Erhan Gökçe

Determination of cardiac troponin I in the blood and heart of calves with foot-and-mouth disease

The current study was designed to determine the changes of the cardiac troponin I (cTnI) expression in blood and tissue during the myocardial degeneration in calves with foot-and-mouth disease (FMD). Seventeen crossbred calves presenting pathological signs for FMD confirmed by viral analysis were studied. A biochemistry panel and immunohistochemistry were performed on 17 diseased calves and 7 calves used as controls. Creatine kinase (CK), CK-myocardial band (CK-MB), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) activities were analyzed for both groups. Cardiac troponin I levels were measured by a commercially available enzyme-linked immunosorbent assay kit. Mean cTnI (14.8 ± 1.9 ng/ml) concentration and CK (573 ± 407 U/l), CK-MB (238 ± 37 U/l), AST (84 ± 7), and LDH (298 ± 29 U/l) activities were higher in FMD cases compared with controls. Immunohistochemistry revealed loss or depletion of cTnI expression in myocardium of all cases. None of the 7 controls showed loss of cTnI expression. Increased serum cTnI concentration correlated with myocardial injury and loss of cTnI immunolabeling in cardiomyocytes of calves with FMD.

Application of Small Ruminant Database and Data Analysis System (Desktop and Android Application)

I n t o d a y ’ s w o r l d , v a r i o u s i n f o r m a t i o n s y st e m s h a v e b een d e s i g n ed f o r h e l p i n g t h e p h y s i c i a n s i n m e d i c i n e f i e l d . B y m ea n s o f t h e s e s y s t e m s , t h e d a t a a ss o c i a t ed w i t h t h e p a t i e n t s are s t o re d , s h ar e d e a s il y o r t h e a n a l y s i s a b o u t t h e p a t i e n t s c a n b e d o n e. I n a n i m al h ea l t h , w h i ch i s a l s o a s i m p o r t a n t a s hu m an h e a l t h , i t i s s e en t h at a n a l y s e s a b o u t t h e i l l n e ss e s a r e q u i t e l i mi t e d . I n o u r o p i n i o n , o n e o f t h e m o s t i m p o r t a n t re a s o n s f o r t h i s i s t h e l ack o f a s y st em w i t h w h i c h t h e d a t a o n t h e a n i m a l s a re st o r e d a n d s h a r e d . B e ca u s e o f t h e l ack o f t h i s k i n d o f s y st e m , l i m i t ed s t ud i es h a v e b e en d o n e o n a n i m a l i l l n e s s es a n d a l t er n a t i v e s o l u t i o n s c o u l d n o t b e p r ov i d ed i n d e t a i l f o r i l l n e ss e s a n d d ea t h s . T h u s , t h e d e c r ea s e i n a n i m a l s a n d a n i m al p r o du c t i o n s h a s b e en r i s i n g d ay b y d ay a n d t h e e c o n o m y o f t h e c o un t ry h a s b e e n a f f e c t e d n eg a t i v e l y . I n t h i s st ud y , a s y st em h as b een p r ov i d ed i n o r d e r t o st o r e , s h a r e a n d r u n d a t a a n a l y s i s r e gar d i n g t h e rec o r d s o f a n i m a l s . I n a d d i t i o n , v i s u al i n t e r f a c es h a v e b e en p r e p a r ed t o f a c i l i t a t e t h e d a t a b a s e p r o ce d u r e s .

Efficacy of pomades containing different percentages of enilconazole in the treatment of bovine dermatophytosis.

BACKGROUND Enilconazole is a broad-spectrum topical antimycotic agent used for the management of bovine dermatophytosis. HYPOTHESIS/OBJECTIVES To investigate the efficacy of pomades containing different concentrations of enilconazole for the treatment of bovine dermatophytosis. METHODS Dermatophytosis was confirmed in 120 cattle from farm in Gole region of Turkey. Animals were divided into six groups (n = 20 in each). Pomades containing 1%, 2%, 3%, 4% and 5% enilconazole were applied topically to individual lesions in groups I-V, respectively, once a day for 3 days. Group VI animals were used as a control group. Animals were monitored clinically once a week for a two month period. RESULTS Cows treated with pomades containing 4% and 5% enilconazole recovered; adverse topical reactions occurred in 40% and 55% of animals, respectively. The success rate for cows treated with pomades containing 3% enilconazole was 95% and they recovered with no adverse reactions. Success rates for treatment were 25% and 50% for cows treated with pomades containing 1% and 2% enilconazole, respectively. No improvement was observed in the control group. CONCLUSIONS AND CLINICAL IMPORTANCE Pomades containing 3% enilconazole are recommended for the treatment of bovine dermatophytosis.

Effectiveness of Enilconazole (10% pour-on) for the Treatment of Bovine Dermatophytosis

Çal şman n materyalini, Kars ve çevre köylerinde klinik ve mikrobiyolojik olarak dermatofitozis teşhisi konulan, 2.5-12 ayl k, 16 deneme ve 10 kontrol olmak üzere toplam 26 s ğ r oluşturdu. Hayvanlar n klinik durumlar ; lezyonlar n lokalizasyonu, büyüklüğü, say lar ve kondüsyonlar na göre, hafif (+), orta (++) ve şiddetli (+++) olarak skorland r ld . Çal şma süresince deneme ve kontrol grubundaki hayvanlar n bak m, besleme ve bar nak koşullar nda herhangi bir değişiklik yap lmad . Çal şma gruplar aras nda bir örneklik oluşturmak için s ğ rlar n klinik durumlar na göre; deneme grubu 4’ü hafif, 7’si orta ve 5’i şiddetli olmak üzere toplam 16 s ğ rdan oluşturuldu. Bu gruba, cidagodan sakruma kadar orta hat boyunca (3 gün ara ile 5 kez) 4 mg/kg dozda %10’luk enilconazole solüsyonu dökme şeklinde haricen uyguland . Kontrol grubu ise 3’ü hafif, 4’ü orta ve 3’ü şiddetli olmak üzere toplam 10 s ğ rdan oluşturuldu ve bu gruba sağalt m uygulanmad . Sonuç olarak, tedaviye al nan 16 hayvan n 2’si tamamen iyileşirken, 14’ünde iyileşme görülmedi. Bununla birlikte, ilac n uygulama hatt nda rastlant sal olarak bulunan dermatofitozis lezyonlar nda h zl iyileşmenin görülmesi nedeniyle, ilac n dermatofitozis olgular n n tedavisinde lokal olarak kullan m n n araşt r lmas n n gerektiği kan s na var ld .