Krishna N. Reddy

Factors affecting Campsis radicans seed germination and seedling emergence

Abstract The effects of environmental factors on germination and emergence of Campsis radicans seeds were examined in laboratory and greenhouse experiments. Campsis radicans pods produced numerous, papery, and small seeds (696 seeds/pod; 4 mg/seed). Seeds exhibited dormancy that was relieved (74% germination) after 2 wk of prechilling. Fluctuating temperatures and a 12-h photoperiod were required for maximum germination. Optimum conditions for C. radicans seed germination (74%) were 35/25 C (day/night, 12/12 h) with a 12-h photoperiod. Temperatures below 25/15 C or above 40/30 C were unfavorable for germination. Germination in constant temperatures or in continuous darkness was less than 15%. More than 59% of C. radicans seeds germinated at pH 5 to 9, but at pH 4 or 10 seed germination was totally inhibited. Germination was totally inhibited at osmotic stress higher than −0.2 MPa. Germination was 60% at 40 mM NaCl and 20% at 160 mM NaCl. Emergence was maximum (68%) for seeds that were placed on the soil surface, but no seedlings emerged from a soil depth at 4 cm. About 10% of seeds were still viable even after 20 wk of prechilling. Each pod contained about 700 seeds and each plant produced 20 to 40 pods. These results suggest that the spread potential of C. radicans by seeds would be at least 1,400 to 2,800 seeds plant−1. However, only seeds near the soil surface would be able to germinate. Nomenclature: Trumpetcreeper; Campsis radicans (L.) Seem. ex Bureau CMIRA.

Role of absorption and translocation in the mechanism of glyphosate resistance in horseweed (Conyza canadensis)

Abstract Greenhouse and laboratory experiments were conducted to investigate mechanisms of glyphosate resistance in horseweed populations from Mississippi, Arkansas, Delaware, and Tennessee. A nondestructive leaf-dip bioassay was developed to confirm resistance and susceptibility in individual test plants. A single leaf was excised from each plant, and the petiole and bottom one-fourth of leaf was dipped in a 600 mg ae L−1 glyphosate solution for 2 d followed by visually estimating the injury on a scale of 0 to 10. Plants were classified as resistant (R) if the score was 2 to 3 and susceptible (S) if the score was 5 to 6. 14C-glyphosate solution was applied on the adaxial surface of a fully expanded leaf of the second whorl of four-whorl rosette plants. Plants were harvested 48 h after treatment and radioactivity was determined in treated leaf, other leaves, crown, and roots. Absorption of 14C-glyphosate was similar (47 to 54%) between R and S plants from within and among the four states, suggesting absorption is not involved in glyphosate resistance. The amount of radioactivity translocated from the treated leaf was reduced in R plants compared with S plants. The reduction in translocation of 14C-glyphosate ranged from 28% in Mississippi-R biotype to 48% in Delaware-R biotype compared with their respective S biotypes. Epicuticular wax mass ranged from 6 to 80 μg cm−2 among horseweed biotypes, with no differences between R and S biotypes within each state. Treating two leaves with glyphosate solution at the field use rate (0.84 kg ae ha−1) killed S plants but not R plants (38 to 58% control) regardless of state origin. These results suggest that a simple bioassay can be used to screen biotypes for suspected resistance and that reduced translocation of glyphosate plays a major role in glyphosate resistance in R biotypes of horseweed. Nomenclature: 14C-glyphosate; glyphosate; horseweed, Conyza canadensis (L.) Cronq.

Factors affecting germination of horseweed (Conyza canadensis)

Abstract The influence of environmental factors on germination and emergence of horseweed was examined in growth chamber experiments. Germination was highest (61%) under 24/20 C day/night temperature under light. Horseweed seed germination was observed under both light (13 h photoperiod) and complete darkness (24 h), but germination under continuous darkness was only 0 to 15% compared with 0 to 61% under light. All other experiments were conducted under 24/20 C and 13-h light conditions. Germination was 19 to 36% over a pH range from 4 to 10, with a trend toward higher germination under neutral-to-alkaline conditions. Horseweed germination was > 20% at < 40 mM NaCl concentration and lowest (4%) at 160 mM NaCl. These data suggest that even at high soil salinity conditions, horseweed can germinate. Germination of horseweed decreased from 25% to 2% as osmotic potential increased from 0 (distilled water) to −0.8 MPa, indicating that germination can still occur under moderate water stress conditions. Horseweed seedling emergence was at its maximum on the soil surface, and no seedlings emerged from seeds placed at a depth of 0.5 cm or higher. Nomenclature: Horseweed, Conyza canadensis (L.) Cronq. ERICA.

Factors affecting seed germination, seedling emergence, and survival of texasweed (Caperonia palustris)

Abstract Field, laboratory, and greenhouse experiments were conducted to determine the seed production potential and effect of environmental factors on germination, emergence, and survival of texasweed. Texasweed produced an average of 893 seed per plant, and 90% were viable. Seed exhibited dormancy, and prechilling did not release dormancy. Percent germination ranged from 56% for seed subjected to no prechilling to 1% for seed prechilled at 5 C for 140 d. Seed remained viable during extended prechilling conditions, with 80% of seed viable after 140 d of prechilling. Texasweed seed germinated over a range of 20 to 40 C, with optimum germination (54%) occurring with a fluctuating 40/30 C temperature regime. Seed germinated with fluctuating 12-h light/dark and constant dark conditions. Texasweed seed germinated over a broad range of pH, osmotic potential, and salt concentrations. Seed germination was 31 to 62% over a pH range from 4 to 10. Germination of texasweed ranged from 9 to 56% as osmotic potential decreased from − 0.8 MPa to 0 (distilled water). Germination was greater than 52% at less than 40 mM NaCl concentrations and lowest (27%) at 160 mM NaCl. Texasweed seedlings emerged from soil depths as deep as 7.5 cm (7% emergence), but emergence was > 67% for seed placed on the soil surface or at a 1-cm depth. Texasweed seed did not germinate under saturated or flooded conditions, but seed survived flooding and germinated (23 to 25%) after flood removal. Texasweed seedlings 2.5 to 15 cm tall were not affected by emersion in 10-cm-deep flood for up to 14 d. These results suggest that texasweed seed is capable of germinating and surviving in a variety of climatic and edaphic conditions, and that flooding is not a viable management option for emerged plants of texasweed. Nomenclature: Texasweed, Caperonia palustris (L.) St. Hil. CNPPA.

