Hidayet Metin Erdogan

Determination of cardiac troponin I in the blood and heart of calves with foot-and-mouth disease

The current study was designed to determine the changes of the cardiac troponin I (cTnI) expression in blood and tissue during the myocardial degeneration in calves with foot-and-mouth disease (FMD). Seventeen crossbred calves presenting pathological signs for FMD confirmed by viral analysis were studied. A biochemistry panel and immunohistochemistry were performed on 17 diseased calves and 7 calves used as controls. Creatine kinase (CK), CK-myocardial band (CK-MB), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) activities were analyzed for both groups. Cardiac troponin I levels were measured by a commercially available enzyme-linked immunosorbent assay kit. Mean cTnI (14.8 ± 1.9 ng/ml) concentration and CK (573 ± 407 U/l), CK-MB (238 ± 37 U/l), AST (84 ± 7), and LDH (298 ± 29 U/l) activities were higher in FMD cases compared with controls. Immunohistochemistry revealed loss or depletion of cTnI expression in myocardium of all cases. None of the 7 controls showed loss of cTnI expression. Increased serum cTnI concentration correlated with myocardial injury and loss of cTnI immunolabeling in cardiomyocytes of calves with FMD.

Molecular and serological evidence of Anaplasma phagocytophilum infection of farm animals in the Black Sea Region of Turkey

This study was designed to determine the presence and the prevalence of Anaplasma phagocytophilum infection in sheep and cattle in the Middle and Eastern Black Sea Regions of Turkey in which the potential vector, Ixodes ricinus, is widespread. Blood samples were collected from 720 sheep and 720 cattle from 6 provinces of the region, and used for detecting antibodies to A. phagocytophilum by indirect immunofluorescent antibody test (IFAT) and specific nucleic acids by a nested polymerase chain reaction (PCR). Blood smears were also prepared and examined microscopically for the presence of A. phagocytophilum-like organisms in polymorphonuclear cells. Of the animals examined, antibodies were detected in 110 (15.27%) cattle and 107 (14.86%) sheep and A. phagocytophilum-like organisms were detected in the blood of 73 (10.13%) cattle and 71 (9.86%) sheep. In addition, specific DNA was detected in the blood of 27 (14.75%) cattle and 22 (12.35%) sheep. The results obtained constitute the first molecular and serological evidence of A. phagocytophilum infection in sheep and cattle in the Black Sea Region of Turkey.

Detection of myocardial degeneration with point-of-care cardiac troponin assays and histopathology in lambs with white muscle disease.

The aim of this study was to evaluate the use of human cardiac troponin-I (cTn-I) and cardiac troponin-T (cTn-T) kits for the determination of myocardial degeneration in lambs suffering from white muscle disease (WMD). Cardiac troponin (cTn) analyses and necropsy were performed on 12 lambs with acute WMD. Only cTn analyses were tested in six healthy lambs. cTn-I and cTn-T tests were positive for all lambs with WMD, but negative in healthy lambs. Necropsy revealed that the cardiac and skeletal muscles of lambs with WMD had chalky white lesions, which appeared as necrosis and calcification in histopathology. The histopathological findings of the heart muscle and increased cTn in lambs with WMD suggested that marked myocardial degeneration may be detected by point-of-care cTn assays in lambs.

P141 The investigation of selegiline and rasagiline administration on QT interval in conscious rabbits

reflexes -(C) and negative reflexes -(-)). This testified to deficiency in vagus system reflexes, which main function is regulation of internals and provision of blood distribution in accordance with body needs. The reflexes pathology was connected with clinical neurological preeclampsia symptoms (headache, dizziness, “black flies” in front of the eyes, pain in epigastric zone). The moderate preeclampsia reflexes had the following characteristics: oculocardiac -(+)14, C1, (++)6, (-)2; orbital -(+)15, CO, (++)7, (-)1; celiac-plexus reflex-(+)19, (++)2, (-)2. Accordingly, the severe preeclampsia reflexes had the following characteristics: oculocardiac -(+)9, C4, (++)9, (-)5; orbital -(+)10, C3, (++)8, (-)6; celiac-plexus reflex-(+)11, (++)10, (-)6. Conclusions: Vegetative dystonia syndrome reflects dependence between preeclampsia severity and degree of its neurological manifestations. The increase in preeclampsia severity means poor vegetative reflexes and pronounced imbalance between sympathetic and parasympathetic nervous systems. This should be taken into account during treatment and preparation of pregnant women for delivery, because labor decompensates insufficient adaptation mechanisms through hemorrhage and loss of blood. These complications are realized with participation of nervus vagus system.