Hilal Irmak Sapmaz

Beneficial effects of montelukast against methotrexate-induced liver toxicity: a biochemical and histological study.

The effects of montelukast against methotrexate-induced liver damage were investigated. 35 Wistar albino female rats were divided into 5 groups as follows: group I: control; group II: montelukast (ML); group III: methotrexate (Mtx); group IV: montelukast treatment after methotrexate application (Mtx + ML); group V: montelukast treatment before methotrexate application (ML + Mtx). At the end of the experiment, the liver tissues of rats were removed. Malondialdehyde (MDA), myeloperoxidase (MPO), and reduced glutathione levels were determined from liver tissues. In addition, the liver tissues were examined histologically. MDA and MPO levels of Mtx group were significantly increased when compared to control group. In Mtx + ML group, these parameters were decreased as compared to Mtx group. Mtx injection exhibited major histological alterations such as eosinophilic staining and swelling of hepatocytes. The glycogen storage in hepatocytes was observed as decreased by periodic acid schiff staining in Mtx group as compared to controls. ML treatment did not completely ameliorate the lesions and milder degenerative alterations as loss of the glycogen content was still present. It was showed that montelukast treatment after methotrexate application could reduce methotrexate-induced experimental liver damage.

Beneficial Effects of Montelukast against Cisplatin-Induced Acute Renal Damage in Rats

Objective: In this study, the therapeutic and protective effects of montelukast against cisplatin (CP)-induced acute renal damage were investigated. Materials and Methods: Thirty-five female rats were divided into five groups as follows: (1) control, (2) montelukast (10 mg/kg daily for 10 days per-oral (p.o.), (3) CP (single dose 7 mg/kg intraperitoneally (i.p.)), (4) CP + montelukast (10 mg/kg daily for 10 days p.o., after 3 days of the injection of CP), (5) montelukast (10 mg/kg daily for 10 days p.o.) + CP (single dose 7 mg/kg i.p., after the last dose of montelukast). At the end of the experiment, malondialdehyde (MDA), a lipid peroxidation product, myeloperoxidase (MPO), and reduced glutathione (GSH) levels were determined in the renal tissue. Also, blood urea nitrogen (BUN) and creatinine (Cr) levels were assayed from the trunk blood samples. Results: CP treatment caused a significant elevation of MDA, MPO, BUN, and Cr levels when compared with the control group. Also, GSH levels were found to be reduced due to the CP treatment. Montelukast administration after CP injection ameliorated all of these parameters. Our histopathological findings (marked swelling of epithelial cells, tubular dilatation, tubular desquamation, and loss of brush border in the kidney) were consistent with the biochemical results. Conclusion: Montelukast treatment after CP injection exerted therapeutic effects against CP-induced acute kidney damage.

Effects of formaldehyde inhalation on humoral immunity and protective effect of Nigella sativa oil An experimental study

Aim: This study was carried out to determine the effects of formaldehyde (FA) inhalation on the humoral immunity of rats and the protective effect of Nigella sativa (NS) oil. Materials and Methods: The rats (n = 33) were divided into five groups, with five animals in the control group (FA-free air) and seven in the other four groups. Group FA1 was exposed to FA (5 ppm), group FA + NS1 was treated with NS and exposed to FA (5 ppm), group FA2 was exposed to FA (10 ppm), and group FA + NS2 was treated with NS and exposed to FA (10 ppm). At the end of a 4-week study period, blood samples were collected. Enzyme-linked immunosorbent assay was used to determine the levels of serum total immunoglobulin A (IgA), total immunoglobulin M (IgM), total immunoglobulin G (IgG), and complement 3 (C3). Results: FA inhalation significantly increased serum IgA, IgM, and C3 levels and decreased serum IgG levels compared with the control group. NS administration decreased serum IgA, IgM, and C3 levels, which were induced by FA inhalation. Conclusion: FA inhalation significantly increased acute antibody responses and C3 levels in a dose-dependent manner compared with the control group. FA inhalation decreased the secondary immune response compared with the control group. Levels of acute antibody responses and complement following exposure to FA inhalation returned to normal following treatment with NS (immunoregulatory effect). However, NS did not affect the secondary immune response.

HSP70 immune reactivity and TUNEL positivity in the liver of toluene-inhaled and melatonin-treated rats.

Toluene is a clear, colorless and volatile hydrocarbon that is metabolized in liver, produced free oxygen radicals and can mediate cellular damage. Melatonin which is a pineal gland hormone is a very potent antioxidant. It can make the cellular membrane more durable against oxidative attacks and protect nuclear DNA from oxidative damage. This study aimed to investigate heat shock protein (HSP)70 immune reactivity and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) positivity (apoptotic activity) in the liver of toluene-inhaled and melatonin-treated rats. A total of 21 adult male Wistar albino rats were divided at random into 3 equal groups. Animals in group I were designated as control. The rats in group II were exposed to toluene (3000 ppm/1 h/day) for 30 days, while the rats in group III were treated with melatonin (10 mg/kg/day, intraperitoneally) plus toluene inhalation. At the end of the 30-day experimental period, all rats were killed by decapitation. Then the liver tissues of rats were removed and tissue specimens were embedded in paraffin blocks. The specimens were stained with periodic acid-schiff (PAS) following routine histological procedures. Sections obtained from paraffin blocks were used for immune detection of TUNEL and HSP70. In light microscopic observations of tissues from toluene-inhaled rats, massive hepatocyte degeneration, ballooning degeneration and decreased PAS positivity were observed. Increased TUNEL positivity and HSP70 immune reactivity were determined in toluene-inhaled group and melatonin treatment decreased all these adverse effects.

