Fransiska Rungkat Zakaria

Novel probiotic Enterococcus faecium IS-27526 supplementation increased total salivary sIgA level and bodyweight of pre-school children: a pilot study.

Enterococcus faecium IS-27526 is a novel probiotic isolated from dadih, an Indonesian traditional fermented buffalo milk. A 90 days randomized double-blind placebo-controlled study of pre-post trial was conducted in pre-school children with two groups, placebo and probiotic group. Ultra High Temperature low fat milk was used as a carrier in each group. The aims of this study were to investigate the effect of E. faecium IS-27526 in milk on humoral immune response and on bodyweight of pre-school children. Total serum IgA and total salivary sIgA were measured by sandwich ELISA. The bodyweight of young children was measured. The results showed that total serum IgA did not significantly increase in the probiotic group compared with the placebo group. Total salivary sIgA level and the bodyweight significantly increased (p < 0.05) in probiotic groups compared to the placebo. Changes of total salivary sIgA level were significantly higher in underweight children supplemented with probiotic. Weight gain was observed significantly in children with normal bodyweight supplemented with probiotic. Neither mortality nor weight loss was recorded throughout the study. Taken together, novel probiotic E. faecium IS-27526 has significant positive effects on humoral immune response, salivary sIgA, in underweight pre-school children, and on weight gain of pre-school children.

Efek suplementasi Zn terhadap status imun wanita premenopause yang diintervensi dengan minuman berisoflavon

The research was conducted to find out the effect of Zn supplement to immune status of premenopausal women intervented with isoflavoned drinking. Respondents were 22 women, more than 40 year of age. They were divided into two groups, i.e. 11 women intervented with isoflavone, and other 11 women intervented with isoflavone and 8 mg Zn. The activities of SOD, catalase and GPX were determined by spectrophotometer, thymulin levels by ELISA, whereas Zn levels by AAS. Result showed that Zn had significantly increased SOD lymphocyte activities (p=0.002) and thymulin plasma (p=0.011). Zn had increased catalase (p=0.103) and GPX (p=0.322) as well, but Zn plasma had decreased (0.163). It was indicated that Zn had improved the immune status by increasing lymphocyte and thymus cells activities

Analisis Persepsi Konsumen terhadap Produk Minyak Sawit Merah Sebagai Minyak Kesehatan (Studi Kasus: Perumahan Ciomas Permai, Bogor)

Product of red palm oil is a new product which is still not marketed yet insufficient quantity so that it is not recognized by many people . Because of that, it needs promotion about the benefit and quality for the consumer. The approach in this research is market or selling the product of red palm oil to the respondent through direct marketing ( door to door ), and at the same time do the analysis of consumer’s perception to the product of red palm oil. In general the aim of this research is to know consumer’s perception in getting decision of buying and strategy alternative which can be done in marketing of red palm oil in Perumahan Ciomas Permai, Bogor. Location of this research is in Perumahan Ciomas Permai, Blok B Desa Ciapus Kecamatan Ciomas Kabupaten Bogor. The method of this research is case study with descriptive and quantitative analysis with multiple regression. The processing and data analysis were done with application of Microsoft Excel, which is shown in the form of tabulation and correlation matrix and analysis multiple regression linear with counting use computer program SPSS. The number of respondent were 35 people who represent middle household. Based on the research, through direct marketing and consumer’s analysis, it was found that there were four factors which influence the process of buying decision red palm oil namely price, color, content of nutrient and the packaging. The strategy of marketing which can be done for the product of red palm oil is maintain and improve the quality of red palm oil (the hygienic control, quality, purity, color and nutrient content), giving a discount in a certain sales, promotion by distributing brochure and leaflet, service of delivery and guarantee the continuity of red palm oil availability.

Effect of Zn Supplemented to Immune Status Premenopausal Women Intervented with Isoflavoned Drinking

Sperm collected from cauda epididymis is a source of male gametes. The purposes of this study was to evaluate an quality of frozen-thawed sperm of garut ram which was collected from cauda epididymis and cryopreserved with modified Tris extender, i.e: Tris extender (control, KT), Tris extender + 60 mM lactose (LS), and Tris extender + 60 mM lactose + 0.05% glutathione (GL). Quality of collected sperm including concentration, motility, live sperm, abnormality, cytoplasmic droplet, intact acrosomal cap (IAC), and intact plasma membrane (IPM) were evaluated. Results showed that mean of sperm concentration, percentages of motility, live sperm, abnormality, cytoplasmic droplet, IAC, and IPM of fresh epididymal sperm were 13,993.33 million/ml, 70.83, 82.83, 10.83, 8.5, 85.83, and 81.33%, respectively. Sperm quality after equilibration for LS and GL were significantly (PExperiments were conducted on the morphology, fertilization and embryo development rate of vitrified ovine oocytes matured in vitro. Three vitrification solutions were used for vitrification. PBS supplemented with 1% BSA, 30% ethylene glycol was added by one of three different sucrose concentrations, 1.00 M (VS1), 0.50 M (VS2), and 0.25 M (VS3). The results showed that the percentages of normal vitrified oocytes after warming were 78 and 63% in VS1 and VS2, respectively, which was significantly higher as compared for VS3. The fertilization rates were 59 and 66% in VS1 and VS2, respectively, which were also significantly higher as compared with VS3 (35%). Zygote viability after 18 h was 57; 43; and 40%, for VS1,VS2, and VS3, respectively, which was not significantly different. The incidence of polyspermic penetration increased with increasing sucrose concentration, i.e 23, 11, and 9% in VS1, VS2, and VS3, respectively, as compared with unvitrified oocytes (4%). The cleavage rate of vitrified oocytes in VS1 was 13.2% which was significantly lower (p

