Hideyo Nagaoka

Pharmacokinetics of High Dose Methotrexate : 1 : In Acute Renal Failure

Acute renal failure developed in a 5 year-old girl during the fifth course of high dose methotrexate (MTX) treatment for osteosarcoma. She showed oliguria, and the serum MTX concentration, BUN and Scr were 461 μmoles/1, 50 mg/dl and 2.6 mg/dl, respectively, at 27 hr after infusion.Massive leucovorin calcium rescure, direct hemoperfusion, hemodialysis and plasma exchange therapies were successfully treated and she recovered gradually.Neither toxic bone marrow suppression nor side effect of massive leucovorin calcium appeared.The pharmacokinetic analysis of MTX during the renal failure and the dialysis treatment was investigated.

Dissolution rates and blood concentration of griseofulvin in rabbits.

Weight variation test, disintegration test, and dissolution test were made as quality tests for 6 different preparations of griseofulvin. In the dissolution test, water/ethanol (1: 2, v/v) mixture and double-layer solution of water/n-octanol (1:1), water/benzene (1: 1) or water/ethyl acetate (1: 1) were used as dissolution mediums. The absorption of griseofulvin was studied by measuring the blood concentration in rabbits.The dissolution rates and the parameters of the blood levels were different among the preparations. However, the correlation was observed between the. results of the dissolution test using the water/n-octanol solution as dissolution medium and the AUC-time curve calculated from the blood concentration in rabbits.