Effects of Cereal and Legume Cover Crop Residues on Weeds, Yield, and Net Return in Soybean (Glycine max)1

A 2-yr field study was conducted during 1998 and 1999 at Stoneville, MS, on a Dundee silt loam to determine weed control, yield, and net return associated with winter cover crops in soybean. Cover crop systems included Italian ryegrass, oat, rye, wheat, hairy vetch, crimson clover, subterranean clover, no-cover crop conventional tillage (CT), and no-cover crop no-tillage (NT), all with standard preemergence (PRE), postemergence (POST), PRE + POST, and no-herbicide weed management. Oat (11.1 Mg/ha) had highest dry biomass compared to all other cover crops (6.0 to 7.6 Mg/ha) at soybean planting. Biomass decreased 9 wk after planting (WAP) compared to the respective biomass at soybean planting in all cover crops. Italian ryegrass and rye biomass decay was slow and about two-thirds of plant residue persisted at 9 WAP. Cover crops had no effect on densities of barnyardgrass, prickly sida, and yellow nutsedge, but altered the density of browntop millet. Total weed biomass was higher in rye, wheat, and subterranean clover than in Italian ryegrass cover crop systems, and higher with the PRE-only vs. POST-only or PRE + POST programs at 10 WAP soybean. Soybean yield decreased in the order of no-cover crop NT ≥ no-cover crop CT ≥ hairy vetch ≥ crimson clover ≥ rye ≥ oat ≥ wheat ≥ subterranean clover > Italian ryegrass. None of the cover crop systems gave soybean yield higher than the no-cover crop CT system in the absence of herbicides. Under a PRE-only program, all cover crop systems had lower yield compared to the no-cover crop CT system. When late-emerged weeds were controlled with POST applications (POST-only or PRE + POST programs), all cover crops, except Italian ryegrass, had no detrimental effect on soybean yields, which were not different from no-cover crop CT plots. In cover crops, input costs were high due to additional cost of seeds, planting, and desiccation. Net return was highest in no-cover crop NT (

Weed control and economic comparisons of glyphosate-resistant, sulfonylurea-tolerant, and conventional soybean (Glycine max) systems.

105/ha) followed by no-cover crop CT (

Germination and emergence of hairy beggarticks (Bidens pilosa).

76/ha) system. Net returns were negative for all cover crops and losses were highest in crimson clover (−

Aminomethylphosphonic acid accumulation in plant species treated with glyphosate.

62/ha) and subterranean clover (−

Glyphosate Tolerance Mechanism in Italian Ryegrass (Lolium multiflorum) from Mississippi

161/ha). Nomenclature: Barnyardgrass, Echinochloa crus-galli (L.) Beauv. #3 ECHCG; browntop millet, Brachiaria ramosa (L.) Stapf # PANRA; prickly sida, Sida spinosa L. # SIDSP; yellow nutsedge, Cyperus esculentus L. # CYPES; crimson clover, Trifolium incarnatum L. ‘Dixie’; hairy vetch, Vicia villosa Roth; Italian ryegrass, Lolium multiflorum Lam. ‘Gulf’; oat, Avena sativa L. ‘Bob’; rye, Secale cereale L. ‘Elbon’; soybean, Glycine max (L.) Merr. ‘DP 3588’; subterranean clover, Trifolium subterraneum L. ‘Mount Barker’; wheat, Triticum aestivum L. ‘Cocker 9803’. Additional index words: Allelopathy, conventional tillage, herbicide, integrated weed management, mulch, net return, no-tillage, weed emergence, weed biomass. Abbreviations: ANOVA, analysis of variance; CT, conventional tillage; NT, no-tillage; POST, postemergence; PRE, preemergence; WAP, weeks after planting soybean.

Herbicide efficacy, leaf structure, and spray droplet contact angle among Ipomoea species and smallflower morningglory

Abstract: A field study was conducted over 2 yr to compare efficacy and economics of glyphosate-resistant, sulfonylurea-tolerant, and conventional soybean (Glycine max) weed control programs. Herbicide programs in the three soybean systems provided at least 90% control of browntop millet (Brachiaria ramosa), prickly sida (Sida spinosa), yellow nutsedge (Cyperus esculentus), pitted morningglory (Ipomoea lacunosa), and hemp sesbania (Sesbania exaltata) in most cases and postemergence (POST)-only programs were as effective as preemergence (PRE) followed by POST programs. Control of hyssop spurge (Euphorbia hyssopifolia) ranged from 93 to 100% in glyphosate-resistant soybean and from 88 to 100% in conventional soybean, but control was 60 to 100% in sulfonylurea-tolerant soybean. Sicklepod (Senna obtusifolia) control was at least 91% in glyphosate-resistant and sulfonylurea-tolerant soybean but was 81% for the standard SAN 582 plus imazaquin PRE and acifluorfen plus bentazon early POST treatment in conventional soybean. In glyphosate-resistant soybean, glyphosate applied sequentially resulted in an average yield of 3,020 kg/ha with a net return of