Hera Maheshwari

Haematological study of fruit bat, Cynopterus Tithaecheilus

Haematological profile is important to determine the physiological condition of an individual, and morphology of red blood cells can also be helpful in making a diagnosis of a disease. The size of red blood cells affects the ability of red blood cells to carry oxygen. In some clinical events shape and size of red blood cells will be changed. Bats are the only mammals that have the ability to fly. The ability of bats to fly would require bats to be able to bind more oxygen, therefor bat should have enough of energy while flying. The present study was conducted on 12 Cynopterus tithaecheilus (7 males and 5 females) to study about haematological profile. Blood samples were taken using tuberculin needle and analyzed by hematology analyzer for hematological value. Size of red blood cells was taken using bloods smear and analyzed by imageJ software. Cynopterus tithaecheilus show the higher number of red blood cells, but the size were smaller compared to the other mammals which has same body size. Mean while the concentration of hemoglobin and hematocrit were higher. The present result suggested that these haematological values might provide a greater transport oxygen capacity in the Cynopterus tithaecheilus.

Kesetaraan Uji Mastitis IPB-1 dengan Metode Breed untuk Mendiagnosis Mastitis Subklinis pada Susu Kerbau Murrah dan Kambing (THE EQUALITY OF IPB-1 MASTITIS TEST WITH BREED METHOD FOR SUB-CLINICAL MASTITIS DETECTION ON MURRAH BUFFALO’S MILK AND GOAT’S MILK

Sub-clinical mastitis causes decrease in milk production and milk quality. It is not only happen tomilking dairies, but also happens to dairy buffalos and goats. The objective of this study is to know thedifferences between IPB-1 mastitis test (IMT) and Breed method to diagnose sub-clinical mastitis ondairy buffaloes and goats. Fourty two samples of buffalo’s milk and 20 samples of goat’s milk were used tosomatic cell count (SCC) with direct and indirect method. Direct method was performed by counting themilk’s SCC with Breed method, and indirect method was performed by observing the reaction betweenIMT reagent and milk. The results showed that 28 from 42 samples (66.67%) of buffalo’s milk and 13 from20 samples (65%) of goat’s milk tested with Breed method came from the herds which suffered from subclinicalmastitis and 27 from 42 samples (64.28%) and 10 from 20 samples (50%) of goat’s milk testedwith IMT showed positive reaction. This research also showed that IMT has sensitivity of 96% and specivicityof 100% for buffalo’s milk and sensitivity of 71% and specivicity of 100% for goat’s milk. IMT can be usedto obtain fast result for sub-clinical mastitis diagnosis and it is faster and easier for buffalo’s and goat’smilk.

Diurnal Patterns of Testosterone and Cortisol Metabolites in Fecal of Javan Gibbons (Hylobates moloch) in Captivity

The term of allelopathy refers to chemical interactions (inhibitory or stimulatory) between plants, between plants and microorganisms, and between microorganisms. The wealth of information on the processes, procedures, and practices of allelopathy has contributed to understanding this field of science. Recently, researches of allelopathy have been conducted in laboratory, greenhouse, and field with multifaceted standpoint in some concerning area: (i) allelochemicals identifications and screening test; (ii) ecological and physiological aspects of allelopathy; (iii) genetic studies and the possibilities of using plant breeding or genetic manipulation to enhance allelopathic varieties; (iv) the use of allelopathic potential in the biological control, including as natural pesticide, of weeds and plant diseases as eco-friendly approach for sustainable agriculture scheme. Key words: Allelochemicals, biological control, sustainable agriculture

Pola Diurnal Metabolit Testosteron dan Kortisol di dalam Feses Owa Jawa (Hylobates moloch) di Penangkaran

The aims of this research were to determine diurnal patterns of testosterone and cortisol metabolites to predict the testis functional status. In this study, fecal testosterone and cortisol were quantified in 77 samples from three male Hylobates moloch during a course of three months period. These data showed that the highest concentration of fecal testosterone occured at 18.00-06.00 (23.61 ng/g dried feces), then declined gradually. The lowest concentration was in the evening (5.54 ng/g dried feces). Our tests showed that there was a decrease in the mean testosterone concentration from 06.00-10.00 to 10.00-14.00 to 14.00-18.00. For cortisol, the highest concentration occured at 06.00-10.00 (597.84 ng/g dried feces), then decline gradually in the evening (225.73 ng/g dried feces).