Iacir Francisco dos Santos

Caracterização das lesões por Cysticercus bovis, na inspeção post mortem de bovinos, pelos exames macroscópico, histopatológico e pela reação em cadeia da polimerase (PCR)

Considering the importance of improving methods for diagnosis of bovine Cysticercosis, this study aimed to verify Cysticercus bovis occurrence in different anatomical sites, as head, heart, esophagus, diaphragm, tongue, liver and carcass, examined by federal inspection service. Diagnosis was performed by gross examination, histopatholgy and PCR with boiling DNA extraction for metacestode identification. Of 22043 slaughtered cattle, 713 (3.23%) were infected. The heart was mostly affected with 1.90% (420/22043), followed by head, 1.11% (245/22043), esophagus, 0.08% (18/22043), carcass, 0.07% (15/22043), diaphragm, 0.03% (7/22043), liver, 0.02% (5/22043) and tongue, 0.01% (3/22043). Of the cysts obtained, 58.35% (416/713) were dead and 41.65% (297/713) were alive. The differences among anatomical sites and cysts status were significant (p<0.05). Of the 416 dead cysts 253, characterized by nodular firm whitish lesions, containing yellowish material, some times in calcareous aspect were examined for histopathology. The histological exams of these cysts yielded granulomatous lesions, whose centers were characterized by caseous and/or calcareous material, multinucleate giant cells, histiocytes in palisade and infiltrate composed predominantly by lymphoid cells, wrapped up by fibrosis. Some times the lesions peripheries had granulation tissue and mineralized areas, like linear blade. The parasite debris were like a hyaline, non cellular material with spherical and ovoid, basophilic, eosinophilic and colorless corpuscles. These corpuscles were seen rarely, some times, among inflammatory reaction. Fibrous nodules, rich in lymphoid or mixed infiltrates, were frequently seen. Of the live cysts subjected to PCR with boiling DNA extraction, 65% (13/20) were positive for C. bovis, confirming the ambulatory diagnosis and the efficacy of the PCR procedure used. Due to microscopic and PCR diagnostic exams of C. bovis, mainly in the liver and esophagus, it is suggested changes in the 176 article of the regulatory inspection, by including these sites in the bovine routine inspection at the slaughterhouses.

Bacterial microbiota present in the gallbladder of cattle and antimicrobial resistance of Staphylococcus isolates

Microrganismos patogenicos podem residir temporariamente ou permanentemente na vesicula biliar de bovinos. Assim, durante o abate, maior atencao deve ser dada ao trato gastrointestinal, especialmente para o orgao acessorio, a vesicula biliar. O principal objetivo deste estudo foi caracterizar a microbiota bacteriana presente na bile e no epitelio de vesiculas biliares de bovinos abatidos em matadouro frigorifico sob inspecao sanitaria e avaliar a resistencia antimicrobiana de estirpes do genero Staphyloccocus. Foram coletadas 30 vesiculas biliares integras e foi pesquisada na bile e no epitelio do orgao a presenca de bacterias heterotroficas aerobias mesofilas (BHAM), Staphylococcus spp. e Enterobacteriaceae totais, Escherichia coli, Enterococcus spp. e Salmonella spp. A frequencia de isolamento dos microrganismos citados acima foi, respectivamente: 23,02%, 14,39%, 13,67%, 24,46%, 0% e 24,46%. Em relacao aos dois ambientes da vesicula, a frequencia de isolamento dos microrganismos no epitelio foi de 64,03%, e na bile 35,97%, nao sendo diferente estatisticamente, mas com diferenca significativa entre as medias populacionais. No teste de susceptibilidade aos antimicrobianos, as estirpes de Staphylococcus isoladas a partir da bile e do epitelio da vesicula biliar apresentaram sensibilidade a: penicilina G, ceftriaxona, cloranfenicol e gentamicina. A observacao de que a vesicula biliar comporta microrganismos em elevadas frequencias atenta para o fato de que o bovino possa ser um portador persistente de patogenos de grande importância em saude publica.