Graeme Gillespie

Overview of the conservation status of Australian frogs

A review of the current conservation status of Australian amphibians was recently completed as part of a World Conservation Union (IUCN) sponsored Global Amphibian Assessment (GAA). Fifty of 216 amphibian species (23%) in Australia are now recognized as threatened or extinct in accord with IUCN Red List Categories and Criteria. Here we report on the categories and criteria under which individual species qualified for listing and provide a summary of supporting information pertaining to population and distribution declines. Major threatening processes contributing to listing of species are also reviewed.

Distribution, habitat and conservation status of the Green and Golden Bell Frog Litoria aurea (Lesson 1829) (Anura: Hylidae) in Victoria

An overview of the distribution of Litoria aurea in Victoria is presented. Habitats in which the species has been recorded are described and its current conservation status is assessed. Litoria aurea is restricted to east Gippsland where it occurs predominantly at low altitudes along the coast and hinterland. The species is usually associated with stationary water bodies, mostly permanent, in both forested and cleared habitats. The species has also been found in a range of terrestrial habitats, often considerable distances from waterbodies; suggesting a high dispersive ability and importance of terrestrial habitats. Little evidence of a decline in the distribution or abundance of L. aurea was found based on comparisons of historical and recent information. Much of the habitat and range of the species was found to be secure within the region. Possible reasons for the apparent differences in population trends between New South Wales and Victoria are discussed.

The distribution, abundance and conservation status of riverine frogs in Kosciuszko National Park

A survey was conducted of the distribution and abundance of stream-breeding frogs within Kosciuszko National Park, with particular emphasis upon locating new populations of the endangered Spotted Tree Frog Litoria spenceri. Twenty-five streams were sampled within the Park. Populations of L. spenceri ware located on two streams, including one new locality for the species. This constiiutes only the second population of this species known from New South Wales. Three other riverine species ware located during the survey. The endangered Booroolong Frog L. boorooiongensis was located only along one stream and could not be found at the two historic sites within the Park. The overall abundance of riverine frogs was found to be relatively low. Frogs were found at significantly fewer sites affected by modified stream flows from the Snowy Mountains Scheme, than comparatively undisturbed sites. The potential impacts of these changes and introduced fish upon the distribution and abundance of riverine frogs within the ...

Factors influencing occurrence of a freshwater turtle in an urban landscape: a resilient species?

Abstract Context. Species vary broadly in their ability to adapt to urbanisation. Freshwater turtles are vulnerable to the loss and degradation of terrestrial and aquatic habitat in urban environments. There have been few publications investigating impacts of urbanisation on freshwater turtles in Australia. Aims. We investigated the effects of urbanisation on the distribution and abundance of the eastern long-necked turtle (Chelodina longicollis) in greater Melbourne. Methods. We examined occurrence and relative abundance of C. longicollis at 55 wetlands across an urban–rural gradient in relation to site- and landscape-level factors. Occupancy was modelled using the program PRESENCE, and incorporated landscape and habitat covariates. A negative binomial regression model was used to examine the influence of landscape and habitat factors on relative abundance by using WinBUGS. Key results. C. longicollis occupied 85% of the 55 wetlands we surveyed, and we found no evidence that wetland occupancy was influenced by the variables we measured. However, relative abundance was highest at wetlands with low water conductivity and heavy metal pollution, and in wetlands furthest from rivers. Conclusions. C. longicollis appears to be resilient to urbanisation and is likely to persist in urban landscapes, possibly because of the creation of new wetlands in Australian cities. However, long-term studies focussed on demographic parameters, or survivorship, may elucidate as yet undetected effects of urbanisation. Although no specific management recommendations may be necessary for C. longicollis in urban areas at this time, this species may be in decline in non-urban areas as a result of climatic changes and wetland drying. Implications. Our findings suggest that caution is required before drawing generalised conclusions on the impacts of urbanisation on turtles, as the effects are likely to be species-specific, dependent on specific ecology and life-history requirements. Further studies are required to ascertain these relationships for a wider array of species and over longer time spans.

Multiple cameras required to reliably detect feral cats in northern Australian tropical savanna: an evaluation of sampling design when using camera traps.

