Gloria Lee
Lawrence Berkeley National Laboratory
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Publication
Featured researches published by Gloria Lee.
Journal of Clinical Investigation | 1999
Zheng-Tao Shi; Veena Afzal; Barry S. Coller; Dipti Patel; Joel Anne Chasis; Marilyn Parra; Gloria Lee; Chris Paszty; Mary E. Stevens; Loren D. Walensky; Luanne L. Peters; Narla Mohandas; Edward M. Rubin; John G. Conboy
A diverse family of protein 4.1R isoforms is encoded by a complex gene on human chromosome 1. Although the prototypical 80-kDa 4.1R in mature erythrocytes is a key component of the erythroid membrane skeleton that regulates erythrocyte morphology and mechanical stability, little is known about 4.1R function in nucleated cells. Using gene knockout technology, we have generated mice with complete deficiency of all 4.1R protein isoforms. These 4.1R-null mice were viable, with moderate hemolytic anemia but no gross abnormalities. Erythrocytes from these mice exhibited abnormal morphology, lowered membrane stability, and reduced expression of other skeletal proteins including spectrin and ankyrin, suggesting that loss of 4. 1R compromises membrane skeleton assembly in erythroid progenitors. Platelet morphology and function were essentially normal, indicating that 4.1R deficiency may have less impact on other hematopoietic lineages. Nonerythroid 4.1R expression patterns, viewed using histochemical staining for lacZ reporter activity incorporated into the targeted gene, revealed focal expression in specific neurons in the brain and in select cells of other major organs, challenging the view that 4.1R expression is widespread among nonerythroid cells. The 4.1R knockout mice represent a valuable animal model for exploring 4.1R function in nonerythroid cells and for determining pathophysiological sequelae to 4.1R deficiency.
Chromosome Research | 2009
Evgenya Y. Popova; Sharon Wald Krauss; Sarah A. Short; Gloria Lee; Jonathan Villalobos; Joan Etzell; Mark J. Koury; Paul A. Ney; Joel Anne Chasis; Sergei A. Grigoryev
Terminal erythroid differentiation in vertebrates is characterized by progressive heterochromatin formation and chromatin condensation and, in mammals, culminates in nuclear extrusion. To date, although mechanisms regulating avian erythroid chromatin condensation have been identified, little is known regarding this process during mammalian erythropoiesis. To elucidate the molecular basis for mammalian erythroblast chromatin condensation, we used Friend virus-infected murine spleen erythroblasts that undergo terminal differentiation in vitro. Chromatin isolated from early and late-stage erythroblasts had similar levels of linker and core histones, only a slight difference in nucleosome repeats, and no significant accumulation of known developmentally regulated architectural chromatin proteins. However, histone H3(K9) dimethylation markedly increased while histone H4(K12) acetylation dramatically decreased and became segregated from the histone methylation as chromatin condensed. One histone deacetylase, HDAC5, was significantly upregulated during the terminal stages of Friend virus-infected erythroblast differentiation. Treatment with histone deacetylase inhibitor, trichostatin A, blocked both chromatin condensation and nuclear extrusion. Based on our data, we propose a model for a unique mechanism in which extensive histone deacetylation at pericentromeric heterochromatin mediates heterochromatin condensation in vertebrate erythroblasts that would otherwise be mediated by developmentally-regulated architectural proteins in nucleated blood cells.
Journal of Biological Chemistry | 2000
Marilyn Parra; Philippe Gascard; Loren D. Walensky; J. Aura Gimm; Seth Blackshaw; Nadine Chan; Yuichi Takakuwa; Trish Berger; Gloria Lee; Joel Anne Chasis; Solomon H. Snyder; Narla Mohandas; John G. Conboy
Blood | 2001
Stephen F. Parsons; Gloria Lee; Frances A. Spring; Thiebaut-Noel Willig; Luanne L. Peters; J. Aura Gimm; Michael J. A. Tanner; Narla Mohandas; David J. Anstee; Joel Anne Chasis
Blood | 2006
Gloria Lee; Annie Lo; Sarah A. Short; Tosti J. Mankelow; Frances A. Spring; Stephen F. Parsons; Karina Yazdanbakhsh; Narla Mohandas; David J. Anstee; Joel Anne Chasis
Blood | 1998
Philippe Gascard; Gloria Lee; L. Coulombel; I. Auffray; M. Lum; Marilyn Parra; John G. Conboy; Narla Mohandas; Joel Anne Chasis
Journal of Biological Chemistry | 1997
P. Olivier Schischmanoff; Paul Yaswen; Marilyn Parra; Gloria Lee; Joel Anne Chasis; Narla Mohandas; John G. Conboy
Blood | 2003
Gloria Lee; Frances A. Spring; Stephen F. Parsons; Tosti J. Mankelow; Luanne L. Peters; Mark J. Koury; Narla Mohandas; David J. Anstee; Joel Anne Chasis
Molecular Biology of the Cell | 1999
Philippe Gascard; Wataru Nunomura; Gloria Lee; Loren D. Walensky; Sharon Wald Krauss; Yuichi Takakuwa; Joel Anne Chasis; Narla Mohandas; John G. Conboy
Journal of Biological Chemistry | 2002
Sharon Wald Krauss; Rebecca Heald; Gloria Lee; Wataru Nunomura; J. Aura Gimm; Narla Mohandas; Joel Anne Chasis