Hongwu Zhang

Trichodermone, a spiro-cytochalasan with a tetracyclic nucleus (7/5/6/5) skeleton from the plant endophytic fungus Trichoderma gamsii.

Trichodermone (1), the first spiro-cytochalasan with an unprecedented tetracyclic nucleus (7/5/6/5), together with its possible biosynthetic precursor aspochalasin D (2), was isolated from the endophytic fungus Trichoderma gamsii. Compound 2 displayed moderate inhibitory activity against HeLa cells with an IC50 value of 5.72 μM.

Trichalasins C and D from the plant endophytic fungus Trichoderma gamsii

Two new cytochalasans, trichalasins C (1) and D (2) together with known cytochalasans aspochalasins D (3), M (4) and P (5) were isolated from one endophytic fungus Trichoderma gamsii inhabiting in traditional medicinal plant Panax notoginseng (BurK.) F.H.Chen. The structures for the new compounds 1 and 2 were determined by NMR and HRESIMS, and their relative configurations were established by analysis of coupling constants and NOESY correlations. Compound 3 displaying inhibitory activity with EC₅₀ value 5.72 μM, whereas the EC₅₀ values for compounds 1, 2 and 4, 5 are more than 40 μM.

Inositol angelates from the whole herb of Inula cappa

A new inositol derivative, cis-1,2,3,5-trans-4,6-inositol-2,3,6-triangelate(1) along with the known l-inositol-1,2,3,5-tetra angelate(2), was isolated from the ethanolic extract of the whole plant Inula cappa and identified on the spectroscopic analysis.

Cytochalasans with different amino-acid origin from the plant endophytic fungus Trichoderma gamsii

Cytochalasans with different amino-acid origin from the plant endophytic fungus Trichoderma gamsii

Sesquiterpenes and Cyclopeptides from the Endophytic Fungus Trichoderma asperellumSamuels, Lieckf. & Nirenberg

Trichodones A–C (1–3, resp.), three new sesquiterpenes, together with the two known cyclopeptides PF1022F and halobacillin (4 and 5, resp.), have been isolated from the crude extract of the endophytic fungus Trichoderma asperellum Samuels, Lieckf. & Nirenberg residing in the traditional Chinese medicinal plant Panax notoginseng (Burkill) F.H.Chen. The structures and configurations of compounds 1–3 were determined by NMR spectroscopy and mass spectrometry. The relative configuration for 1 was determined by analysis of its pertinent coupling constants and NOESY correlations, whereas the absolute configuration of 1 was established by CD spectroscopy. Compounds 4 and 5 displayed antibacterial activities against Enterococcus faecium (CGMCC 1.2025) with IC50 values of 7.30 and 5.24u2005μm, and against Staphylococcus aureus COL (CGMCC 1.2465) with IC50 values of 19.02 and 14.00u2005μM, respectively.

Genome-wide analysis of the expression profile of Saccharomyces cerevisiae in response to treatment with the plant isoflavone, wighteone, as a potential antifungal agent

The limitations of currently available antifungal agents and the rapid emergence of drug-resistant strains necessitate more efficient approaches to screening and developing novel antifungal drugs. The antifungal activity of the natural products of a series of plants was evaluated and wighteone, 5, 7, 4′–trihydroxy–6–(γ,γ-dimethylallyl)isoflavone showed excellent anti-yeast activity (MIC against Saccharomyces cerevisiae was 4xa0μg/ml). Transcriptome profiling of wighteone-treated S. cerevisiae indicated that wighteone is different from commonly used antifungal compounds in its mode of action.

Two new triterpene saponins from Lysimachia davurica.

Two new saponins, named davuricoside L (1) and davuricoside O (2), have been isolated from the whole plants of Lysimachia davurica. Their structures were determined by 1D and 2D NMR, FAB-MS techniques, and chemical methods.

Trichoderpyrone, a Unique Polyketide Hybrid with a Cyclopentenone–Pyrone Skeleton from the Plant Endophytic Fungus Trichoderma gamsii

Trichoderpyrone (1), a unique polyketide with a cyclopentenone-pyrone hybrid skeleton, was isolated from the plant endophytic fungus Trichoderma gamsii. The structure of 1 was determined by detailed analysis of NMR data together with comparison of chemical shift values of similar fragments. The relative and absolute configurations were established by NOESY correlations and CD experiment. Trichoderpyrone (1) displayed weak cytotoxic activities against A549, HepG2, and HeLa cancer cell lines. 1 might originate from a hybrid biosynthetic pathway through two nonreduced (NR) polyketide megasynthetases.

Polyketides with different post-modifications from desert endophytic fungus Paraphoma sp.

Abstract Three new polyketides 4,6,8-trihydroxy-5-methyl-3,4-dihydronaphthalen-1(2H)-one (1), 5,7-dihydroxy-3-(1-hydroxyethyl)-3,4-dimethylisobenzofuran-1(3H)-one (2) and 1-(4-hydroxy-6-methoxy-1,7-dimethyl-3-oxo-1,3-dihydroisobenzofuran-1-yl) ethyl acetate (3) together with seven known analogues (4–10) were isolated from desert endophytic fungus Paraphoma sp. The structures of these compounds were elucidated by analysis of NMR data. The absolute configuration of (1–3) was established on the basis of CD experiments. The possible biosynthetic pathway of compounds (1–10) was suggested, which implied that these secondary metabolites might be originated from polyketide biosynthesis with different post-modification reactions. Compounds 2, and 5–8 were evaluated for bioactivities against plant pathogen A. solani, whereas none of them displayed any biological effects. In addition, compounds 1, 2 and 5–10 were also tested for cytotoxic activities against three human cancer cell lines (HepG2 cells, MCF-7 cells and Hela cells) without biological effects.