Gyula Pinke

Effects of environmental factors on weed species composition of cereal and stubble fields in western Hungary

Multivariate analysis of data obtained from 184 cereal and stubble fields in low-input agricultural systems located in western Hungary was undertaken in order to asses environmental factors affecting weed species composition. For each variable, the gross and net effect on weed species composition was calculated. All variables considered in this study had a significant effect on weed species composition and explained 26.99% of the total variation. Most variation in species composition was explained by the aspect (cereal vs. stubble), followed by soil pH, mean annual precipitation, soil texture, mean annual temperature, and altitude. Separating the cereals and stubbles soil pH became the most important factor. Our results suggest that during the long vegetation period, cereal weed communities dominated by winter annuals are replaced by stubble-field weed communities dominated by summer annuals. This seasonal change may also have the same important effect on weed species composition as crop types.

Phytosociological and conservational study of the arable weed communities in western Hungary

Abstract The present study surveyed the weed vegetation on extensively managed arable fields and unsprayed field edges in western Hungary, based on 1698 phytosociological relevés collected between 1995 and 2005. The separation of the 15 vegetation units was conducted with the traditional comparative tabular method, and the diagnostic species were determined with statistical fidelity measures. The numerical analyses show that the most important factor in the separation of weed communities is the fluctuating proportions of winter and summer annuals and cosmopolitan elements throughout the year. Soil chemical properties are the second most important whereas soil texture and climatic factors also contribute to the development of weed communities. The surveyed vegetation units support 41 red list weed species. Across all associations, the average proportion of insect-pollinated plant species is 68%, and the average proportion providing weed seed food sources for farmland birds is 47%. These factors illustrate the importance of weed communities in agro-ecosystem function and the need for their conservation.

Assessment of endangered synanthropic plants of Hungary with special attention to arable weeds

Abstract In the present study, species were selected from the new Red List of the vascular flora of Hungary which can be regarded as a weed. For each species, current conservation status and the most important traits were assessed. Altogether 149 weed species were found to be at risk according to the International Union for Conservation of Nature (IUCN) categories: 11 species are extinct, 11 are critically endangered, 27 are endangered, 26 are vulnerable, 62 are near threatened and 12 are data deficient. These species belong to 37 plant families, from which the most important are Caryophyllaceae, Brassicaceae, Asteraceae, Scrophulariaceae, Chenopodiaceae, Poaceae and Fabaceae. The most significant chorological elements are Mediterranean (28%) and Eurasian (27%); endemic (Pannonian) taxa constitute only 5.4%. Over 90% of these species are of native or archaeophyte origin, according to their residence time. Considering the main habitat types, 46% of the species are originated from dry habitats, 23% from arable lands, 17.5% from wet habitats and 13.5% from ruderal habitats. In the life form spectra, a pronounced dominance of therophytes (81%) is represented. The factor that currently offers the greatest conflict to the conservation of endangered weed species in Hungary are side effects of strong eradication campaigns against the invasive Ambrosia artemisiifolia.

ACCase inhibitor herbicides – selectivity, weed resistance and fitness cost: a review

The worldwide application of the group of herbicides known as ACCase inhibitors is associated with the widespread appearance of resistant biotypes among weeds. Accordingly, ACCase inhibitor resistance has become the third most frequent type of weed resistance. ACCase (acetyl-coenzyme A carboxylase) inhibitors, which include aryloxyphenoxypropionate, cyclohexanedione and phenylpyrazolin herbicides, are applied postemergence to control weedy grasses. Their phytotoxicity is specifically attributed to the inhibition of lipid biosynthesis, while their selectivity is mainly due to the fact that they are able to block the eukaryote-type ACCase enzyme of Poaceae. Certain herbicides can be applied to cereals, because herbicide “safeners” allow the herbicides to be degraded metabolically. Resistance can evolve through both non-target and target-site processes, and many amino acid substitutions have been identified that result in resistance. Cross-resistance to different herbicides is also relatively common. Nevertheless, in many cases, it has been found that resistant biotypes show no reduction in fitness. Cycloxydim-tolerant maize, without genetic modification, is now used also in European farmland, and it can be a suitable alternative to transgenic herbicide tolerant crops when combined with herbicide rotation and monitoring for resistance.

Floristic composition and conservation value of the stubble-field weed community, dominated by Stachys annua in western Hungary

The stubble-field weed community, dominated by Stachys annua, was generally distributed in Hungary until the 1950s on mid-heavy and heavy, base-rich soils. Stachys annua is an excellent nectar-producer, and from the nectar collected in its habitats popular stubble-honey was produced. This vegetation type has suffered significant decline, mainly due to the early ploughing of stubbles associated with the intensification of agriculture. In the present study, the floristic composition of this community is assessed based on 213 phytosociological records, and its distribution in the past ten years in western Hungary is mapped. Sixty-five percent of the species are of Eurasian, European and Mediterranean elements, and the largest proportion of the species are spring-germinating summer annuals. The proportion of insect-pollinated plant species is approximately 70%, and the species composition also offers significant seed food sources for farmland birds, e.g. Coturnix coturnix and Perdix perdix. Therefore this community should deserve a high conservation priority for biodiversity. The factors that offer the greatest threats to the continuing existence of this community type are intensive agricultural management and the increasing spread of Ambrosia artemisiifolia.