Abstract Context. Feral cats are a major cause of mammal declines and extinctions in Australia. However, cats are elusive and obtaining reliable ecological data is challenging. Although camera traps are increasingly being used to study feral cats, their successful use in northern Australia has been limited. Aims. We evaluated the efficacy of camera-trap sampling designs for detecting cats in the tropical savanna of northern Australia. We aimed to develop a camera-trapping method that would yield detection probabilities adequate for precise occupancy estimates. Methods. First, we assessed the influence of two micro-habitat placements and three lure types on camera-trap detection rates of feral cats. Second, using multiple camera traps at each site, we examined the relationship between sampling effort and detection probability by using a multi-method occupancy model. Key results. We found no significant difference in detection rates of feral cats using a variety of lures and micro-habitat placement. The mean probability of detecting a cat on one camera during one week of sampling was very low (p = 0.15) and had high uncertainty. However, the probability of detecting a cat on at least one of five cameras deployed concurrently on a site was 48% higher (p = 0.22) and had a greater precision. Conclusions. The sampling effort required to achieve detection rates adequate to infer occupancy of feral cats by camera trap is considerably higher in northern Australia than has been observed elsewhere in Australia. Adequate detection of feral cats in the tropical savanna of northern Australia will necessitate inclusion of more camera traps and a longer survey duration. Implications. Sampling designs using camera traps need to be rigorously trialled and assessed to optimise detection of the target species for different Australian biomes. A standard approach is suggested for detecting feral cats in northern Australian savannas.

Plant selection and avoidance by the Bornean elephant (Elephas maximus borneensis) in tropical forest: does plant recovery rate after herbivory influence food choices?

The plant vigour hypothesis proposes that herbivores should favour feeding on more vigorously growing plants or plant modules. Similarly, we would expect herbivores to favour plants that regrow vigorously after herbivory. Larger animals, like elephants, may also select plant species relative to their availability and prefer species with larger growth forms in order to meet their intake requirements. The food preferences of the Bornean elephant (Elephas maximus borneensis) in the Lower Kinabatangan Wildlife Sanctuary, Sabah, Malaysia, were investigated along 12 transects in areas where elephants were recently sighted feeding. One hundred and eighty-two plants were eaten and 185 plants were measured for species availability along transects. Species vigour was determined by the monthly regrowth in new shoot length after elephant feeding and the number of new shoots produced on each plant. Measurements were carried out on each plant for 9 mo or until the new shoot was eaten. Plant sizes were determined from their basal diameter. The Bornean elephant did not prefer more vigorous species or species with larger growth forms. New shoots did not grow longer on preferred than avoided species. Additionally, unlike other elephants that live in a forest environment, the Bornean elephant preferred species from the Poaceae (specifically Phragmites karka and Dinochloa scabrida) over other plant types including gingers, palms, lianas and woody trees.

Assortative mating among animals of captive and wild origin following experimental conservation releases

Captive breeding is a high profile management tool used for conserving threatened species. However, the inevitable consequence of generations in captivity is broad scale and often-rapid phenotypic divergence between captive and wild individuals, through environmental differences and genetic processes. Although poorly understood, mate choice preference is one of the changes that may occur in captivity that could have important implications for the reintroduction success of captive-bred animals. We bred wild-caught house mice for three generations to examine mating patterns and reproductive outcomes when these animals were simultaneously released into multiple outdoor enclosures with wild conspecifics. At release, there were significant differences in phenotypic (e.g. body mass) and genetic measures (e.g. Gst and F) between captive-bred and wild adult mice. Furthermore, 83% of offspring produced post-release were of same source parentage, inferring pronounced assortative mating. Our findings suggest that captive breeding may affect mating preferences, with potentially adverse implications for the success of threatened species reintroduction programmes.

Population age structure of the spotted tree frog (Litoria spenceri): insights into population declines

Context. Despite increased scientific attention on amphibian conservation in recent years, knowledge of population demography of amphibians remains scarce, hampering evaluation of population declines and development of appropriate management responses. Aims. The aims of this research were to examine variation in population demography of the spotted tree frog (Litoria spenceri), a critically endangered species in Australia, and to evaluate the role of various factors potentially responsible for population declines such as introduced trout, chytridiomycosis and habitat changes. Methods. Skeletochronology combined with mark–recapture sampling were undertaken in two different river systems, Bogong Creek and Taponga River, to determine population age structure. Age-specific survival estimates were derived from each population and were then used to examine variance in age-specific mortality. Key results. Relative population density per 200 m of stream was 67.7 adults and 131.3 juveniles at Bogong Creek and 10.7 adults and 33.8 juveniles at Taponga River. Ages were determined for 578 frogs across the two populations. Age-specific survival was lowest in the first year of life compared to all other age classes, and highest in sexually mature adults. Differences in age-specific survival were similar between the populations, with the exception of first-year survivorship, which averaged 1.9% at Bogong Creek and 0.4% at Taponga River. This difference was large enough to explain most of the marked difference in population density between the two streams. Key conclusions. The difference in first-year age-specific survival is consistent with trout predation as the most parsimonious explanation for the large differences in population density between the populations, and lends further weight to the role of introduced trout in the decline of this species. Implications. This study has contributed to informing management actions for conservation of this species, and demonstrates that population age structure data may provide valuable insights into demographic variability within and between populations and species. This may have important implications for interpretation of population declines, and conservation and management responses.

