Gabriel Klein

Is Sterile Better Than Aseptic? Comparing the Microbiology of Acellular Dermal Matrices.

Introduction: Postoperative infections are a major complication associated with tissue-expander-based breast reconstruction. The use of acellular dermal matrix (ADM) in this surgery has been identified as a potential reservoir of infection, prompting the development of sterile ADM. Although aseptic and sterile ADMs have been investigated, no study has focused on the occurrence and clinical outcome of bacterial colonization before implantation. Methods: Samples of aseptic AlloDerm, sterile Ready-To-Use AlloDerm, and AlloMax were taken before implantation. These samples were incubated in Tryptic soy broth overnight before being streaked on Trypticase soy agar, MacConkey agar, and 5% blood agar plates for culture and incubated for 48 hours. Culture results were cross-referenced with patient outcomes for 1 year postoperatively. Results: A total of 92 samples of ADM were collected from 63 patients. There were 15 cases of postoperative surgical site infection (16.3%). Only 1 sample of ADM (AlloMax) showed growth of Escherichia coli, which was likely a result of contamination. That patient did not develop any infectious sequelae. Patient outcomes showed no difference in the incidence of seroma or infection between sterile and aseptic ADMs. Conclusions: This study evaluates the microbiology of acellular dermal matrices before use in breast reconstruction. No difference was found in the preoperative bacterial load of either aseptic or sterile ADM. No significant difference was noted in infection or seroma formation. Given these results, we believe aseptic processing used on ADMs is equivalent to sterile processing in our patient cohort in terms of clinical infection and seroma occurrence postoperatively.

Abstract: 14.20 Comparison Of The Basal View And A Previously Standardized Cleft Lip Rating Scale

IntroductIon: Complete clefts lip and palate represent a long journey for patients, families and the cleft team. We have to face a lack of function, symmetry and shape. There are involved soft tissue, muscles, cartilages, bone and all of them are displaced, distorted or missed. In more than 40 years of experience in our department we developed a strong surgical protocol that has a its center the cheilo-rhyno-plasty and periosteoplasty procedure. Within 3 hours procedure we try to restore every single part involved in order to achieve better results and less need of further procedures.

Abstract: 10.00 Development Of A Novel Way To Create Larger Vascular Conduits Of Different Biomaterials

INTrOdUCTION: Methicillin-resistant Staphylococcus aureus is one of the most common microorganisms isolated in graft lysis and septic patients with extensive burns. The main objective of this study is to determine and quantitatively measure the protective and/or therapeutic effect of Lactobacillus plantarum application on the burn wound before and after the onset of Methicillinresistant Staphylococcus aureus infection.

Bariatric surgery for obesity

One of the current burdens placing significant strain on the health care system in the United States…