Tetsuo Misaki

Simulatneous determination of baicalein, wogonin, oroxylin-A and their glucuronides in scutellariae radix by ion-pair high-performance liquid chromatography

Abstract A new, simple and precise analytical method using ion-pair high-performance liquid chromatography was developed for simultaneous of six flavonoids, namely baicalin, baicalein, wogonin, wogonin-glucuronide, oroxylin-A and oroxylin-A-glucuronide, in Scutellariae Radix. A reversed-phase chromatographic system consisting of a chemically bonded ODS silica gel column and water—acetonitrile (68:32), containing 5mM tetra-n-amylammonium bromide and adjusted to pH 4.0 by phosphoric acid, as mobile phase was used. The six flavonoids in this crude drug were completely separated within 25 min. Oroxylin-A and its glucuronide were separated for the first time. The analytical results for various samples are described.

Studies on the Protease Constitution of Aspergillus oryzae: Part I. Systematic Separation and Purification of Proteases

In order to elucidate the protease constitution of Aspergillus oryzae, systematic separation of proteases was elaborated by sequential chromatography on Amberlite CG–50, DEAE-Sephadex A–50 and CM-cellulose. As the results, three kinds of proteases, that is, acid-, neutral- and alkaline proteases were isolated and purified in crystalline form except neutral one. Purified neutral protease could not be crystallized, but was confirmed to be homogeneous by ultracentrifugal analysis. Besides these proteases, a new protease which was unknown up to the present in the constitution of Asp. oryzae proteases, was first isolated and designated as “semi-alkaline protease” according to its optimal pH.

Studies on the Amylases of Paecilomyces subglobosus :Part I. Mycological Properties of Paecilomyces subglobosus sp. nov. TPR-3810 and -3811

Two strains of molds, which had a very close similarity with each other, and the acid-stable α-amylase activity of which was very high, were isolated from the air. Studies on the morphology of these strains revealed that, although they were fairly close to Paecilomyces varioti, they had some peculiar characteristics, and that their existence had not been reported hitherto. Therefore the authors concluded that the strains must belong to a new species in the genus Paecilomyces, and named them Paecilomyces subglobosus sp. nov. TPR-3810 and -3811, respectively, after their characteristic shape of the conidiospores.