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Dive into the research topics where Fabian Göttfert is active.

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Featured researches published by Fabian Göttfert.


Nature Methods | 2014

Fluorogenic probes for live-cell imaging of the cytoskeleton

Gražvydas Lukinavičius; Luc Reymond; Elisa D'Este; Anastasiya Masharina; Fabian Göttfert; Haisen Ta; Angelika Güther; Mathias Fournier; Stefano Rizzo; Herbert Waldmann; Claudia Blaukopf; Christoph Sommer; Daniel W. Gerlich; Hans-Dieter Arndt; Stefan W. Hell; Kai Johnsson

We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.


Biophysical Journal | 2013

Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution.

Fabian Göttfert; Christian A. Wurm; Veronika Mueller; Sebastian Berning; Volker C. Cordes; Alf Honigmann; Stefan W. Hell

We report on a fiber laser-based stimulated emission-depletion microscope providing down to ∼20 nm resolution in raw data images as well as 15-19 nm diameter probing areas in fluorescence correlation spectroscopy. Stimulated emission depletion pulses of nanosecond duration and 775 nm wavelength are used to silence two fluorophores simultaneously, ensuring offset-free colocalization analysis. The versatility of this superresolution method is exemplified by revealing the octameric arrangement of Xenopus nuclear pore complexes and by quantifying the diffusion of labeled lipid molecules in artificial and living cell membranes.


The EMBO Journal | 2014

Developmental refinement of hair cell synapses tightens the coupling of Ca2+ influx to exocytosis.

Aaron B. Wong; Mark A. Rutherford; Mantas Gabrielaitis; Tina Pangršič; Fabian Göttfert; Thomas Frank; Susann Michanski; Stefan W. Hell; Fred Wolf; Carolin Wichmann; Tobias Moser

Cochlear inner hair cells (IHCs) develop from pre‐sensory pacemaker to sound transducer. Here, we report that this involves changes in structure and function of the ribbon synapses between IHCs and spiral ganglion neurons (SGNs) around hearing onset in mice. As synapses matured they changed from holding several small presynaptic active zones (AZs) and apposed postsynaptic densities (PSDs) to one large AZ/PSD complex per SGN bouton. After the onset of hearing (i) IHCs had fewer and larger ribbons; (ii) CaV1.3 channels formed stripe‐like clusters rather than the smaller and round clusters at immature AZs; (iii) extrasynaptic CaV1.3‐channels were selectively reduced, (iv) the intrinsic Ca2+ dependence of fast exocytosis probed by Ca2+ uncaging remained unchanged but (v) the apparent Ca2+ dependence of exocytosis linearized, when assessed by progressive dihydropyridine block of Ca2+ influx. Biophysical modeling of exocytosis at mature and immature AZ topographies suggests that Ca2+ influx through an individual channel dominates the [Ca2+] driving exocytosis at each mature release site. We conclude that IHC synapses undergo major developmental refinements, resulting in tighter spatial coupling between Ca2+ influx and exocytosis.


Optical Nanoscopy | 2012

Novel red fluorophores with superior performance in STED microscopy

Christian A. Wurm; Kirill Kolmakov; Fabian Göttfert; Haisen Ta; Mariano L. Bossi; Heiko Schill; Sebastian Berning; Stefan Jakobs; Gerald Donnert; Vladimir N. Belov; Stefan W. Hell

In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion (STED) microscopy, providing < 25 nm spatial resolution in raw images of cells.


Scientific Reports | 2015

The tetraspanin web revisited by super-resolution microscopy

Malou Zuidscherwoude; Fabian Göttfert; Vera Marie E. Dunlock; Carl G. Figdor; Geert van den Bogaart; Annemiek B. van Spriel

The spatial organization of membrane proteins in the plasma membrane is critical for signal transduction, cell communication and membrane trafficking. Tetraspanins organize functional higher-order protein complexes called ‘tetraspanin-enriched microdomains (TEMs)’ via interactions with partner molecules and other tetraspanins. Still, the nanoscale organization of TEMs in native plasma membranes has not been resolved. Here, we elucidated the size, density and distribution of TEMs in the plasma membrane of human B cells and dendritic cells using dual color stimulated emission depletion (STED) microscopy. We demonstrate that tetraspanins form individual nanoclusters smaller than 120 nm and quantified that a single tetraspanin CD53 cluster contains less than ten CD53 molecules. CD53 and CD37 domains were adjacent to and displayed only minor overlap with clusters containing tetraspanins CD81 or CD82. Moreover, CD53 and CD81 were found in closer proximity to their partners MHC class II and CD19 than to other tetraspanins. Although these results indicate that tetraspanin domains are adjacently positioned in the plasma membrane, they challenge the current view of the tetraspanin web of multiple tetraspanin species organized into a single domain. This study increases the molecular understanding of TEMs at the nanoscale level which is essential for comprehending tetraspanin function in cell biology.


Journal of the American Chemical Society | 2016

Fluorogenic Probes for Multicolor Imaging in Living Cells

Gražvydas Lukinavičius; Luc Reymond; Keitaro Umezawa; Olivier Sallin; Elisa D’Este; Fabian Göttfert; Haisen Ta; Stefan W. Hell; Yasuteru Urano; Kai Johnsson

Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes, and SNAP-tag are fluorogenic, cell-permeable, and compatible with superresolution microscopy. In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding our capacity for imaging living cells.


