Fabiana Wurster

Retrospective canine skin peripheral nerve sheath tumors data with emphasis on histologic, immunohistochemical and prognostic factors

Neste estudo retrospectivo foi determinada a frequencia dos tumores de bainha de nervo periferico (TBNP) na pele de caes diagnosticados pelo Setor de Patologia Veterinaria da Universidade Federal do Rio Grande do Sul (SPV-UFRGS), Brasil, no periodo de 2000 a 2012. O historico dos caes, assim como a histologia, a imuno-histoquimica e o prognostico em relacao aos tumores foram baseados em 70 amostras histologicas, correspondendo a 40 femeas (57%), 29 machos (41%) e uma amostra sem a informacao do sexo. De 2000 a 2012, foram diagnosticados 2984 tumores de pele de caes pelo SPV-UFRGS, totalizando 2,34% das neoplasias em caes. A maior parte das amostras (43%) foi proveniente de animais sem raca definida (SRD), seguidos pelos da raca Pastor Alemao (10%). A localizacao predominante dos TBNP desta pesquisa ocorreu em membros (40% em membro toracico e 29% em membro pelvico), afetando principalmente caes de oito a 11 anos idade (54%). As amostras foram processadas rotineiramente para coloracao de Hematoxilina e eosina, e foram realizadas as coloracoes de azul de toluidina e tricromio de Masson, assim como realizada imuno-histoquimica (IHQ) anti-vimentina, -S-100, -GFAP, -actina, von Willbrand e neurofilamento. Os fatores anisocitose e anisocariose, indice mitotico, necrose intratumoral, invasao tumoral em tecidos adjacentes, localizacao tumoral, local de recorrencia e metastase foram relacionados com o diagnostico de benignidade (49/70) ou malignidade (21/70). O padrao histologico de Antoni A foi observado com mais frequencia em tumores benignos. A IHQ auxiliou no diagnostico dos TBNP, com maiores indices de imunomarcacao anti-vimentina e S-100. A analise estatistica das amostras de animais que apresentaram recidiva tumoral evidenciou que a chance de um animal com tumor de bainha de nervo periferico maligno desenvolver recidiva e 4,61 vezes maior do que em animais que apresentam tumor benigno.

Equine salmonellosis in southern Brazil

The Salmonella sp. genus is identified in several species, and the zoonosis it causes is one of the most important types worldwide. The specifics of salmonellosis vary according to the function of the serovar involved, the species affected, age and predisposing factors. However, few cases of equine salmonellosis have been reported. This study presents ten confirmed salmonellosis cases in equines in southern Brazil. Six were adult animals with stress factors preceding the disease, while four were foals, three of which presented with hyperacute manifestations. The main clinical signs were diarrhea, anorexia, and hyperthermia. Lesions varied in distribution and severity, although fibrinonecrotic or necrohemorrhagic enteritis was observed in all animals, mainly in the large intestine (large colon and cecum—8/10) and small intestine (3/10). Substantial liquid content, mainly hemorrhagic, was observed in all animals. The most characteristic microscopic lesion was mucosa necrosis, which is often accompanied by fibrin deposition, followed by necrosis of follicular centers and vascular changes. Bacterial isolation revealed seven isolates. Five were serotyped, and the serovars Typhimurium and Anatum were associated with two cases each, while Muenster was associated with a case whose lesion pattern varied. Immunohistochemical staining was positive in all cases. All diagnoses were based on the clinical history, macroscopic and histological lesions, and the bacterial isolation and/or immunostaining associated with histological lesions.

Intoxicação aguda por cobre em ovinos

Cases of acute copper intoxication in Santa Ines sheep are described. The disease occurred on a farm in Rio Grande do Sul. Three ewes that had recently lambed and that were fed cattle diet became severely ill and died one week after ingestion of a commercial mineral mix for beef cattle. The sheep showed anorexia, lateral and sternal recumbency, fasciculation and muscle stiffness and death. At necropsy from one of these sheep, the main changes were moderate hepatomegaly, prominent hepatic lobular pattern, and multifocal randomly distributed erosions and ulcerations on the abomasal mucosa. Histological evaluation of the liver showed marked centrilobular hepatocellular necrosis; diffuse hepatocyte swelling predominantly in the periportal area, and a few Kupffer cells contained cytoplasmic pigment granules. The diagnosis was based on the clinical history, necropsy and histopathologic findings, and confirmed by the high copper concentrations detected in the liver, feed, and mineral mix samples.