İnan Kaya

Protective effect of omega-3 fatty acid against mercury chloride intoxication in mice.

The aim of this study was to investigate the protective effect of omega-3 fatty acid in HgCI2 toxicity in mice. Levels of malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO) and total sialic acid (TSA), and histopathological changes in selected organs were evaluated. Twenty-eight mice were equally divided into 4 groups, namely Groups I-IV. Group I animals received intraperitoneal (ip) injection of physiological saline solution; Group II animals received ip injection of 0.4mg/kg/day HgCI2; Group III animals received ip injection of 0.4mg/kg/day HgCI2 in addition to subcutaneous (sc) injection of 0.5g/kg/day omega-3 fatty acid; and Group IV animals received sc injection of 0.5g/kg/day omega-3 fatty acid. All treatments lasted 7 days. The levels of MDA, NO and TSA were significantly higher in Group II and lower in Groups III and IV as compared to the Group I. GSH level was the highest in Group IV. In histopathology, severe degeneration in liver and kidney was observed in Group II animals. These degrading changes were seen to be reduced greatly in Group III animals. The results suggested that omega-3 fatty acid might attenuate HgCI2-induced toxicity by improving antioxidant status and acute phase response in mice.

Investigation of Serum Oxidative Stress Index and Paraoxonase Activity Levels in Colostrum Period of Dairy Cows

In this study, the investigation of serum oxidative stress index (OSI), paraoxonase 1 (PON1) activity, total antioxidant (TAS) and oxidant status (TOS) levels in colostrum period of dairy cows was aimed. It was used sixteen Simmental cows for blood samples as follows: within 0, 1, 2, 3 and 4 days postpartum (group I, II, III, IV and V, respectively). Serum TAS, TOS and PON1 activity levels were determined by spectrophotometric methods. The results showed that oxidative stress in group IV was higher than group I and II. PON1 activity in group V was significantly higher than other groups. It was concluded that serum oxidative stress and PON1 activity levels in colostric period of dairy cows changed daily.