Nature Communications | 2015

Hydrophobic mismatch sorts SNARE proteins into distinct membrane domains

Dragomir Milovanovic; Alf Honigmann; Seiichi Koike; Fabian Göttfert; Gesa Pähler; Meike Junius; Stefan Müllar; Ulf Diederichsen; Andreas Janshoff; Helmut Grubmüller; Herre Jelger Risselada; Christian Eggeling; Stefan W. Hell; Geert van den Bogaart; Reinhard Jahn

The clustering of proteins and lipids in distinct microdomains is emerging as an important principle for the spatial patterning of biological membranes. Such domain formation can be the result of hydrophobic and ionic interactions with membrane lipids as well as of specific protein–protein interactions. Here using plasma membrane-resident SNARE proteins as model, we show that hydrophobic mismatch between the length of transmembrane domains (TMDs) and the thickness of the lipid membrane suffices to induce clustering of proteins. Even when the TMDs differ in length by only a single residue, hydrophobic mismatch can segregate structurally closely homologous membrane proteins in distinct membrane domains. Domain formation is further fine-tuned by interactions with polyanionic phosphoinositides and homo and heterotypic protein interactions. Our findings demonstrate that hydrophobic mismatch contributes to the structural organization of membranes.


Scientific Reports | 2016

Subcortical cytoskeleton periodicity throughout the nervous system

Elisa D'Este; Dirk Kamin; Caroline Velte; Fabian Göttfert; Mikael Simons; Stefan W. Hell

Superresolution fluorescence microscopy recently revealed a ~190 nm periodic cytoskeleton lattice consisting of actin, spectrin, and other proteins underneath the membrane of cultured hippocampal neurons. Whether the periodic cytoskeleton lattice is a structural feature of all neurons and how it is modified when axons are ensheathed by myelin forming glial cells is not known. Here, STED nanoscopy is used to demonstrate that this structure is a commonplace of virtually all neuron types in vitro. To check how the subcortical meshwork is modified during myelination, we studied sciatic nerve fibers from adult mice. Periodicity of both actin and spectrin was uncovered at the internodes, indicating no substantial differences between unmyelinated and myelinated axons. Remarkably, the actin/spectrin pattern was also detected in glial cells such as cultured oligodendrocyte precursor cells. Altogether our work shows that the periodic subcortical cytoskeletal meshwork is a fundamental characteristic of cells in the nervous system and is not a distinctive feature of neurons, as previously thought.


Neuron | 2014

Uniquantal release through a dynamic fusion pore is a candidate mechanism of hair cell exocytosis

Nikolai M. Chapochnikov; Hideki Takago; Chao Hua Huang; Tina Pangršič; Darina Khimich; Jakob Neef; Elisabeth Auge; Fabian Göttfert; Stefan W. Hell; Carolin Wichmann; Fred Wolf; Tobias Moser

The mechanisms underlying the large amplitudes and heterogeneity of excitatory postsynaptic currents (EPSCs) at inner hair cell (IHC) ribbon synapses are unknown. Based on electrophysiology, electron and superresolution light microscopy, and modeling, we propose that uniquantal exocytosis shaped by a dynamic fusion pore is a candidate neurotransmitter release mechanism in IHCs. Modeling indicated that the extended postsynaptic AMPA receptor clusters enable large uniquantal EPSCs. Recorded multiphasic EPSCs were triggered by similar glutamate amounts as monophasic ones and were consistent with progressive vesicle emptying during pore flickering. The fraction of multiphasic EPSCs decreased in absence of Ca(2+) influx and upon application of the Ca(2+) channel modulator BayK8644. Our experiments and modeling did not support the two most popular multiquantal release interpretations of EPSC heterogeneity: (1) Ca(2+)-synchronized exocytosis of multiple vesicles and (2) compound exocytosis fueled by vesicle-to-vesicle fusion. We propose that IHC synapses efficiently use uniquantal glutamate release for achieving high information transmission rates.


Nature Neuroscience | 2016

Active zone scaffolds differentially accumulate Unc13 isoforms to tune Ca2+ channel-vesicle coupling

Mathias A. Böhme; Christina Beis; Suneel Reddy-Alla; Eric Reynolds; Malou M. Mampell; Andreas T. Grasskamp; Janine Lützkendorf; Dominique Dufour Bergeron; Jan H. Driller; Husam Babikir; Fabian Göttfert; Iain M. Robinson; Cahir J. O'Kane; Stefan W. Hell; Markus C. Wahl; Ulrich Stelzl; Bernhard Loll; Alexander M. Walter; Stephan J. Sigrist

Brain function relies on fast and precisely timed synaptic vesicle (SV) release at active zones (AZs). Efficacy of SV release depends on distance from SV to Ca2+ channel, but molecular mechanisms controlling this are unknown. Here we found that distances can be defined by targeting two unc-13 (Unc13) isoforms to presynaptic AZ subdomains. Super-resolution and intravital imaging of developing Drosophila melanogaster glutamatergic synapses revealed that the Unc13B isoform was recruited to nascent AZs by the scaffolding proteins Syd-1 and Liprin-α, and Unc13A was positioned by Bruchpilot and Rim-binding protein complexes at maturing AZs. Unc13B localized 120 nm away from Ca2+ channels, whereas Unc13A localized only 70 nm away and was responsible for docking SVs at this distance. Unc13Anull mutants suffered from inefficient, delayed and EGTA-supersensitive release. Mathematical modeling suggested that synapses normally operate via two independent release pathways differentially positioned by either isoform. We identified isoform-specific Unc13-AZ scaffold interactions regulating SV-Ca2+-channel topology whose developmental tightening optimizes synaptic transmission.

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Tobias Moser

University of Göttingen

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Aaron B. Wong

University of Göttingen

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Husam Babikir

Free University of Berlin

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