Hajime Yasui

Micronucleus test with ethyl methanesulfonate administered by intraperitoneal injection and oral gavage

The effect of route of administration on the outcome of the micronucleus test was studied by administering ethyl methanesulfonate (EMS) by oral gavage (p.o.) and intraperitoneal injection (i.p.) to males of 2 mouse strains, MS/Ae and CD-1. Based on preliminary studies, consisting of a small-scale acute toxicity test and a pilot experiment to determine the optimal sampling time and the appropriate dosages, a micronucleus test was conducted with a 24-h sampling time and doses of 50-400 mg/kg i.p. and p.o. EMS significantly induced micronucleated polychromatic erythrocytes (MNPCEs) with a clear positive dose response by both routes in both strains. Moreover, both routes showed almost the same induction rate of MNPCEs at each dose level tested in both strains.

Studies on the Amylases of Paecilomyces subglobosus :Part I. Mycological Properties of Paecilomyces subglobosus sp. nov. TPR-3810 and -3811

Two strains of molds, which had a very close similarity with each other, and the acid-stable α-amylase activity of which was very high, were isolated from the air. Studies on the morphology of these strains revealed that, although they were fairly close to Paecilomyces varioti, they had some peculiar characteristics, and that their existence had not been reported hitherto. Therefore the authors concluded that the strains must belong to a new species in the genus Paecilomyces, and named them Paecilomyces subglobosus sp. nov. TPR-3810 and -3811, respectively, after their characteristic shape of the conidiospores.