Analysis of Blood Glucose and Plasma Insulin Levels of Tea and Cinnamon Tea Drink against People with Diabetes Mellitus Type 2

Diabetes Mellitus is a chronic disease characterized by increased blood glucose levels as a result of a lack of insulin or the body is unable to use insulin. One of the plants that have the potential to lower blood glucose levels of diabetics mellitus is cinnamon and tea. Testing the effect of cinnamon tea and tea to the insulin that play a role in decreasing blood glucose levels. Therefore, to investigate the effect of cinnamon tea and tea drinks is the most effective on blood glucose levels, body weight, and plasma insulin levels respondents diabetes mellitus type 2. Treatments consisted of a control group and two treatment groups. The control group is a negative control (diabetes mellitus without any treatment). The treatment group consisted of treatment of tea and cinnamon tea. Respondents who have diabetes mellitus type 2 measured levels of blood glucose, plasma insulin levels against all test groups. Analysis of blood glucose using a glucometer showed changes in the results for each respondent is different treatment; treatment of cinnamon drink had a decrease in the blood glucose level is different from the tea drink treatment experienced a significant rise in blood glucose. Analysis of insulin using ELISA showed changes in work procedures different results; treatment cinnamon increased insulin levels while the tea treatment decreased insulin levels. This study shows that the influence of cinnamon drink to the reduction of blood glucose and insulin levels occurred in respondents with diabetes mellitus type 2.

Allergic reactivity of bambara groundnut (Vigna subterranea) proteins

ABSTRACT All food proteins have a potential to be allergic for some people. Nuts and legumes are some of the main food allergens, which are consumed by large numbers of people. Several researches had been reported about the allergenicity of peanut, soybean, tree nuts, but not of the bambara groundnut (Vigna subterranea), one of the legumes consumed largely by the population in Indonesia. The reported study aimed to isolate bambara groundnut protein and to analyze its allergic reactivity. The protein isolate reactivity was tested by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), enzyme-linked immunosorbent assay (ELISA) and immunoblotting, involving six peanut and soybean allergic human sera. ELISA analysis showed that the bambara groundnut protein had allergenic proteins. Furthermore, immunoblotting analysis revealed that serum IgE from the donors could bind to individual allergens to various degrees, demonstrating the specificity of IgE reactivity. In conclusion, bambara groundnut contained allergenic proteins, and the research demonstrated a cross-reactivity to peanut and soybean.

Effect of oxygenated water on immune system, malonaldehyde content and student-athlete performance.

Exercise requires more oxygen input and cannot be fully obtained through breathing. The body has a homeostatic system, when more ATP is needed, the more oxygen is required by the body. Limited supply of oxygen such as those happens in athlete’s body automatically switches the respiration from aerobic into anaerobic. This situation results in building up of lactic acid which in turn lowers the energy for cellular metabolism as well asenergy for exercise. The purpose of this study was to assess the effects of short-term and long-term drinking of oxygenated water on lactic acid and energy expenditure (EE) in exercise performance; Cluster of Differentiation-4 (CD4), CD8, CD56, Interleukin-6 (IL-6) contents; and level of malonaldehyde. During short-term intervention, 12 male student volunteers drank oxygenated (50, 80 and 130 ppm) water and normal mineral water, 15 minutes before treadmill and after treadmill (10 Kmh). The lactic acid concentration was measured before and after treadmill, and 5 minutes after the reconsumption of the oxygenated water. During long-term intervention, 17 male student volunteers drank oxygenated (100 ppm) water twice a day for 21 days. The blood plasma and lymphocyte sampled before and after intervention were analyzed for the same parameters. The results showed that oxygenated water had no effect on lactic acid accumulations and EE. Similarlyt he long-term study showed that oxygenated water did not affect CD4 and CD8, had no harmful effect on IL-6 and malonaldehyde, yet it significantly increased the CD56 content.