Can management intensity be more important than environmental factors? A case study along an extreme elevation gradient from central Italian cereal fields

This paper aims to assess the importance of environmental and management factors determining the weed species composition along a strong elevation gradient. A total of 76 cereal fields (39 low input and 37 intensively managed) were sampled along an elevation gradient in central Italy. Explanatory variables were recorded for each field to elucidate the role of large-scale spatial trends, of site-specific abiotic environmental conditions and of field management characters. Redundancy analysis was used to assess the relative importance of each environmental variable in explaining the variation in species composition. Our results indicate that variation in weed species composition is strongly determined by altitude, mean annual precipitation, mean annual temperature and also by soil characteristics. However, the level of intensification proved to be the most influential variable. There was a significant difference in species richness and composition between low-input and intensively managed fields. Intensification leads to considerable species loss at both lower and higher elevations. Low-input fields had 296 species in total, while intensively managed fields had only 196.

Current distribution and spreading of Euphorbia davidii (E. dentata agg.) in Europe

Of the Euphorbia dentata aggregate, both E. dentata and E. davidii have been reported from Europe. A revision of herbarium and published materials, however, indicates that only E. davidii can be confirmed in Europe. Although known in Europe since 1968, most records are from the 1990s. There are 46 known populations scattered throughout the continent, in Russia, Ukraine, Moldova, Romania, Bulgaria, Hungary, Italy, Belgium, Switzerland and France. Most populations are small and restricted to railway areas; however, the species can also invade agricultural fields. Due to its wide distribution, the growing number of recently established populations, and its invasive ability, special attention should be paid to changes in distribution and population sizes in Europe.

Use of mesotrione and tembotrione herbicides for post-emergence weed control in alkaloid poppy (Papaver somniferum)

Two field experiments were carried out a year apart on the alkaloid poppy (Papaver somniferum) in 2012 and 2013 in north-west Hungary, to assess the efficacy of mesotrione and tembotrione herbicides for post-emergence weed control. Our experiments tested (1) a single application of mesotrione at 144 g active ingredient (a.i.) ha–1, (2) two separate applications of mesotrione at 144 g a.i. ha–1, (3) a single application of tembotrione at 88 g a.i. ha–1, (4) two separate applications of mesotrione at 88 g a.i. ha–1, and (5) the combination of a single application of mesotrione at 144 g a.i. ha–1 followed by a single application of tembotrione at 88 g a.i. ha–1. Both non-treated and hand-weeded plots were used as controls. Among the most important weeds, Chenopodium album was most successfully controlled in the majority of the test treatments, but Fallopia convolvulus and Polygonum aviculare tolerated each herbicide application in 2012, likely due to the dry weather conditions. Because of the botanical similarity to the crop, none of the treatments proved to be significant against Papaver rhoeas. One dosage of tembotrione alone never reduced the dry weights of the target weed species or weed numbers significantly. The cuticular wax layer of the opium poppy can provide a natural defence against these herbicides, but some temporary phytotoxic yellowish discoloration occurred after tembotrione treatments. Our results show that mesotrione in combination with tembotrione is the most effective treatment and should be employed in poppy cultivation.

Diterpene Constituents of Euphorbia exigua L. and Multidrug Resistance Reversing Activity of the Isolated Diterpenes.

Phytochemical investigation of the MeOH extract obtained from the aerial parts of the annual weed Euphorbia exigua L. resulted in the isolation of two novel (1, 2) and one known (3) jatrophane diterpenes. Their structures were established by extensive 1D‐ and 2D‐NMR spectroscopy and HR‐ESI‐MS. The isolated compounds were evaluated for multidrug resistance (MDR) reversing activity on human MDR gene‐transfected L5178 mouse lymphoma cells; and all three compounds were found to modulate the intracellular drug accumulation.

Abietane diterpenoids from Sideritis montana L. and their antiproliferative activity

The present study aimed at the phytochemical and pharmacological investigation of Sideritis montana L. (Lamiaceae). Two new abietane diterpenes [sideritins A (1) and B (2)] were isolated from the methanol extract of the plant. Six known compounds [pomiferin E (3), 9α,13α-epi-dioxyabiet-8(14)-en-18-ol (4), paulownin (5), 6-methoxysakuranetin (6), 3-oxo-α-ionol (7) and 4-allyl-2,6-dimethoxyphenol glucoside (8)] were also obtained from the plant. The structures were determined by means of HREIMS and NMR experiments. The antiproliferative effect of the isolated compounds was investigated on human cancer cell lines (HeLa, SiHa and C33A) at 10 and 30μM concentrations, using the MTT assay. The results demonstrated that pomiferin E (3) and 6-methoxysakuranetin (6) displayed considerable activity [inhibition (%)±SEM: 46.93±2.35 on HeLa (pomiferin E), and 51.52±2.45 on C33A (6-methoxysakuranetin)] at 30μM concentration.