Eliane Carvalho de Vasconcelos

Environmental risk assessment of antibiotics: an intensive care unit analysis.

Hospital effluents have been usually known by the microbiological pollution they cause, but only recently they have been considered a significant source of aquatic environmental pollution due to the presence of medicines in these effluents. In this context, an environmental risk assessment (ERA) is presented for the most used intravenous antibiotics in the Intensive Care Unit (ICU) of a hospital in Curitiba (Brazil). The amount of antibiotics used in the ICU was evaluated during 18months (June 2006 until November 2007), in order to calculate the Predicted Environmental Concentration (PEC1). Antibiotic excretion data (on its original form) and the removal of the selected drugs in the sewage treatment plants based on the activated sludge system were used to calculate, respectively, PEC2 and PEC2r. The Predicted No-Effect Concentration (PNEC) of pharmaceuticals was also considered to assess the environmental risk by calculating the PEC/PNEC ratios. All PECs were 1ngL(-1). The worst-case PEC estimations (PEC1 and PEC2) were observed for sodic ceftriaxone, sodic cefazolin, meropenem, ampicillin, cefepime and sodic piperacillin. PEC/PNEC ratios showed that, given the present pattern of usage, high aquatic environmental risk is expected for these antibiotics. Further studies should be carried out to elucidate their contribution to increasing antimicrobial multi-drug-resistant species.

Thermoanalytical and starch content evaluation of cassava bagasse as agro-industrial residue

As fracoes nutricionais bem como as propriedades termicas e outras analises sao essenciais para a industria de alimentos e suas aplicacoes O bagaco de mandioca e um importante residuo agroindustrial e seu teor de amido foi avaliado por dois metodos alternativos. A caracterizacao por analise termica e microscopia ajudou na compreensao de como a hidrolise digere a fracao amilacea do bagaco de mandioca, O ponto de fusao foi de 170oC, a analise termogravimetrica (TG) mostrou apos a perda de umidade do material, duas principais perdas de massa em todas as amostras analisadas. Os resultados sugerem que a hidrolise enzimatica e menos eficiente na conversao total de amido no bagaco de mandioca. No entanto, o uso de acido sulfurico degradou ate mesmo a parcela fibrosa do material, afetando as condicoes de analise.

Identification and quantification of monomers released from dental composites using HPLC

The aim of this study was to detect and quantify the main residual monomers released from composites, using high performance liquid chromatography (HPLC). Discs were made with dental composites (Herculite XRV, Tetric Ceram and Filtek Z250) and immersed in deionized water at 37oC for 28 days, with water changes in 1, 7, 14 and 21 days. The mean concentration of residual monomers were subject to the Kruskal-Wallis test (p<0.05). Tetric Ceram exhibited significantly higher concentrations of leached monomers. Bis-GMA was the monomer released in lower concentrations for all the materials. There was no statistical difference between the amounts of TEGDMA and UDMA. Most of the monomers demonstrated maximal concentration at the 7-day period. The HPLC analysis identified Bis-GMA, TEGDMA and UDMA in detectable quantities for all the tested composites.

Environmental Risk Assessment (ERA) of diclofenac and ibuprofen: A public health perspective

Non-steroidal anti-inflammatory drugs (NSAIDs) have been widely used in human and veterinary medicine, representing potential aquatic environmental contamination. This study aimed to perform an Environmental Risk Assessment (ERA) of NSAIDs diclofenac (Dic) and ibuprofen (Ibu) in cities of the state of Paraná, Brazil, over the course of three years, by using available data from the Brazilian Public Health System. The environmental risk (ER) was assessed by employing the European Medicines Agency (EMeA) approach, and predicted environmental concentrations (PECs) were calculated. The refined PECs considered the drug metabolism and the excretion data, and also the sewage treatment plant removal rates of biological filters and activated sludge processes to define environmental scenarios. References to the predicted no effect concentration (PNEC) for these pharmaceuticals were considered, and the PEC/PNEC ratio was calculated; ratio values ⩾ 1 suggested an ER. Environmental risk was conducted on several cities, and the lack of an adequate sanitation system in the majority of Paraná cities forecasts a significant concern with the exposure to possible environmental damages in those cities. The high PEC/PNEC ratios in several cities showed that current usage patterns of these drugs constitute an environmental issue in need of resolution by health and environmental authorities.

Comparative behavior studies of cinnamic acid using isothermal and nonisothermal kinetic methods

In this work, a thermal behavior study of cinnamic acid was made using isothermal and nonisothermal kinetics methods. Thermogravimetric curves were obtained to determine the kinetic parameters of its decomposition process. Cinnamic acid was melted at TPeak = 135.5°C and the decomposition started at 160°C. The obtained activation energy was 71.13 kJ/mol and 65.77 kJ/mol for isothermal and nonisothermal methods, respectively. The decomposition kinetics for both methods occurs at a constant rate, is of zero order, and is independent of the concentration of the reactants.

Adsorption of pharmaceuticals in water through lignocellulosic fibers synergism

The contamination of water from disposal of drugs is an emerging problem due to their consequences on trophic webs. This study evaluated the ability of sugarcane and coconut fiber to reduce water toxicity contaminated by pharmaceuticals. The toxicity of solutions containing pharmaceuticals was studied by bioassay using Allium cepa, before and after filtration of contaminated water. The coconut and sugarcane fiber have not been satisfactory in reducing toxicity when tested separately. Despite no induction of chromosomal aberrations, our study found a reduction of the mitotic index. The mixture of fibers showed better results providing total reduction of toxicity, in addition to maintenance in the mitotic index and induction of chromosome aberrations. The interaction between fibers and drugs was confirmed by Thermogravimetry and Differential Thermal Analyses (TG/DTA) which presented differences in profile between the fibers before and after adsorption. The mixture of coconut and sugarcane proved viable for reduction of toxicity in contaminated water by a mixture of pharmaceuticals.