PENGARUH MINUMAN BEROKSIGEN TERHADAP SISTEM IMUN, KADAR MALONALDEHIDA DAN PERFORMA RESPONDEN MAHASISWA OLAHRAGAWAN [Effect of Oxygenated Water on Immune System, Malonaldehyde Content and Student-Athlete Performance]

Exercise requires more oxygen input and cannot be fully obtained through breathing. The body has a homeostatic system, when more ATP is needed, the more oxygen is required by the body. Limited supply of oxygen such as those happens in athlete’s body automatically switches the respiration from aerobic into anaerobic. This situation results in building up of lactic acid which in turn lowers the energy for cellular metabolism as well asenergy for exercise. The purpose of this study was to assess the effects of short-term and long-term drinking of oxygenated water on lactic acid and energy expenditure (EE) in exercise performance; Cluster of Differentiation-4 (CD4), CD8, CD56, Interleukin-6 (IL-6) contents; and level of malonaldehyde. During short-term intervention, 12 male student volunteers drank oxygenated (50, 80 and 130 ppm) water and normal mineral water, 15 minutes before treadmill and after treadmill (10 Kmh). The lactic acid concentration was measured before and after treadmill, and 5 minutes after the reconsumption of the oxygenated water. During long-term intervention, 17 male student volunteers drank oxygenated (100 ppm) water twice a day for 21 days. The blood plasma and lymphocyte sampled before and after intervention were analyzed for the same parameters. The results showed that oxygenated water had no effect on lactic acid accumulations and EE. Similarlyt he long-term study showed that oxygenated water did not affect CD4 and CD8, had no harmful effect on IL-6 and malonaldehyde, yet it significantly increased the CD56 content.

PENGARUH MINUMAN BEROKSIGEN TERHADAP SISTEM IMUN, KADAR MALONALDEHIDA DAN PERFORMA RESPONDEN MAHASISWA OLAHRAGAWAN

Exercise requires more oxygen input and cannot be fully obtained through breathing. The body has a homeostatic system, when more ATP is needed, the more oxygen is required by the body. Limited supply of oxygen such as those happens in athlete’s body automatically switches the respiration from aerobic into anaerobic. This situation results in building up of lactic acid which in turn lowers the energy for cellular metabolism as well asenergy for exercise. The purpose of this study was to assess the effects of short-term and long-term drinking of oxygenated water on lactic acid and energy expenditure (EE) in exercise performance; Cluster of Differentiation-4 (CD4), CD8, CD56, Interleukin-6 (IL-6) contents; and level of malonaldehyde. During short-term intervention, 12 male student volunteers drank oxygenated (50, 80 and 130 ppm) water and normal mineral water, 15 minutes before treadmill and after treadmill (10 Kmh). The lactic acid concentration was measured before and after treadmill, and 5 minutes after the reconsumption of the oxygenated water. During long-term intervention, 17 male student volunteers drank oxygenated (100 ppm) water twice a day for 21 days. The blood plasma and lymphocyte sampled before and after intervention were analyzed for the same parameters. The results showed that oxygenated water had no effect on lactic acid accumulations and EE. Similarlyt he long-term study showed that oxygenated water did not affect CD4 and CD8, had no harmful effect on IL-6 and malonaldehyde, yet it significantly increased the CD56 content.

Extraction of Squid (Photololigo Duvaucelii) Myofibrillar and Sarcoplasmic Proteins to Create A Skin Prick Test Reagent In The Diagnosis of Food Allergy

The major and minor allergens in fish and shellfish that have been identified are broadly spread in the myofibrillar and sarcoplasmic proteins. This study aimed to create the SPT reagents of squid myofibrillar and sarcoplasmic proteins to improve sensitivity and specificity diagnosis. Myofibrillar protein fraction was composed of 11 protein bands with the molecular weights of 18.2-108.4 kDa and sarcoplasmic protein fraction was composed of 13 protein bands with the molecular weights of 9.5-119.3 kDa. The allergenic myofibrillar protein has the molecular weights of 8.9-122.7 kDa while the allergenic sarcoplasmic protein has the molecular weights of 11.8-143.9 kDa. Each allergen extract was formulated into a SPT reagent met the requirements of the European Pharmacopoeia Monograph on Allergen Products 7 (2010:1063) for the parameters of moisture content, protein content, sterility and microbiology. A SPT to the respondents indicated that the sensitivity value of squid myofibrillar reagent was 86% and squid sarcoplasmic reagent was 64% with a negative error rate for each myofibrillar and sarcoplasmic reagents was 14% and 36%, respectively. The result diagnosis of squid myofibrillar and sarcoplasmic would generate sensitivity value at 100%. The separation of squid proteins into myofibrils and sarcoplasm for SPT could improve its sensitivity value and lowered the negative misdiagnosis. The specificity of each myofibrillar and sarcoplasmic proteins was 100% with an error rate of positive diagnosis occurrence at 0%.