Eva María Torrecilla Sánchez

Kinetic and enantioselective behavior of the lipase from Candida cylindracea: A comparative study between the soluble enzyme and the enzyme immobilized on agarose and silica gels

Candida cylindracea lipase has previously been covalently immobilized on agarose by the tosylation method and on silica by the trichlorotiazine method. We describe a simultaneous study of the soluble enzyme and its counterpart immobilized derivatives and quantify the differences between the kinetic behavior of the three forms. The dependence of lipase activity on Na(I) and Ca(II) concentrations has been examined for the three forms of the enzyme, and results interpreted. Kinetic studies with p-nitrophenyl acetate (esterase activity) and tributyrine (lipase activity) as substrates revealed that the immobilized derivatives have lower kcatapp and Kmapp values than the soluble enzyme. With both substrates, the parallel decrease in kcatapp and Kmapp produces the favorable effect of maintaining the kcatappKmapp ratios of the supported enzymes at values comparable to those of the soluble enzyme. Kinetic studies with methyl (R)-(+) and methyl (S)-(−)-2-chloro-propionates (enantioselective activity) point to certain deviations in the Michaelis-Menten behavior. Accordingly, the v versus [S] curves were fitted by the cubic splines method and interpreted using the areas under the curves. A new type of average enantiomeric excess calculated from these areas varied from 6.4% for the soluble enzyme to 24 and 42% when immobilized on agarose and silica, respectively. This is interpreted in terms of a rigidification of the enzyme produced by the covalent immobilization.

Combining rimonabant and fentanyl in a single entity: preparation and pharmacological results

Based on numerous pharmacological studies that have revealed an interaction between cannabinoid and opioid systems at the molecular, neurochemical, and behavioral levels, a new series of hybrid molecules has been prepared by coupling the molecular features of two wellknown drugs, ie, rimonabant and fentanyl. The new compounds have been tested for their affinity and functionality regarding CB1 and CB2 cannabinoid and μ opioid receptors. In [35S]-GTPγS (guanosine 5′-O-[gamma-thio]triphosphate) binding assays from the post-mortem human frontal cortex, they proved to be CB1 cannabinoid antagonists and μ opioid antagonists. Interestingly, in vivo, the new compounds exhibited a significant dual antagonist action on the endocannabinoid and opioid systems.

Experimental evaluation of emotional development programmes for teachers in Secondary Education

espanolLa inteligencia emocional es un factor predictivo de bienestar entre los docentes. Por lo tanto, el debate se centra en la pertinencia y la necesidad de desarrollar competencias en profesores de educacion secundaria. Esto implica el diseno, implementacion y evaluacion de dos programas de capacitacion vinculados a la gestion de conflictos y la promocion de las competencias emocionales. La eficacia de los programas de capacitacion se estudia mediante la adopcion de un diseno de investigacion de tipo preexperimental con medidas previas y posteriores a la prueba. Los resultados obtenidos tras la aplicacion de ambos programas muestran cambios estadisticamente significativos (p EnglishEmotional intelligence is a predictive factor of wellbeing among teachers. The discussion therefore focuses on the pertinence of, and need for, developing competences in Secondary Education teachers. This involves the design, implementation and evaluation of two training programmes linked to conflict management and the promotion of emotional competences. The efficacy of the training programmes is studied through the adoption of a pre-experimental type research design with pre/post-test measures. The results obtained following the application of both these programmes show statistically significant changes (p

The technological model in the school guidance into digital educational resources

The depth of teaching properties of digital resources in guidance is discussed in this paper through the possibility of identifying orientation models in their theoretical structure. We study the possibility of identifying the main theoretical features underlying in each of them, and we study also their connection with some accuracy to these models. More particularly, the present study aims to assess the existence of a technological model of guidance, due to the incorporation of Information and Communication Technologies to the educational guidance processes. This technological model would be an extension of the classical models, traditionally considered.