Factors influencing detection probabilities of frogs in the monsoonal tropics of northern Australia: implications for the design of monitoring studies

Abstract Context. Failure to acknowledge potential bias from imperfect detection of cryptic organisms such as frogs may compromise survey and monitoring programmes targeting these species. Aims. The aims of the present study were to identify proximate factors influencing detection probabilities of a range of frog species in monsoonal northern Australia, and to estimate the number of repeat censuses required at a site to have confidence that non-detected species are absent. Methods. Data on detection or non-detection of frog species based on calling individuals were recorded during 10 wet-season censuses of 29 survey sites in the Darwin region. Factors influencing detection probabilities were identified using occupancy models; model selection was based on the Akaike information criterion. Sampling effort for individual species was calculated using model predictions at different stages of the wet season. Key results. The covariate water temperature featured in the best-supported models for 7 of the 14 frog species. Six of these species were more likely to be detected when water temperatures were below 30°C. Detection probabilities were also correlated with the number of days since the commencement of the wet season, time since last significant rainfall, air temperature and time after sunset. Required sampling effort for individual species varied throughout the wet season. For example, a minimum of two repeat censuses was required for detection of Litoria caerulea in the early wet season, but this number increased to 13 in the middle stage of the wet season. Conclusions. Variability in environmental conditions throughout the wet season leads to variability in detection probabilities of frog species in northern Australia. Lower water temperatures, mediated by rainfall immediately before or during surveys, enhances detectability of a range of species. For most species, three repeat surveys under conditions resulting in a high detection probability are sufficient to determine presence at a site. Implications. Survey and monitoring programmes for frogs in tropical northern Australia will benefit from the results of the present study by allowing targeting of conditions of high detection probability for individual species, and by incorporating sufficient repeat censuses to provide accurate assessment of the status of individual species at a site.

What do predator diets tell us about mammal declines in Kakadu National Park

Abstract Context. Small- and medium-sized native mammals have suffered severe declines in much of northern Australia, including within protected areas such as Kakadu National Park. Several factors have been implicated in these declines but predation, particularly by feral cats (Felis catus), has been identified as potentially the most direct cause of decline for many species. Aims. We evaluated how prey frequency changed in cat and dingo scats in Kakadu from the early 1980s to 2013–15, with this period spanning a severe decline in the small- and medium-sized mammal fauna. Methods. Chi-square test of independence and Fisher’s exact test were used to compare prey frequencies between dingoes and cats, and among years to assess significance of temporal change. Key results. Small-sized native mammals were the prey item occurring at the highest frequency in scats for both dingoes and cats in the 1980s. Prey content in dingo and cat scats differed in the 2010s with macropods predominating in the scats of dingoes, and medium-sized native mammals predominating in cat scats. The frequency of occurrence of small-sized native mammals declined in both dingo and cat scats between the 1980s and 2010 sampling periods, while the frequency of occurrence of medium-sized native mammals remained constant in dingo scats and increased in cat scats. Conclusions. Small mammals were a major component of the diets of both dingoes and cats in Kakadu in the 1980s, when small mammals were much more abundant. Despite marked reduction from the 1980s to the 2010s in the capture rates of both small- and medium-sized native mammals, some species continue to persist in the diets of cats and dingoes at disproportionally high frequencies. Both predators continue to exert predatory pressure on mammal populations that have already experienced substantial declines. Implications. Although predation by feral cats is a major threat to small- and medium-sized native mammals, dingoes may also play an important role in limiting their recovery. Disturbance from fire and grazing by introduced herbivores has been shown to augment predatory impacts of feral cats on native mammals. Predation more generally, not just by feral cats, may be exacerbated by these disturbance processes. Management programs that solely focus on mitigating the impact of feral cats to benefit threatened species may be inadequate in landscapes with other significant disturbance regimes and populations of predators.