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Featured researches published by Geeta Kapadia.


Journal of Molecular Biology | 1990

Crystallization of the Bacillus subtilis histidine-containing phosphocarrier protein HPr and of some of its site-directed mutants

Geeta Kapadia; Jonathan Reizer; S L Sutrina; Milton H. Saier; Prasad T. Reddy; Osnat Herzberg

The histidine-containing phosphocarrier protein (HPr) from Bacillus subtilis has been crystallized. Two of the site-directed mutants aimed at probing function produce crystals suitable for X-ray studies. The mutant in which His15 is substituted by an alanyl residue crystallizes from ammonium sulfate solution in space group P3(1)21 or P3(2)21, with unit cell dimensions: a = b = 47.3 A; c = 61.5 A. These crystals diffract to at least 1.8 A resolution. The mutant in which Ser46 is substituted by an aspartyl residue crystallizes from polyethylene glycol 4000 solution in space group P2(1), with unit cell dimensions: a = 49.4 A; b = 25.6 A; c = 60.3 A; beta = 109 degrees. These crystals diffract to at least 2.0 A resolution.


Journal of Molecular Biology | 1991

Crystallization of the IIA domain of the glucose permease of Bacillus subtilis.

Geeta Kapadia; Celia C. H. Chen; Prasad T. Reddy; Milton H. Saier; Jonathan Reizer; Osnat Herzberg

The IIA domain of the glucose permease of the phosphoenolpyruvate: sugar phosphotransferase system (PTS) from Bacillus subtilis has been crystallized. Crystals are obtained from ammonium sulfate solution. They diffract to at least 2.2 A resolution, and belong to space group C222(1), with unit cell dimensions: a = 74.2 A; b = 54.9 A; c = 67.0 A.


Proceedings of the National Academy of Sciences of the United States of America | 1996

Swiveling-domain mechanism for enzymatic phosphotransfer between remote reaction sites

Osnat Herzberg; Celia C. H. Chen; Geeta Kapadia; Marielena McGuire; Lawrence J. Carroll; Seong J. Noh; Debra Dunaway-Mariano


Proceedings of the National Academy of Sciences of the United States of America | 1992

Structure of the histidine-containing phosphocarrier protein HPr from Bacillus subtilis at 2.0-A resolution.

Osnat Herzberg; Prasad T. Reddy; S L Sutrina; Milton H. Saier; Jonathan Reizer; Geeta Kapadia


Biochemistry | 1991

Structure of the IIA domain of the glucose permease of Bacillus subtilis at 2.2-A resolution.

Der Ing Liao; Geeta Kapadia; Prasad T. Reddy; Milton H. Saier; Jonathan Reizer; Osnat Herzberg


Biochemistry | 1998

ROLE OF THE OMEGA -LOOP IN THE ACTIVITY, SUBSTRATE SPECIFICITY, AND STRUCTURE OF CLASS A BETA -LACTAMASE

Soojay Banerjee; Ursula Pieper; Geeta Kapadia; Lewis K. Pannell; Osnat Herzberg


Biochemistry | 1991

Structural basis for the inactivation of the P54 mutant of beta-lactamase from Staphylococcus aureus PC1.

Osnat Herzberg; Geeta Kapadia; Bernardo Blanco; Tom S. Smith; Andrew F. W. Coulson


Biochemistry | 1996

Elimination of the hydrolytic water molecule in a class A beta-lactamase mutant: crystal structure and kinetics.

Laura E. Zawadzke; Celia C. H. Chen; Soojay Banerjee; Zhong Li; Susana Wäsch; Geeta Kapadia; John Moult; Osnat Herzberg


Biochemistry | 1996

Structure and kinetics of the beta-lactamase mutants S70A and K73H from Staphylococcus aureus PC1.

Celia C. Chen; Thomas J. Smith; Geeta Kapadia; Susana Wäsch; Laura E. Zawadzke; Andrew Coulson; Osnat Herzberg


Biochemistry | 1998

Location of the phosphate binding site within Clostridium symbiosum pyruvate phosphate dikinase.

Marielena McGuire; Kui Huang; Geeta Kapadia; Osnat Herzberg; Debra Dunaway-Mariano

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Prasad T. Reddy

National Institute of Standards and Technology

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Laura E. Zawadzke

University of Maryland Biotechnology Institute

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Marielena McGuire

University of Maryland Biotechnology Institute

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S L Sutrina

University of California

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Soojay Banerjee

Donald Danforth Plant Science Center

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Kui Huang

University of Maryland Biotechnology Institute

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