Ida Fitriana

The structural and functional recovery of pancreatic β-cells in type 1 diabetes mellitus induced mesenchymal stem cell-conditioned medium

Aim: Various studies have shown that secreted factors alone in culture medium without stem cell are capable of repairing tissues by itself in various conditions involving damaged tissue/organ. Therefore, this study was aimed to investigate the role of human umbilical cord mesenchymal stem cell-derived conditioned medium (CM) on the recovery of pancreatic β-cells in Wistar rats (Rattus norvegicus) with type 1 diabetes mellitus. Materials and Methods: The 0.05 ml CM induction was applied to the diabetic group of rats in weeks 1, 2, 3, and 4. 1 week after each CM induction, insulin concentration was analyzed using ELISA. The pancreas was divided into 3 regions, processed by paraffin method, stained with hematoxylin-eosin, and immunohistochemical method for insulin. Results: This study indicated the decrease in the total number of islets and insulin concentration after the injection of single dose of alloxan. The exocrine acini were also damaged. Microscopic observation detected the presence of small islets in the diabetic group 1 week after the first 0.05 ml CM induction. The number and size of the islets increased in line with the CM doses and time of inductions. Immunohistochemically, the presence of low intensity of insulin-positive cells could be recognized at the splenic and duodenal regions of the pancreas, but not gastric region, 1 week after the first and second 0.05 ml CM induction. The intensity of staining and the number of insulin-positive cells increased dramatically in 1 week after the third and fourth 0.05 ml of CM induction in all regions of the pancreas. The data of insulin blood concentration showed clear differences between the second and the fourth induction of 0.05 ml CM induction. Conclusions: This study showed very strong evidence on the role of human umbilical cord mesenchymal stem cell-derived CM in recovering the pancreatic β-cells damage in Wistar rats (R. norvegicus) with type 1 diabetes mellitus, structurally and functionally.

ARIMA implementation to predict the amount of antiseptic medicine usage in veterinary hospital

The medicine is a tool for patients either human or animal to treat or prevent disease. One of the medicines which often used to help prevent infections is an antiseptic medicine. Povidone-iodine is an antiseptic and disinfectant medicine that is extensively used by hospital and other health care. A health care institution such as pharmacies or hospitals must ensure the availability of medicines for patients. These things make the pharmaceutical dealing with the uncertainty of the medicine needed. Veterinary hospital as a health service provider has several challenges. One of the challenges is it must ensure the availability of the medicine needed at all time. The predictive capability of the amount of medicine usage can assist for managing the availability of medicines in veterinary hospitals. This study analyses and presents a forecasting model using ARIMA method to predict an antiseptic povidone-iodine usage at Prof. Soeparwi Veterinary Hospital. ARIMA method was selected because the method can be used in fitting forecasting models when there is a non-stationary time series and the method has capability to correct for local trend in data. Results of this study is ARIMA (1,0,1) was selected as a suitable model to represent historical dataset.

Management information systems development for veterinary hospital patient registration using first in first out algorithm

Registration system is one of the most important elements in an organization or institution that involves the presence of customers and one by one services. Nowadays, with the development of technology, services process in an institution become more effective and efficient. One of the institutions that require the development of this technology, in the form of hospital information management system, is the veterinary hospital. The conventional system has many shortcomings include allowing an error in writing the patient data also the patient registration recaps is less effective and more time consuming. Therefore, a prototype of RSH management information system was made as an ilustration in the design of hospital information management system. The focus of this study is the patient registration system which use First In First Out (FIFO) algorithms where the patient who came first to the hospital is the one who enrolled first.