Irum Sabir Ali
Lady Reading Hospital
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Publication
Featured researches published by Irum Sabir Ali.
Journal of Breast Cancer | 2016
Arshad Ali; Farman Ullah; Irum Sabir Ali; Ahmad Faraz; Mumtaz Khan; Syed Tahir Ali Shah; Nawab Ali; Muhammad Saeed
Purpose The promoter methylation status of cell cycle regulatory genes plays a crucial role in the regulation of the eukaryotic cell cycle. CpG cytosines are actively subjected to methylation during tumorigenesis, resulting in gain/loss of function. E2F5 gene has growth repressive activities; various studies suggest its involvement in tumorigenesis. This study aims to investigate the epigenetic regulation of E2F5 in breast cancer to better understand tumor biology. Methods The promoter methylation status of 50 breast tumor tissues and adjacent normal control tissues was analyzed. mRNA expression was determined using SYBR® green quantitative polymerase chain reaction (PCR), and methylation-specific PCR was performed for bisulfite-modified genomic DNA using E2F5-specific primers to assess promoter methylation. Data was statistically analyzed. Results Significant (p<0.001) upregulation was observed in E2F5 expression among tumor tissues, relative to the control group. These samples were hypo-methylated at the E2F5 promoter region in the tumor tissues, compared to the control. Change in the methylation status (Δmeth) was significantly lower (p=0.022) in the tumor samples, indicating possible involvement in tumorigenesis. Patients at the postmenopausal stage showed higher methylation (75%) than those at the premenopausal stage (23.1%). Interestingly, methylation levels gradually increased from the early to the advanced stages of the disease (p<0.001), which suggests a putative role of E2F5 methylation in disease progression that can significantly modulate tumor biology at more advanced stage and at postmenopausal age (Pearsons r=0.99 and 0.86, respectively). Among tissues with different histological status, methylation frequency was higher in invasive lobular carcinoma (80.0%), followed by invasive ductal carcinoma (46.7%) and ductal carcinoma in situ (20.0%). Conclusion Methylation is an important epigenetic factor that might be involved in the upregulation of E2F5 gene in tumor tissues, which can be used as a prognostic marker for breast cancer.
Archive | 2014
Z. Anwar; M. Azhar Khan; Irum Sabir Ali; M. Asghar; Muhammad Sher; Imran Shakir; Muhammad Sarfraz; M. Farooq Warsi
Journal of Ayub Medical College Abbottabad | 2013
Irum Sabir Ali; Mumtaz Khan; Muhammad Atif Khan
Jcpsp-journal of The College of Physicians and Surgeons Pakistan | 2012
Irum Sabir Ali; Mumtaz Khan; Atifullah Khan; Muhammad Fahd Shah; Gohar Ali
Journal of Postgraduate Medical Institute | 2011
Waqar Alam Jan; Irum Sabir Ali; Nadeem Ali Shah; Azra Ghani; Mumtaz Khan; Abdus Samad Khan
Pesquisa Agropecuaria Brasileira | 2017
Farman Ullah; Nawab Ali; Irum Sabir Ali; Shamim Saleha; Syed Tahir Ali Shah; Hazir Rahman; Muhammad S. Ahmad; Muhammad Saeed
Journal of Postgraduate Medical Institute | 2016
Ahmad Faraz; Irum Sabir Ali; Ihtisham Ul Haq; Mumtaz Khan
Archive | 2015
Ahmad Faraz; Fazal Bari; Irum Sabir Ali; Hamzullah Khan; Amjad Naeem; Mumtaz Khan; Abid Hussain; Khyber Girls
Archive | 2014
Ar Hakeem; Ashbala Shakoor; Irum Sabir Ali; M. Azhar Khan; B. M. Naeem Ashiq; Mohammad Ishaq; Amer Aziz
Journal of Postgraduate Medical Institute | 2011
Zafar Iqbal; Irum Sabir Ali; Mumtaz Khan