.55 % yield, 10.76 % dry basis moisture content, 63.78 % total protein content, and 2.54 % fat content.

The addition of nanochitosan suspension as filler in carrageenan-tapioca biocomposite film

This research aimed to investigate the effect of nanochitosan (CSNPs) suspension by beads milling method as filler in carrageenan-tapioca biocomposite film. In addition, the antibacterial activity of CSNPs as filler with two food pathogenic bacteria, Staphylococcus aureus and Escherichia coli and then influence of nano fillers for appearance of films were observed. The incorporation of CSNPs suspension with 0.5, 1, 1.5 and 2 (%v/v) in carrageenan-tapioca film exhibited antibacterial activity againts both bacteria. CSNPs had slightly higher antimicrobial activity against E. coli aureus compared to S. aureus at all concentrations due to different mechanisms. Therefore, the best antimicrobial activity was obtained from 1 wt%. Furthermore the best antimicrobial activity was characterized by means of the thickness and transparency. The result showed that the thickness of film was 0.059 mm and the transparency was 87.88. It was concluded that the incorporation of CSNPs suspension 1 wt% in carrageenan-tapioca composite film is suitable for developing active packaging.This research aimed to investigate the effect of nanochitosan (CSNPs) suspension by beads milling method as filler in carrageenan-tapioca biocomposite film. In addition, the antibacterial activity of CSNPs as filler with two food pathogenic bacteria, Staphylococcus aureus and Escherichia coli and then influence of nano fillers for appearance of films were observed. The incorporation of CSNPs suspension with 0.5, 1, 1.5 and 2 (%v/v) in carrageenan-tapioca film exhibited antibacterial activity againts both bacteria. CSNPs had slightly higher antimicrobial activity against E. coli aureus compared to S. aureus at all concentrations due to different mechanisms. Therefore, the best antimicrobial activity was obtained from 1 wt%. Furthermore the best antimicrobial activity was characterized by means of the thickness and transparency. The result showed that the thickness of film was 0.059 mm and the transparency was 87.88. It was concluded that the incorporation of CSNPs suspension 1 wt% in carrageenan-tapioca co...