Endang Prangdimurti

Analysis of Blood Glucose and Plasma Insulin Levels of Tea and Cinnamon Tea Drink against People with Diabetes Mellitus Type 2

Diabetes Mellitus is a chronic disease characterized by increased blood glucose levels as a result of a lack of insulin or the body is unable to use insulin. One of the plants that have the potential to lower blood glucose levels of diabetics mellitus is cinnamon and tea. Testing the effect of cinnamon tea and tea to the insulin that play a role in decreasing blood glucose levels. Therefore, to investigate the effect of cinnamon tea and tea drinks is the most effective on blood glucose levels, body weight, and plasma insulin levels respondents diabetes mellitus type 2. Treatments consisted of a control group and two treatment groups. The control group is a negative control (diabetes mellitus without any treatment). The treatment group consisted of treatment of tea and cinnamon tea. Respondents who have diabetes mellitus type 2 measured levels of blood glucose, plasma insulin levels against all test groups. Analysis of blood glucose using a glucometer showed changes in the results for each respondent is different treatment; treatment of cinnamon drink had a decrease in the blood glucose level is different from the tea drink treatment experienced a significant rise in blood glucose. Analysis of insulin using ELISA showed changes in work procedures different results; treatment cinnamon increased insulin levels while the tea treatment decreased insulin levels. This study shows that the influence of cinnamon drink to the reduction of blood glucose and insulin levels occurred in respondents with diabetes mellitus type 2.

Extraction of Squid (Photololigo Duvaucelii) Myofibrillar and Sarcoplasmic Proteins to Create A Skin Prick Test Reagent In The Diagnosis of Food Allergy

The major and minor allergens in fish and shellfish that have been identified are broadly spread in the myofibrillar and sarcoplasmic proteins. This study aimed to create the SPT reagents of squid myofibrillar and sarcoplasmic proteins to improve sensitivity and specificity diagnosis. Myofibrillar protein fraction was composed of 11 protein bands with the molecular weights of 18.2-108.4 kDa and sarcoplasmic protein fraction was composed of 13 protein bands with the molecular weights of 9.5-119.3 kDa. The allergenic myofibrillar protein has the molecular weights of 8.9-122.7 kDa while the allergenic sarcoplasmic protein has the molecular weights of 11.8-143.9 kDa. Each allergen extract was formulated into a SPT reagent met the requirements of the European Pharmacopoeia Monograph on Allergen Products 7 (2010:1063) for the parameters of moisture content, protein content, sterility and microbiology. A SPT to the respondents indicated that the sensitivity value of squid myofibrillar reagent was 86% and squid sarcoplasmic reagent was 64% with a negative error rate for each myofibrillar and sarcoplasmic reagents was 14% and 36%, respectively. The result diagnosis of squid myofibrillar and sarcoplasmic would generate sensitivity value at 100%. The separation of squid proteins into myofibrils and sarcoplasm for SPT could improve its sensitivity value and lowered the negative misdiagnosis. The specificity of each myofibrillar and sarcoplasmic proteins was 100% with an error rate of positive diagnosis occurrence at 0%.