Impact of Hard Palate Angulation Caused by Septal Deviation on Maxillary Sinus Volume

Objective To investigate the effect of hard palate angulation caused by septal deviation on the volume of the maxillary sinus. Methods Coronal computed tomographic (CT) scans of 1568 patients aged from 18 to 60 were examined. CT scans of 402 patients were included in the study. On these scans, the maxillary sinus volume, the angle of the nasal septal deviation, and the angulation of the hard palate were calculated using the ImageJ software. Each maxillary sinus volume was statistically compared with each other and with those in the control group. Correlations between palatal angulation and septal deviation were determined. Results Deviated nasal septum whether with or without deflection of the hard palate was noted to have caused changes in the volume of the maxillary sinus in both female and male patients. The volume of the maxillary sinus on the deviated side was less than that of the opposite side, and the differences between the volumes of both sinuses were statistically significant (p<0.05). No significant differences were noted when compared with the control group. A positive correlation was observed between the nasal septal deviation angle and the angulation of the hard palate. Conclusion Regardless of whether or not it affects the hard palate, nasal septal deviation reduces the volume of the maxillary sinus on the deviated side but does not affect the total volume of the maxillary sinuses. Significant differences between the volumes on the two sides can lead to facial asymmetry.

Epidemiological investigation of traumatic upper extremity fractures in children who applied to emergency department

A B S T R A C T Introduction: Serious and potentially fatal multi-trauma in childhood can rarely be seen. However, each one of 5 children is exposed to minor trauma . Our aim is to reveal upper extremity traumas, trauma mechanisms and seasonal relationship in children under 18 years of age. Patients and methods: In this retrospective study, the files and graphs of 500 patients under 18 years old who applied to Tokat Gaziosmanpasa University Medical Faculty Emergency Service after trauma were examined through Enlil and PACS system. 328 patients with upper extremity pathology were included in the study. These patients were analyzed according to different age groups (0-4,5-8,9-12,13-17). Results: 500 patients were screened and 328 patients (65.6%) with upper extremity trauma were included. 109 (33.23%) patients were female and 209 (66.77%) patients were male. The most frequent 0-4 year upper extremity traumas (n = 39) in girls were found to decrease with age. In boys, it was found that the upper extremity traumas increased with age, the most common age was 13-17 (n = 79). The distal radius fracture (n = 64) and distal humerus fracture (n = 61) were the most common in upper extremity traumas. The highest falling down in the etiology of 328 patients with upper extremity pathology (n = 149, 45.43%) was detected. It was determined that traumatic upper extremity pathologies were followed most in summer (36.58%) followed by autumn, spring and winter respectively. Conclusion: Two - thirds of children under 18 years old who applied for trauma were admitted due to upper extremity trauma. It was found that fracture of the distal radius (n = 87) was the most (33.85%) when the isolated radius fractures, bilateral forearm fractures, and upper extremity multiple fractures were evaluated together in children.

The role of chrysin against harmful effects of formaldehyde exposure on the morphology of rat fetus liver and kidney development

This study was aimed to investigate possible harmful effects of formaldehyde (FA) exposure on the morphology of fetus liver and kidney development during pregnancy and also to determinate possible protective role of chrysin (CH) against these harmful effects. For this aim, after pregnancy was induced, 58 female rats were divided into 6 groups. Serum physiologic (SF) was injected to the Group I rats intraperitoneally (i.p.). 20 mg/kg CH was given to the Group II via gavage. 0.1 mg/kg FA was applied to the Group III (i.p.), 1 mg/kg FA was injected to Group IV (i.p.) 0.1 mg/kg FA was given to Group V i.p., and 20 mg/kg CH was given to the same group via gavage. 1 mg/kg FA was applied to Group VI i.p., and 20 mg/kg CH was given to the same group via gavage. Fetuses were taken from each pregnant rat with cesarean section on the 20th day of the pregnancy. The morphological analyses of the fetuses, liver and kidney; biochemical and histological analyses of the liver and kidney were performed. The fetal body, liver and kidney weight of the FA groups demonstrated a statistically significant decrease the compared to control group. Also the FA-1 group were observed histopathological changes on the fetus liver and kidneys. FA exposure causes harmful effects on fetus the liver and kidneys. CH reduces the negative effect on morphological variables statistically. Although CH is insufficient to fix the histopathological changes that occur in the liver, damaging effects that occur in the kidney decreased statistically.

Spontan Subdural Hematomu Olan Genç Hasta, Olgu Sunumu

Acil servise norolojik sikayetlerle basvurunun oldukca sik sebeplerinden biri bas agrisidir. Subdural hematom genellikle yaslilarda gorulur. Genclerde subdural hematom nadirdir ve genellikle travma sebebiyle olusur. Genclerde spontan subdural hematoma ise cok nadiren rastlanir. Bu olguda acil servise bas agrisi sikayeti ile basvuran 35 yasinda erkek hastayi sunduk. Hastanin travma oykusu yoktu. Cerrahi sonrasi hasta tam duzelme ile taburcu edildi.